The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine

Ayoub R. Aldalou; Ismail Abdel-Aziz; Majed M. Hania; Osama Shahwan; Al Monzer Al–Hamidi

doi:doi:10.11648/j.ajbls.20160404.11

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The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine

Ayoub R. Aldalou, Ismail Abdel-Aziz, Majed M. Hania, Osama Shahwan, Al Monzer Al–Hamidi

Published in American Journal of Biomedical and Life Sciences (Volume 4, Issue 4)

Received: 10 May 2016 Accepted: 12 June 2016 Published: 4 July 2016

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Abstract

The aim of this study was to determine the level of toxicity of nitrosamine on some biochemical and hematological parameters and the curative effect of olive oil and ephedra extract on domestic 24 male rabbits divided into four groups, each with different doses which were applied for 33 days. In the present study, olive oil and ephedra administration with nitrosamine reduced its induced harm in a significant way. These finding suggest that olive oil, effectively, reduces nitrosamine-induced toxicity more than ephedra, and generally, their use concomitantly with nitrosamine have an extremely beneficial role in overcoming the adverse effects occurring due to chronic ingestion of nitrosamines.

Published in	American Journal of Biomedical and Life Sciences (Volume 4, Issue 4)
DOI	10.11648/j.ajbls.20160404.11
Page(s)	54-60
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Nitrosamine, Olive Oil, Ephedra, Biochemical and Hematological

