Douvir–N is a combination of lamivudine, zidovudine and nevirapine used for the treatment of patients with Human Immunodeficiency Virus. The objective of this study was to investigate the effect of combined administration of Douvir–N and folic acid on the histology and some Biochemical parameters in the liver of Wistar rats. Forty adult albino Wistar rats were randomly divided into four groups of ten animals each. Group A served as control and were administered with 1ml of distilled water, group B animals were administered with 9.29mg/kg body weight of Douvir–N, Group C animals were administered with a combination of 9.29mg/kg of Douvir–N and 0.07mg/kg of folic acid. Animals in group D were administered with 0.07mg/kg of folic acid. Animals were sacrificed after 30 days and dissected. The liver was removed and fixed in 10% buffered formaldehyde, processed and stained using Haematoxylin and Eosin staining method, carcino-embryonic antigen (CEA) and cytokeratin-7 (CK-7) immunochemistry methods. Stained slides were viewed using light microscope. Blood samples from each rat was collected using syringes and needles, The sera were extracted into fresh test tubes and stored in a refrigerator for analysis of aspartate aminotransaminase test (AST), alanine aminotransaminase test (ALT), alkaline phosphotase (ALP). The liver of Wistar rats administered with Douvir–N showed distortions in the liver with moderate dilatation of the sinusoidal spaces and nuclei pyknotic changes, with increased expression of CEA and CK7 in the groups treated with Douvir–N than the control groups. There was a significant increase in ALP in the Douvir–N groups. These changes were ameliorated when Douvir–N was combined with Folic acid. The findings suggest that Douvir–N can distort the cytoarchitecture and Biochemical parameters of the liver which could be ameliorated by co-administration with folic acid. Folic acid should be given as adjuvant drug to patients on Douvir–N therapy.
| Published in | American Journal of BioScience (Volume 3, Issue 6) |
| DOI | 10.11648/j.ajbio.20150306.11 |
| Page(s) | 197-202 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Liver, Douvir–N, Folic Acid, Human Immunodeficiency Virus (HIV), Biochemical Parameters
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APA Style
Aniekan Imo Peter, Gabriel Joseph Ekandem. (2015). Immunohistopathological Effects of Combined Administration of Douvir–N and Folc Acid on the Liver and Some Biochemical Parameters in Albino Wistar Rats. American Journal of BioScience, 3(6), 197-202. https://doi.org/10.11648/j.ajbio.20150306.11
ACS Style
Aniekan Imo Peter; Gabriel Joseph Ekandem. Immunohistopathological Effects of Combined Administration of Douvir–N and Folc Acid on the Liver and Some Biochemical Parameters in Albino Wistar Rats. Am. J. BioScience 2015, 3(6), 197-202. doi: 10.11648/j.ajbio.20150306.11
AMA Style
Aniekan Imo Peter, Gabriel Joseph Ekandem. Immunohistopathological Effects of Combined Administration of Douvir–N and Folc Acid on the Liver and Some Biochemical Parameters in Albino Wistar Rats. Am J BioScience. 2015;3(6):197-202. doi: 10.11648/j.ajbio.20150306.11
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Download@article{10.11648/j.ajbio.20150306.11,
author = {Aniekan Imo Peter and Gabriel Joseph Ekandem},
title = {Immunohistopathological Effects of Combined Administration of Douvir–N and Folc Acid on the Liver and Some Biochemical Parameters in Albino Wistar Rats},
journal = {American Journal of BioScience},
volume = {3},
number = {6},
pages = {197-202},
doi = {10.11648/j.ajbio.20150306.11},
url = {https://doi.org/10.11648/j.ajbio.20150306.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150306.11},
