Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics

Kishor Chand Kumhar; Azariah Babu; Mitali Bordoloi; Ashif Ali

doi:doi:10.11648/j.ajaf.20140206.24

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Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics

Kishor Chand Kumhar, Azariah Babu, Mitali Bordoloi, Ashif Ali

Published in American Journal of Agriculture and Forestry (Volume 2, Issue 6)

Received: 27 November 2014 Accepted: 22 December 2014 Published: 5 February 2015

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Abstract

The Genus Trichoderma is of immense importance in agricultural crop protection because of their bio-control potential role against an array of phytopathogens through several modes of action. It is well established with fairly good acceptability, worldwide. The establishment and utilization on a commercial level of any promising isolate may not be successful, unless the cost effective mass production is evident. Present study is aimed at the evaluation of the laboratory media as well as locally available food grains for cost effective mass production of local strain KBN-24 (Trichoderma viride) for large scale adoption. Among different lab media, potato dextrose agar (solid medium) and potato dextrose broth (liquid medium) yielded comparatively more biomass of tested strain of Trichoderma viride. However, among the different grains rice ranked the first which produced the maximum biomass (148.04 gram) followed by wheat (126.87 gram) where as maize produced the least biomass. Similar trends were recorded on the conidial production and colony forming units (CFUs) in case of potato dextrose agar, potato dextrose broth and rice whole grain. Results indicated that the locally available food grains like rice and wheat were comparatively cheaper and serve as convenient substrates for the mass multiplication of Trichoderma viride and their cost economics were also discussed.

Published in	American Journal of Agriculture and Forestry (Volume 2, Issue 6)
DOI	10.11648/j.ajaf.20140206.24
Page(s)	317-320
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Trichoderma Viride, Mass Multiplication, Culture Media, Production Economics

References

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[2]	F.C. Dominguesa, J.A. Queiroza, J. M. S. Cabralb and L. P. Fonsecab. (2000) The influence of culture conditions on mycelial structure and cellulose production by Trichoderma reesei rut C-30. Enz. Microbial Technol. 26: 394-401.
[3]	B. K. Borthakur and P. Dutta. (2011) Disease management in tea, In Tea field management, Tea Research Association, Tocklai Experimental Station, Jorhat -785 008, Assam, India, pp. 182-188.
[4]	E. Esposito and M. da Silva. (1998) Systematics and environmental application of the genus Trichoderma. Crit. Rev. Microbiol. 24:89-98.
[5]	G.S. Kocher, K.L. Kalra, G. and Banta. (2008) Optimization of cellulase production by submerged fermentation of rice straw by Trichoderma harzianum Rut-C 8230. Int. J. Microbiol. 5(2): 8230.
[6]	N. Subash, J. Viji, C. Sasikumar, and M. Meenakshisundaram. (2013) Isolation, media optimization and formulation of Trichoderma harzianum in agricultural soil. Journal of Microbiology and Biotechnology Research.3 (1): 61-64.
[7]	Mridula Khandelwal, Sakshi Datta, Jitendra Mehta, Ritu Naruka, Komal Makhijani, Gajendra Sharma, Rajesh Kumar and Subhas Chandra. (2012) Isolation, characterization & biomass production of Trichoderma viride using various agro products- A biocontrol agent. Advances in Applied Science Research, 2012, 3 (6):3950-3955.
[8]	D. Ajay, J. S. Bisen, M. Singh, R. Saha and B. Bera. (2013) Evaluation of various plant residues in tea plantations of Darjeeling for on farm production of Trichoderma harzianum. Indian Journal of research and practices. 1: 1-4.
[9]	Prashant Pingolia, Rohini Maheshwari, Narendra Vaishnav, Gajendra P. Sharma and Jitendra Mehta. (2013) Mycelium growth of Trichoderma viride (Biocontrol agent) on different agar medium. International Journal of Recent Biotechnology. 1 (1): 43-47.
[10]	G. C. Papavizas, M. T. Dunn, J. A. Lewis and J. Beagle-Ristaino. (1984) Liquid fermentation technology for experimental production of biocontrol fungi. Phytopathology. 74:1171-1175.
[11]	K. Nagur Babu, and P.N. Pallavi. (2013) Isolation, identification and mass multiplication of Trichoderma an important bio-control agent. International Journal of pharmacy and life sciences. 4(1): 2320-2323.
[12]	P. J. Chaudhari, Prashant Shrivastava and A. C. Khadse. (2011) Substrate evaluation for mass cultivation of Trichoderma viride. Asiatic Journal of Biotechnology Resources. 2(04): 441-446.