References

[1]	Ashley, D. L., O’Connor, R. J., Bernert et al, J. T. Effect of difer-ing levels of tobacco-speciic nitrosamines in cigarette smoke on the levels of biomarkers in smokers,” Cancer Epidemiology Biomarkers and Prevention, vol. 19, no. 6, pp. 1389–1398, 2010.
[2]	Clapp, R., Jacobs, M., and Lijinsky, W. N -nitroso compounds,” in Patty’s Toxicology, pp. 401–432, Wiley, 2012.
[3]	Lijinsky, W. N-nitroso - compounds in the diet, Mutation Research. Genetic Toxicology and Environmental Mutagenesis, vol. 443, no. 1-2, pp. 129–138, 1999.
[4]	Tricker, A. R. N-nitroso compounds and man: sources of expo-sure, endogenous formation and occurrence in body luids, European Journal of Cancer Prevention, vol. 6, no. 3, pp. 226–268, 1997.
[5]	Ziebarth, D., and Teichmann, B., Nitrosation of orally adminis-tered drugs under simulated stomach conditions, IARC Scien-tiic Publication, no. 31, pp. 231–244, 1980.
[6]	Preussmann, R. Occurrence and exposure to N-nitroso com-pounds and precursors, IARC scientiic publications, no. 57, pp. 3–15, 1984.
[7]	Sakai, A., Inoue, T., and Tanimura, A. Formation of volatile nitrosamines by drug-nitrite interactions under physiological conditions, Gann, vol. 75, no. 3, pp. 245–252, 1984.
[8]	Gillatt, P. N., Palmer, R. C., Smith, P. L., Walters, C. L., and Reed, P. I. Susceptibilities of drugs to nitrosation under simulated gastric conditions, Food and Chemical Toxicology, vol. 23, no. 9, pp. 849–855, 1985.
[9]	Ohta, T., Asabe, Y., and Takitani, S. Identiication of mutagenic nitrosation products of antipyrine and evaluation of their formation under model stomach conditions, Chemical and Pharmaceutical Bulletin, vol. 34, no. 9, pp. 3866–3872, 1986.
[10]	Ziebarth, D., Schramm, T., and Toppel, A. Drugs from the classes of tricyclic antidepressives and antiepileptics, nitrosa-table under simulated human gastric conditions, Archiv fur Geschwulstforschung, vol. 59, no. 4, pp. 257–263, 1989.
[11]	Brambilla G., and Martelli, A. Genotoxic and carcinogenic risk to humans of drug-nitrite interaction products, Mutation Research—Reviews in Mutation Research, vol. 635, no. 1, pp. 17–52, 2007.
[12]	Brender, J. D., Werler, M. M., Shinde M. U., et al., Nitrosatable drug exposure during the irst trimester of pregnancy and selected congenital malformations, Birth Defects Research A, vol. 94, no. 9, pp. 701–713, 2012.
[13]	Bochert, G., Platzek, T., Blankenburg, G., Wiessler, M., and Neubert, D. Embryotoxicity induced by alkylating agents: let-sided preponderance of paw malformations induced by acetoxymethyl-methylnitrosamine in mice, Archives of Toxicol-ogy, vol. 56, no. 3, pp. 139–150, 1985.
[14]	Fort, D. J., Rayburn, J. R., De Young D. J., and Bantle, J. A. Assessing the eicacy of an Aroclor 1254-induced exogenous metabolic activation system for FETAX, Drug and Chemical Toxicology, vol. 14, no. 1-2, pp. 143–160, 1991.
[15]	Sheweita, S. A., Baghdadi, H., and Allam, A. R. Roleofgenetic changes in the progression of cardiovascular diseases, International Journal of Biomedical Science, vol. 7, no. 4, pp. 238–248, 2011.
[16]	Astrup, A., Breum, L., Toubro, S., et al. The effect and safety of an ephedrine/caffeine compound compared to ephedrine, caffeine and placebo in obese subjects on an energy restricted diet. A double-blind trial. Int J Obes Relat Metab Disord. 16: 269-277; 1992
[17]	Ben, t S., Tiedt, T. N., Odden, M. C., et al. The relative safety of ephedra compared with other herbal products. Ann Intern Med. 138: 468-47; 12003.
[18]	Breum, L., Pedersen, J. K., Ahlstrom, F., et al. Comparison of an ephedrine/caffeine combination and dexfenfluramine in the treatment of obesity. A double-blind multi-centre trial in general practice. Int J Obes Relat Metab Disord. 18: 99-103; 1994.
[19]	Molnar, D., Torok, K., Erhardt, E., et al. Safety and efficacy of treatment with an ephedrine/caffeine mixture. The first double-blind placebo-controlled pilot study in adolescents. Int J Obes Relat Metab Disord. 24: 1573-1578; 2000.
[20]	Astrup, A., Breum, L., Toubro, S. Pharmacological and clinical studies of ephedrine and other thermogenic agonists. Obes Res. 3: 537S-540S; 1995.
[21]	Decker, E. A. The role of phenolics, conjugated linoleic acid, carnosine, and pyrroloquinoline quinone as non essential dietary antioxidants. Nutr Rev 53: 49-58; 1995.
[22]	Caruso, D., Berra, B., Giavarini, F., Cortesi, N., Fedeli, E., Galli, G. Effect of Virgin Olive Oil Phenolic Compounds on in Vitro Oxidation of Human Low Density Lipoproteins. Nutr. Metab. Cardiovasc. Dis. 9, 102-107, 1999.
[23]	Visioli, F., Bellomo, G., Montedoro, G., Galli, C. Low Density Lipoprotein Oxidation Is Inhibited in Vitro by Olive Oil Constituents. Atherosclerosis. 117, 25-32, 1995.
[24]	Choi, S. Y., Chung, M. J., Sung, N. J. Volatile N-nitrosamine inhibition after intake of Korean green tea and Maesil (Prunus mume SIEB. et ZACC.) extracts with an amine-rich diet in subjects ingesting nitrate. Food Chem Toxicol. 40: 949-957; 2002
[25]	Aruoma, O. I. Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc. 75: 199-212; 1998.
[26]	Wiechetek, M., Garwacki, S., Karlik, W., Lewicki, J., Souffrant, W. Effect of nitrite on ureagenesis and carbohydrate metabolism in isolated rat hepatocytes. Arch of Environ Contamin Toxicol. 24: 375-380; 1992.
[27]	Anil, K. B., Manju, B., Giridhar, S., Deepak, B. Protective role of Vitamin E pre-treatment on N-nitrosodiethylamine induced oxidative stress in rat liver. Chem Biol Interact. 20: 101-102; 2005.
[28]	Liu, C. T., Hse, H., Lii, C. K., Chen, P. S., Sheen, L. Y. Effects of garlic oil and diallyl trisulfide on glycemic control in diabetic rats. Eur J Pharmacol. 516: 165-173; 2005.
[29]	Eidi, A., Eidi, M., Esmaeili, E. Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin induced diabetic rats. Phytomedicine. 13: 624-629; 2006.
[30]	Zurovsky, Y., Haber, C. Antioxidants attenuate endotoxin-generation induced acute renal failure in rats. Scand J Urol Nephrol. 29: 147-154; 1995.
[31]	Hassan, H. A., and Fattoh A. M. Evaluation of free radical-scavenging and antioxidant properties of black berry ag ainst fluoride toxicity in rabbits. Food Chem. Toxicol., 48: 1999; 2004.
[32]	Eremin, Y. N., Yocharina, M. G. Effect of nitrites on the state of thyroid gland in iodine deficiency and different diets. Vopr Pitan. 5: 60-62; 1981.
[33]	Kolpakov, V., Gordon, D., Kulik, T. J. Nitric oxide-generating compounds inhibit total protein and collagen synthesis in cultured vascular smooth muscle cells. Circ Res. 76: 305-309; 1995.
[34]	Kalantari, H., Salehi, M. The protective effect of garlic oil on hepatotoxicity induced by acetaminophen in mice and comparison with N-acetylcysteine. Saudi Med J. 22: 1080-4; 2001.
[35]	Hikino, H., Tohkin, M., Kiso, Y., Namiki, T., Nishimura, S., Takeyama, K. Antihepatotoxic Actions of Allium sativum Bulbs 1. Planta Med. 52: 163-168; 1986.
[36]	Andraws, R., Chawla, P., Brown, D. L. Cardiovascular effects of ephedra alkaloids: a comprehensive review. Prog Cardiovasc Dis. 47 (4): 217-225; 2005.
[37]	Greenway, F. L. The safety and efficacy of pharmaceutical and herbal caffeine and ephedrine use as a weight loss agent. Obes Rev. 2 (3): 199-211; 2001.
[38]	Savita Mishra, Vidhu Aeri and Deepshikha Pande KatareHepatoprotective medication for liver injury. World J. of Pharm. and Pharmaceutical Sciences, 3 (5), 891-932; 2014.
[39]	Abdel-Aziz, I. Hematological and biochemical study on rabbits post whole-body X-irradiation and treatment by Nigella Sativa oil or olive oil. J. pest Cont. E. Sci. 8 (1): 65-84; 2000.
[40]	Galea, V., Preda, N., Popa, L., and Simu, U. Experimental production of nitrosamines in vivo. IARC. Sci. pub. 2: 121; 1995.