abstract = {Douvir–N is a combination of lamivudine, zidovudine and nevirapine used for the treatment of patients with Human Immunodeficiency Virus. The objective of this study was to investigate the effect of combined administration of Douvir–N and folic acid on the histology and some Biochemical parameters in the liver of Wistar rats. Forty adult albino Wistar rats were randomly divided into four groups of ten animals each. Group A served as control and were administered with 1ml of distilled water, group B animals were administered with 9.29mg/kg body weight of Douvir–N, Group C animals were administered with a combination of 9.29mg/kg of Douvir–N and 0.07mg/kg of folic acid. Animals in group D were administered with 0.07mg/kg of folic acid. Animals were sacrificed after 30 days and dissected. The liver was removed and fixed in 10% buffered formaldehyde, processed and stained using Haematoxylin and Eosin staining method, carcino-embryonic antigen (CEA) and cytokeratin-7 (CK-7) immunochemistry methods. Stained slides were viewed using light microscope. Blood samples from each rat was collected using syringes and needles, The sera were extracted into fresh test tubes and stored in a refrigerator for analysis of aspartate aminotransaminase test (AST), alanine aminotransaminase test (ALT), alkaline phosphotase (ALP). The liver of Wistar rats administered with Douvir–N showed distortions in the liver with moderate dilatation of the sinusoidal spaces and nuclei pyknotic changes, with increased expression of CEA and CK7 in the groups treated with Douvir–N than the control groups. There was a significant increase in ALP in the Douvir–N groups. These changes were ameliorated when Douvir–N was combined with Folic acid. The findings suggest that Douvir–N can distort the cytoarchitecture and Biochemical parameters of the liver which could be ameliorated by co-administration with folic acid. Folic acid should be given as adjuvant drug to patients on Douvir–N therapy.},
year = {2015}
}
TY - JOUR T1 - Immunohistopathological Effects of Combined Administration of Douvir–N and Folc Acid on the Liver and Some Biochemical Parameters in Albino Wistar Rats AU - Aniekan Imo Peter AU - Gabriel Joseph Ekandem Y1 - 2015/09/26 PY - 2015 N1 - https://doi.org/10.11648/j.ajbio.20150306.11 DO - 10.11648/j.ajbio.20150306.11 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 197 EP - 202 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20150306.11 AB - Douvir–N is a combination of lamivudine, zidovudine and nevirapine used for the treatment of patients with Human Immunodeficiency Virus. The objective of this study was to investigate the effect of combined administration of Douvir–N and folic acid on the histology and some Biochemical parameters in the liver of Wistar rats. Forty adult albino Wistar rats were randomly divided into four groups of ten animals each. Group A served as control and were administered with 1ml of distilled water, group B animals were administered with 9.29mg/kg body weight of Douvir–N, Group C animals were administered with a combination of 9.29mg/kg of Douvir–N and 0.07mg/kg of folic acid. Animals in group D were administered with 0.07mg/kg of folic acid. Animals were sacrificed after 30 days and dissected. The liver was removed and fixed in 10% buffered formaldehyde, processed and stained using Haematoxylin and Eosin staining method, carcino-embryonic antigen (CEA) and cytokeratin-7 (CK-7) immunochemistry methods. Stained slides were viewed using light microscope. Blood samples from each rat was collected using syringes and needles, The sera were extracted into fresh test tubes and stored in a refrigerator for analysis of aspartate aminotransaminase test (AST), alanine aminotransaminase test (ALT), alkaline phosphotase (ALP). The liver of Wistar rats administered with Douvir–N showed distortions in the liver with moderate dilatation of the sinusoidal spaces and nuclei pyknotic changes, with increased expression of CEA and CK7 in the groups treated with Douvir–N than the control groups. There was a significant increase in ALP in the Douvir–N groups. These changes were ameliorated when Douvir–N was combined with Folic acid. The findings suggest that Douvir–N can distort the cytoarchitecture and Biochemical parameters of the liver which could be ameliorated by co-administration with folic acid. Folic acid should be given as adjuvant drug to patients on Douvir–N therapy. VL - 3 IS - 6 ER -
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