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APA Style

Kishor Chand Kumhar, Azariah Babu, Mitali Bordoloi, Ashif Ali. (2015). Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics. American Journal of Agriculture and Forestry, 2(6), 317-320. https://doi.org/10.11648/j.ajaf.20140206.24

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ACS Style

Kishor Chand Kumhar; Azariah Babu; Mitali Bordoloi; Ashif Ali. Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics. Am. J. Agric. For. 2015, 2(6), 317-320. doi: 10.11648/j.ajaf.20140206.24

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AMA Style

Kishor Chand Kumhar, Azariah Babu, Mitali Bordoloi, Ashif Ali. Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics. Am J Agric For. 2015;2(6):317-320. doi: 10.11648/j.ajaf.20140206.24

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@article{10.11648/j.ajaf.20140206.24,
  author = {Kishor Chand Kumhar and Azariah Babu and Mitali Bordoloi and Ashif Ali},
  title = {Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics},
  journal = {American Journal of Agriculture and Forestry},
  volume = {2},
  number = {6},
  pages = {317-320},
  doi = {10.11648/j.ajaf.20140206.24},
  url = {https://doi.org/10.11648/j.ajaf.20140206.24},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140206.24},
  abstract = {The Genus Trichoderma is of immense importance in agricultural crop protection because of their bio-control potential role against an array of phytopathogens through several modes of action. It is well established with fairly good acceptability, worldwide. The establishment and utilization on a commercial level of any promising isolate may not be successful, unless the cost effective mass production is evident. Present study is aimed at the evaluation of the laboratory media as well as locally available food grains for cost effective mass production of local strain KBN-24 (Trichoderma viride) for large scale adoption. Among different lab media, potato dextrose agar (solid medium) and potato dextrose broth (liquid medium) yielded comparatively more biomass of tested strain of Trichoderma viride. However, among the different grains rice ranked the first which produced the maximum biomass (148.04 gram) followed by wheat (126.87 gram) where as maize produced the least biomass. Similar trends were recorded on the conidial production and colony forming units (CFUs) in case of potato dextrose agar, potato dextrose broth and rice whole grain. Results indicated that the locally available food grains like rice and wheat were comparatively cheaper and serve as convenient substrates for the mass multiplication of Trichoderma viride and their cost economics were also discussed.},
 year = {2015}
}

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TY  - JOUR
T1  - Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics
AU  - Kishor Chand Kumhar
AU  - Azariah Babu
AU  - Mitali Bordoloi
AU  - Ashif Ali
Y1  - 2015/02/05
PY  - 2015
N1  - https://doi.org/10.11648/j.ajaf.20140206.24
DO  - 10.11648/j.ajaf.20140206.24
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 317
EP  - 320
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20140206.24
AB  - The Genus Trichoderma is of immense importance in agricultural crop protection because of their bio-control potential role against an array of phytopathogens through several modes of action. It is well established with fairly good acceptability, worldwide. The establishment and utilization on a commercial level of any promising isolate may not be successful, unless the cost effective mass production is evident. Present study is aimed at the evaluation of the laboratory media as well as locally available food grains for cost effective mass production of local strain KBN-24 (Trichoderma viride) for large scale adoption. Among different lab media, potato dextrose agar (solid medium) and potato dextrose broth (liquid medium) yielded comparatively more biomass of tested strain of Trichoderma viride. However, among the different grains rice ranked the first which produced the maximum biomass (148.04 gram) followed by wheat (126.87 gram) where as maize produced the least biomass. Similar trends were recorded on the conidial production and colony forming units (CFUs) in case of potato dextrose agar, potato dextrose broth and rice whole grain. Results indicated that the locally available food grains like rice and wheat were comparatively cheaper and serve as convenient substrates for the mass multiplication of Trichoderma viride and their cost economics were also discussed.
VL  - 2
IS  - 6
ER  -

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Author Information

Kishor Chand Kumhar

Tea Research Association, North Bengal Regional Research & Development Centre, Nagrakata, District – Jalpaiguri, West Bengal – 735 225, India
Azariah Babu

