Helicobacter pylori is gram-negative bacteria may transmit through human food chain causing serious health problems in humans worldwide upon misusing antibiotic therapy for animals. Aim of the study represent the incidence of variants biotypes H. pylori susceptible to antibiotic in apparently healthy or clinical illness felines and sheep through amplification of 16srRNA. Four stomachs of 3 apparently healthy and diarrheal feline and two stool of constipated and pan-leukopenia felines, in addition to five gastric sheep from 2 normal and 3 congested plus one milk, selected from 52 felines and 83 sheep, respectively based on traditional cultivation and biochemical differentiation in total twelve H. pylori isolates that confirmed by amplification of 16srRNA, not being recognized by v3-v4 primer as nitrate gram negative bacteria. H. pylori isolates were grouped upon urease and nitrate reduction reaction in total percent 50% for each of weak and strong urease biotypes, including 33.3% & 66.6% for (+ve) or (-ve) nitrate reductive biotypes, respectively. Sensitivity of these biotypes was determined against fourteen antibiotic discs by antimicrobial susceptibility test to find highest sensitivity non-producing nitrate biotypes from felines is (87.5%), lesser than sheep (100%) but almost positive nitrate reductive isolates is less susceptible in percent 25%. Moderate sensitivity of weak urease biotypes represents 50% against amikacin, opposite to strong urease isolates (33.3%). Highest sensitivity strong urease biotypes show 83.3% against clarithromycin and levofloxacin, compared to weak urease biotypes 50 & 66.6%, respectively. Bio-typing H. pylori is preferable for programming eradication in molecular surveying normal or clinical illness animals.
| Published in | Animal and Veterinary Sciences (Volume 12, Issue 1) |
| DOI | 10.11648/avs.20241201.15 |
| Page(s) | 37-47 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Antimicrobial Susceptibility Test (AST), H. pylori, Nitrate, Urease, 16srRNA, Sheep and Felines
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APA Style
Enany, M. E. S., Fadel, H. M., Abdelsadek, U. H. A., Ahmed, M. M. M., Kholief, M. E. A. G. (2024). Insight of Molecular Prevalence on Antibiotic Sensitive H. pylori Biotypes from Apparently Healthy and Clinical Illness Felines and Sheep. Animal and Veterinary Sciences, 12(1), 37-47. https://doi.org/10.11648/avs.20241201.15
ACS Style
Enany, M. E. S.; Fadel, H. M.; Abdelsadek, U. H. A.; Ahmed, M. M. M.; Kholief, M. E. A. G. Insight of Molecular Prevalence on Antibiotic Sensitive H. pylori Biotypes from Apparently Healthy and Clinical Illness Felines and Sheep. Anim. Vet. Sci. 2024, 12(1), 37-47. doi: 10.11648/avs.20241201.15
AMA Style
Enany MES, Fadel HM, Abdelsadek UHA, Ahmed MMM, Kholief MEAG. Insight of Molecular Prevalence on Antibiotic Sensitive H. pylori Biotypes from Apparently Healthy and Clinical Illness Felines and Sheep. Anim Vet Sci. 2024;12(1):37-47. doi: 10.11648/avs.20241201.15
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Download@article{10.11648/avs.20241201.15,
author = {Mohamed El Sayed Enany and Hanaa Mohamed Fadel and Usama Hassan Abo-Shama Abdelsadek and Mona Muhammad Mahmoud Ahmed and Mohamed Ezat Abdel Gaied Kholief},
title = {Insight of Molecular Prevalence on Antibiotic Sensitive H. pylori Biotypes from Apparently Healthy and Clinical Illness Felines and Sheep},
journal = {Animal and Veterinary Sciences},
volume = {12},
number = {1},
pages = {37-47},
doi = {10.11648/avs.20241201.15},
url = {https://doi.org/10.11648/avs.20241201.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.avs.20241201.15},
