Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production

Nguyen Thi Hoa; Tang Thi Chinh; Dang Thi Mai Anh; Ngo Dinh Binh; Le Thi Minh Thanh

doi:doi:10.11648/j.ajaf.20140205.12

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Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production

Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh

Published in American Journal of Agriculture and Forestry (Volume 2, Issue 5)

Received: 11 August 2014 Accepted: 28 August 2014 Published: 10 September 2014

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Abstract

Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.

Published in	American Journal of Agriculture and Forestry (Volume 2, Issue 5)
DOI	10.11648/j.ajaf.20140205.12
Page(s)	219-225
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Bacillus thuringiensis, Delta endotoxin, Dewatered Wastewater Sludge, Bio-Pesticide

References

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Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. (2014). Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. American Journal of Agriculture and Forestry, 2(5), 219-225. https://doi.org/10.11648/j.ajaf.20140205.12

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ACS Style

Nguyen Thi Hoa; Tang Thi Chinh; Dang Thi Mai Anh; Ngo Dinh Binh; Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am. J. Agric. For. 2014, 2(5), 219-225. doi: 10.11648/j.ajaf.20140205.12

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AMA Style

Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am J Agric For. 2014;2(5):219-225. doi: 10.11648/j.ajaf.20140205.12

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@article{10.11648/j.ajaf.20140205.12,
  author = {Nguyen Thi Hoa and Tang Thi Chinh and Dang Thi Mai Anh and Ngo Dinh Binh and Le Thi Minh Thanh},
  title = {Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production},
  journal = {American Journal of Agriculture and Forestry},
  volume = {2},
  number = {5},
  pages = {219-225},
  doi = {10.11648/j.ajaf.20140205.12},
  url = {https://doi.org/10.11648/j.ajaf.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140205.12},
  abstract = {Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.},
 year = {2014}
}

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TY  - JOUR
T1  - Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production
AU  - Nguyen Thi Hoa
AU  - Tang Thi Chinh
AU  - Dang Thi Mai Anh
AU  - Ngo Dinh Binh
AU  - Le Thi Minh Thanh
Y1  - 2014/09/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajaf.20140205.12
DO  - 10.11648/j.ajaf.20140205.12
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 219
EP  - 225
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20140205.12
AB  - Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.
VL  - 2
IS  - 5
ER  -

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Author Information

Nguyen Thi Hoa

Dept of Environmental Microbiology, Institute of Environmental Technology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
Tang Thi Chinh

Dept of Environmental Microbiology, Institute of Environmental Technology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
Dang Thi Mai Anh

Dept of Environmental Microbiology, Institute of Environmental Technology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam
Ngo Dinh Binh

Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
Le Thi Minh Thanh

Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

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Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. (2014). Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. American Journal of Agriculture and Forestry, 2(5), 219-225. https://doi.org/10.11648/j.ajaf.20140205.12

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Nguyen Thi Hoa; Tang Thi Chinh; Dang Thi Mai Anh; Ngo Dinh Binh; Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am. J. Agric. For. 2014, 2(5), 219-225. doi: 10.11648/j.ajaf.20140205.12

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AMA Style

Nguyen Thi Hoa, Tang Thi Chinh, Dang Thi Mai Anh, Ngo Dinh Binh, Le Thi Minh Thanh. Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production. Am J Agric For. 2014;2(5):219-225. doi: 10.11648/j.ajaf.20140205.12

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@article{10.11648/j.ajaf.20140205.12,
  author = {Nguyen Thi Hoa and Tang Thi Chinh and Dang Thi Mai Anh and Ngo Dinh Binh and Le Thi Minh Thanh},
  title = {Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production},
  journal = {American Journal of Agriculture and Forestry},
  volume = {2},
  number = {5},
  pages = {219-225},
  doi = {10.11648/j.ajaf.20140205.12},
  url = {https://doi.org/10.11648/j.ajaf.20140205.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140205.12},
  abstract = {Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.},
 year = {2014}
}

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TY  - JOUR
T1  - Optimization of Fermentation Medium Compositions from Dewatered Wastewater Sludge of Beer Manufactory for Bacilus Thuringiensis Delta Endotoxin Production
AU  - Nguyen Thi Hoa
AU  - Tang Thi Chinh
AU  - Dang Thi Mai Anh
AU  - Ngo Dinh Binh
AU  - Le Thi Minh Thanh
Y1  - 2014/09/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ajaf.20140205.12
DO  - 10.11648/j.ajaf.20140205.12
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 219
EP  - 225
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20140205.12
AB  - Optimization of medium compositions from dewatered wastewater sludge of beer manufactory for delta endotoxin production by Bacillus thuringiensis was investigated in flask fermentation. Its composition consisted of wastewater sludge hydrolysis broth with several agricultural byproducts (rice bran, soybean meal, corn flour) and mineral salts (MgSO4.7H2O; K2HPO4; KH2PO4; MnSO4; CaCl2; NaCl). Rice bran and MgSO4.7H2O, CaCl2 were all found to have a significant influence on delta endotoxin production. The optimal concentration of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for delta endotoxin production were determined as follows: WWS (20 g/l, dry weight), CaCl2 (0.3 g/l), MgSO4.7H2O (0.32 g/l), and rice bran (5.8 g/l). Using this optimized fermentation media, the yield of delta endotoxin was increased by 34% to 565 mg/l compared with unoptimal medium. Viable cell and spore counts obtained in optimum fermented broth were 1.25x109 CFU/ml, 1.07x109 CFU/ml, respectively. The LC50 value of Bacillus thuringiensis serovar isralensis against Culex quinquefasciatus was 0.056 mg/l and there was no significant difference between LC50 value of bacterium grown in the medium.
VL  - 2
IS  - 5
ER  -

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