Tea Research Association, North Bengal Regional Research & Development Centre, Nagrakata, District – Jalpaiguri, West Bengal – 735 225, India
Mitali Bordoloi

Tea Research Association, North Bengal Regional Research & Development Centre, Nagrakata, District – Jalpaiguri, West Bengal – 735 225, India
Ashif Ali

Tea Research Association, North Bengal Regional Research & Development Centre, Nagrakata, District – Jalpaiguri, West Bengal – 735 225, India

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APA Style

Kishor Chand Kumhar, Azariah Babu, Mitali Bordoloi, Ashif Ali. (2015). Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics. American Journal of Agriculture and Forestry, 2(6), 317-320. https://doi.org/10.11648/j.ajaf.20140206.24

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ACS Style

Kishor Chand Kumhar; Azariah Babu; Mitali Bordoloi; Ashif Ali. Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics. Am. J. Agric. For. 2015, 2(6), 317-320. doi: 10.11648/j.ajaf.20140206.24

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AMA Style

Kishor Chand Kumhar, Azariah Babu, Mitali Bordoloi, Ashif Ali. Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics. Am J Agric For. 2015;2(6):317-320. doi: 10.11648/j.ajaf.20140206.24

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@article{10.11648/j.ajaf.20140206.24,
  author = {Kishor Chand Kumhar and Azariah Babu and Mitali Bordoloi and Ashif Ali},
  title = {Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics},
  journal = {American Journal of Agriculture and Forestry},
  volume = {2},
  number = {6},
  pages = {317-320},
  doi = {10.11648/j.ajaf.20140206.24},
  url = {https://doi.org/10.11648/j.ajaf.20140206.24},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140206.24},
  abstract = {The Genus Trichoderma is of immense importance in agricultural crop protection because of their bio-control potential role against an array of phytopathogens through several modes of action. It is well established with fairly good acceptability, worldwide. The establishment and utilization on a commercial level of any promising isolate may not be successful, unless the cost effective mass production is evident. Present study is aimed at the evaluation of the laboratory media as well as locally available food grains for cost effective mass production of local strain KBN-24 (Trichoderma viride) for large scale adoption. Among different lab media, potato dextrose agar (solid medium) and potato dextrose broth (liquid medium) yielded comparatively more biomass of tested strain of Trichoderma viride. However, among the different grains rice ranked the first which produced the maximum biomass (148.04 gram) followed by wheat (126.87 gram) where as maize produced the least biomass. Similar trends were recorded on the conidial production and colony forming units (CFUs) in case of potato dextrose agar, potato dextrose broth and rice whole grain. Results indicated that the locally available food grains like rice and wheat were comparatively cheaper and serve as convenient substrates for the mass multiplication of Trichoderma viride and their cost economics were also discussed.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Evaluation of Culture Media for Biomass Production of Trichoderma viride (KBN 24) and their Production Economics
AU  - Kishor Chand Kumhar
AU  - Azariah Babu
AU  - Mitali Bordoloi
AU  - Ashif Ali
Y1  - 2015/02/05
PY  - 2015
N1  - https://doi.org/10.11648/j.ajaf.20140206.24
DO  - 10.11648/j.ajaf.20140206.24
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 317
EP  - 320
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20140206.24
AB  - The Genus Trichoderma is of immense importance in agricultural crop protection because of their bio-control potential role against an array of phytopathogens through several modes of action. It is well established with fairly good acceptability, worldwide. The establishment and utilization on a commercial level of any promising isolate may not be successful, unless the cost effective mass production is evident. Present study is aimed at the evaluation of the laboratory media as well as locally available food grains for cost effective mass production of local strain KBN-24 (Trichoderma viride) for large scale adoption. Among different lab media, potato dextrose agar (solid medium) and potato dextrose broth (liquid medium) yielded comparatively more biomass of tested strain of Trichoderma viride. However, among the different grains rice ranked the first which produced the maximum biomass (148.04 gram) followed by wheat (126.87 gram) where as maize produced the least biomass. Similar trends were recorded on the conidial production and colony forming units (CFUs) in case of potato dextrose agar, potato dextrose broth and rice whole grain. Results indicated that the locally available food grains like rice and wheat were comparatively cheaper and serve as convenient substrates for the mass multiplication of Trichoderma viride and their cost economics were also discussed.
VL  - 2
IS  - 6
ER  -

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