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Ayoub R. Aldalou, Ismail Abdel-Aziz, Majed M. Hania, Osama Shahwan, Al Monzer Al–Hamidi. (2016). The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine. American Journal of Biomedical and Life Sciences, 4(4), 54-60. https://doi.org/10.11648/j.ajbls.20160404.11

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ACS Style

Ayoub R. Aldalou; Ismail Abdel-Aziz; Majed M. Hania; Osama Shahwan; Al Monzer Al–Hamidi. The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine. Am. J. Biomed. Life Sci. 2016, 4(4), 54-60. doi: 10.11648/j.ajbls.20160404.11

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AMA Style

Ayoub R. Aldalou, Ismail Abdel-Aziz, Majed M. Hania, Osama Shahwan, Al Monzer Al–Hamidi. The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine. Am J Biomed Life Sci. 2016;4(4):54-60. doi: 10.11648/j.ajbls.20160404.11

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@article{10.11648/j.ajbls.20160404.11,
  author = {Ayoub R. Aldalou and Ismail Abdel-Aziz and Majed M. Hania and Osama Shahwan and Al Monzer Al–Hamidi},
  title = {The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {4},
  pages = {54-60},
  doi = {10.11648/j.ajbls.20160404.11},
  url = {https://doi.org/10.11648/j.ajbls.20160404.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160404.11},
  abstract = {The aim of this study was to determine the level of toxicity of nitrosamine on some biochemical and hematological parameters and the curative effect of olive oil and ephedra extract on domestic 24 male rabbits divided into four groups, each with different doses which were applied for 33 days. In the present study, olive oil and ephedra administration with nitrosamine reduced its induced harm in a significant way. These finding suggest that olive oil, effectively, reduces nitrosamine-induced toxicity more than ephedra, and generally, their use concomitantly with nitrosamine have an extremely beneficial role in overcoming the adverse effects occurring due to chronic ingestion of nitrosamines.},
 year = {2016}
}