abstract = {Helicobacter pylori is gram-negative bacteria may transmit through human food chain causing serious health problems in humans worldwide upon misusing antibiotic therapy for animals. Aim of the study represent the incidence of variants biotypes H. pylori susceptible to antibiotic in apparently healthy or clinical illness felines and sheep through amplification of 16srRNA. Four stomachs of 3 apparently healthy and diarrheal feline and two stool of constipated and pan-leukopenia felines, in addition to five gastric sheep from 2 normal and 3 congested plus one milk, selected from 52 felines and 83 sheep, respectively based on traditional cultivation and biochemical differentiation in total twelve H. pylori isolates that confirmed by amplification of 16srRNA, not being recognized by v3-v4 primer as nitrate gram negative bacteria. H. pylori isolates were grouped upon urease and nitrate reduction reaction in total percent 50% for each of weak and strong urease biotypes, including 33.3% & 66.6% for (+ve) or (-ve) nitrate reductive biotypes, respectively. Sensitivity of these biotypes was determined against fourteen antibiotic discs by antimicrobial susceptibility test to find highest sensitivity non-producing nitrate biotypes from felines is (87.5%), lesser than sheep (100%) but almost positive nitrate reductive isolates is less susceptible in percent 25%. Moderate sensitivity of weak urease biotypes represents 50% against amikacin, opposite to strong urease isolates (33.3%). Highest sensitivity strong urease biotypes show 83.3% against clarithromycin and levofloxacin, compared to weak urease biotypes 50 & 66.6%, respectively. Bio-typing H. pylori is preferable for programming eradication in molecular surveying normal or clinical illness animals.
},
year = {2024}
}
TY - JOUR T1 - Insight of Molecular Prevalence on Antibiotic Sensitive H. pylori Biotypes from Apparently Healthy and Clinical Illness Felines and Sheep AU - Mohamed El Sayed Enany AU - Hanaa Mohamed Fadel AU - Usama Hassan Abo-Shama Abdelsadek AU - Mona Muhammad Mahmoud Ahmed AU - Mohamed Ezat Abdel Gaied Kholief Y1 - 2024/02/05 PY - 2024 N1 - https://doi.org/10.11648/avs.20241201.15 DO - 10.11648/avs.20241201.15 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 37 EP - 47 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/avs.20241201.15 AB - Helicobacter pylori is gram-negative bacteria may transmit through human food chain causing serious health problems in humans worldwide upon misusing antibiotic therapy for animals. Aim of the study represent the incidence of variants biotypes H. pylori susceptible to antibiotic in apparently healthy or clinical illness felines and sheep through amplification of 16srRNA. Four stomachs of 3 apparently healthy and diarrheal feline and two stool of constipated and pan-leukopenia felines, in addition to five gastric sheep from 2 normal and 3 congested plus one milk, selected from 52 felines and 83 sheep, respectively based on traditional cultivation and biochemical differentiation in total twelve H. pylori isolates that confirmed by amplification of 16srRNA, not being recognized by v3-v4 primer as nitrate gram negative bacteria. H. pylori isolates were grouped upon urease and nitrate reduction reaction in total percent 50% for each of weak and strong urease biotypes, including 33.3% & 66.6% for (+ve) or (-ve) nitrate reductive biotypes, respectively. Sensitivity of these biotypes was determined against fourteen antibiotic discs by antimicrobial susceptibility test to find highest sensitivity non-producing nitrate biotypes from felines is (87.5%), lesser than sheep (100%) but almost positive nitrate reductive isolates is less susceptible in percent 25%. Moderate sensitivity of weak urease biotypes represents 50% against amikacin, opposite to strong urease isolates (33.3%). Highest sensitivity strong urease biotypes show 83.3% against clarithromycin and levofloxacin, compared to weak urease biotypes 50 & 66.6%, respectively. Bio-typing H. pylori is preferable for programming eradication in molecular surveying normal or clinical illness animals. VL - 12 IS - 1 ER -
Microbiology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