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TY  - JOUR
T1  - The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine
AU  - Ayoub R. Aldalou
AU  - Ismail Abdel-Aziz
AU  - Majed M. Hania
AU  - Osama Shahwan
AU  - Al Monzer Al–Hamidi
Y1  - 2016/07/04
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20160404.11
DO  - 10.11648/j.ajbls.20160404.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 54
EP  - 60
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20160404.11
AB  - The aim of this study was to determine the level of toxicity of nitrosamine on some biochemical and hematological parameters and the curative effect of olive oil and ephedra extract on domestic 24 male rabbits divided into four groups, each with different doses which were applied for 33 days. In the present study, olive oil and ephedra administration with nitrosamine reduced its induced harm in a significant way. These finding suggest that olive oil, effectively, reduces nitrosamine-induced toxicity more than ephedra, and generally, their use concomitantly with nitrosamine have an extremely beneficial role in overcoming the adverse effects occurring due to chronic ingestion of nitrosamines.
VL  - 4
IS  - 4
ER  -

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Author Information

Ayoub R. Aldalou

Department of Biochemistry, Faculty of Applied Sciences, Al-Aqsa University, Gaza, Palestine
Ismail Abdel-Aziz

Department of Biology, Faculty of Science, Islamic University of Gaza, Gaza, Palestine
Majed M. Hania

Department of Chemistry, Faculty of Science, Islamic University of Gaza, Gaza, Palestine
Osama Shahwan

Al Manar Lab – Khanyounis, Gaza Strip, Palestine
Al Monzer Al–Hamidi

Department of Biology, Faculty of Science, Islamic University of Gaza, Gaza, Palestine

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Plain Text BibTeX RIS

APA Style

Ayoub R. Aldalou, Ismail Abdel-Aziz, Majed M. Hania, Osama Shahwan, Al Monzer Al–Hamidi. (2016). The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine. American Journal of Biomedical and Life Sciences, 4(4), 54-60. https://doi.org/10.11648/j.ajbls.20160404.11

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ACS Style

Ayoub R. Aldalou; Ismail Abdel-Aziz; Majed M. Hania; Osama Shahwan; Al Monzer Al–Hamidi. The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine. Am. J. Biomed. Life Sci. 2016, 4(4), 54-60. doi: 10.11648/j.ajbls.20160404.11

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AMA Style

Ayoub R. Aldalou, Ismail Abdel-Aziz, Majed M. Hania, Osama Shahwan, Al Monzer Al–Hamidi. The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine. Am J Biomed Life Sci. 2016;4(4):54-60. doi: 10.11648/j.ajbls.20160404.11

Copy | Download

@article{10.11648/j.ajbls.20160404.11,
  author = {Ayoub R. Aldalou and Ismail Abdel-Aziz and Majed M. Hania and Osama Shahwan and Al Monzer Al–Hamidi},
  title = {The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {4},
  number = {4},
  pages = {54-60},
  doi = {10.11648/j.ajbls.20160404.11},
  url = {https://doi.org/10.11648/j.ajbls.20160404.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160404.11},
  abstract = {The aim of this study was to determine the level of toxicity of nitrosamine on some biochemical and hematological parameters and the curative effect of olive oil and ephedra extract on domestic 24 male rabbits divided into four groups, each with different doses which were applied for 33 days. In the present study, olive oil and ephedra administration with nitrosamine reduced its induced harm in a significant way. These finding suggest that olive oil, effectively, reduces nitrosamine-induced toxicity more than ephedra, and generally, their use concomitantly with nitrosamine have an extremely beneficial role in overcoming the adverse effects occurring due to chronic ingestion of nitrosamines.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - The Curative Effect of Olive Oil and Ephedra Extract on Some Biochemical and Haematological Parameters of Domestic Male Rabbits After Administration of Nitrosamine
AU  - Ayoub R. Aldalou
AU  - Ismail Abdel-Aziz
AU  - Majed M. Hania
AU  - Osama Shahwan
AU  - Al Monzer Al–Hamidi
Y1  - 2016/07/04
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20160404.11
DO  - 10.11648/j.ajbls.20160404.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 54
EP  - 60
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20160404.11
AB  - The aim of this study was to determine the level of toxicity of nitrosamine on some biochemical and hematological parameters and the curative effect of olive oil and ephedra extract on domestic 24 male rabbits divided into four groups, each with different doses which were applied for 33 days. In the present study, olive oil and ephedra administration with nitrosamine reduced its induced harm in a significant way. These finding suggest that olive oil, effectively, reduces nitrosamine-induced toxicity more than ephedra, and generally, their use concomitantly with nitrosamine have an extremely beneficial role in overcoming the adverse effects occurring due to chronic ingestion of nitrosamines.
VL  - 4
IS  - 4
ER  -

Copy | Download