Phenotypic Characters of <i>Staphylococcus Aureus</i> Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria

Abdulhadi Sale Kumurya; Hamza Sule; Nasidi Abba Ahmed

doi:doi:10.11648/j.ajim.20170505.12

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Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria

Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed

Published in American Journal of Internal Medicine (Volume 5, Issue 5)

Received: 19 April 2017 Accepted: 26 May 2017 Published: 11 August 2017

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Abstract

Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.

Published in	American Journal of Internal Medicine (Volume 5, Issue 5)
DOI	10.11648/j.ajim.20170505.12
Page(s)	74-78
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Staphylococcus Aureus, Biochemical, Characteristics, Fermentation, Identification

References

[1]	Ako-Nai, A. K, Ogunniyi, A. D., Lamikanra, A. B. and Torimiro, S. A. (1991). The Characterization of cilinical isolates of Staphylococcus Aureus in lie-Ife, Nigeria. Journal of Medical microbiology 32: 109-112.
[2]	Ako-Nai, A. K., Lamikanra, A. B. and Onipede, A. O. (1995). Incidence of pathogenic Microorganism in clinical specimens from hospital in South-western Nigeria. East African Medical Journal 72(7): 436-441.
[3]	Bello, C. S. S. and Qahtani, A. (2006). Pitfalls in the routine diagnosis of Staphylococcus Aureus. African Journal of Biotechnolgy. 4(1): 83-86.
[4]	Boyce, J. M. (1997). Epidemiology and prevention of nosocomial infections. In: The Staphylococci in human disease. Edited by Crosslay KB and Archer AL Churchill Livingston, New York. 309-329.
[5]	Casgrove, S. E., Sakoulas, G., Pemcevich E. N., Schwabar, M. J., Karchmer, A. W. and Carmeli. Y. (2003). Comparison of mortality associated with methicillin resistant and methicillin sensitive Staphylococcus Aureus bacteriemia: a meta analysis. Clinical Infectious Diseases. 36: 53-59.
[6]	Cheesbrough M (2010). District Laboratory Practice in Tropical Countries part two, New York, Cambridge University Press U.S. pp. 64-67.
[7]	Duguid, J. P. (1989). Cluster-forming Gram-positive cocci. In: Mackie and McCartney Practical Medical Microbiology, 13^th edn, Edited by J. G. Colle.
[8]	Emmanuel, O. N. and Magaji, S. N. (2011). Antibiotic susceptibility pattern of Staphylococcus Aureus from Clinical Isolates in tertiary insititution in northern Nigeria. Pan African Medical Journal. 8: 4.
[9]	Hass, D and Defago, G. (2005). Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbial. 3(4): 369–372.
[10]	Isenberg, H. D. (1998). Agar screening test to detect oxacillin (methicillin) resistant Staphylococcus Spp. In: Essential procedures for clinical microbiology. 4: 232-234.
[11]	Jayaratne, P. and Rutherford, C. (1999). Detection of methicillin resistant (MRSA) from growth on mannitol salt oxacillin agar using PCR for nosocomial surveillance. Diagnostic Microbioogy and Infectious Diseases. 35: 13–18.
[12]	Kloos, W. E. and Bannerman, T. L. (1994). Update on clinical significance of coagulase–negative Staphylococci. Clinical Microbiology Reviews. 7: 117-140.
[13]	Kumurya, A. S. (2015). Characters and Antibiotic Susceptibility Pattern of Typical and Atypical Staphylococcus Aureus Isolates from Clinical Samples in Northwestern Nigeria. Acta Velit. 1(3): 42-49. Available at www.actavelit.com.
[14]	Lowy, F. D. (2003). Antimicrobial resistance: the example of Staphylococcus Aureus. Journal of Clinical Investigations. 111: 1265-1273.
[15]	Mugalu, J. (2006). "Aetiology, risk factors and immediate outcome of bacteriologically confirmed neonatal septicaemia in Mulago hospital, Uganda." African Health Sciences 6(2): 120-126.
[16]	Najjuka, F. C., Kateete, D. P., Kimani, C. N., Katabazi, F. C., Okeng, A., Okee, M. S., Nanteza, A. and Joloba, M. L. (2010). Identification of: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobial, 9(23): 1186-1176.
[17]	Ndip, R. N., Ebah, L. M. E. and Onile, B. A. 1997). Antibiogram of Staphylococcus Aureus from clinical Syndromes in Ilorin, Nigeria. Journal of Medical Laboratry Science. 6: 24-26.
[18]	Nneoma, C. J. S. and Walter C. J. (2013). Conventional and Rapid Methods for Identification of Staphylococcus Aureus from Clinical Specimens. American Journal of Biomedical and Life Sciences. 1(3): 41-43.
[19]	Oni, A. A., Bakare, R. A., Okesola, A. O., Ogunlowo, H. A. and Ewete, A. F. (1997). Partern of bacterial pathogens in surgical wounds infections. African Journal Medical Sciences. 26: 139-140.
[20]	Plata, K., Rosato, A. E. and Wegrzyn, G. (2009). Staphylococcus Aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity. Acta Biochimica Polonica. 56: 597–612.
[21]	Shopsin, B. and Kreiswirth, B. N. (2003). Molecular epidemiology of Methicillin-resistant Staphylococcus Aureus. Emerging Infections Disease 7: 323-326.
[22]	Simor, A., Goodfellow, J., Loiue, L. and Loiue, M. (2001). Evaluation of a new medium, oxacillin resistance screening agar base, for the detection of methicillin-resistant from clinical specimens. Journal of Clinical Microbiology. 33: 3422.
[23]	Subhankari, P. C., Santanu, K. M., Somenath, R. (2011). Asian Pacific Journal of Tropical Medicine. 1(3): 212–216.
[24]	Unachukwu, C. N., Obunge, O. K. and Odia, O. J. (2005). The bacteriology of diabetic foot ulcer in Port Harcourt, Nigeria. Nigerian Journal of Medicine. 14(2): 173-176./.
[25]	Vlok, W. A. (1982). Essentials of medical microbiology. 2nd. J. B. Lippincott Philadelphia. 348-355.
[26]	Zadik, P. M., Davies, S., Whittaker, S. and Mason, C. (2001). Evaluation of a new selective medium for methicillin resistant Staphylococcus Aureus. Journal of Medical Microbiology. 50: 476–479.

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APA Style

Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed. (2017). Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. American Journal of Internal Medicine, 5(5), 74-78. https://doi.org/10.11648/j.ajim.20170505.12

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ACS Style

Abdulhadi Sale Kumurya; Hamza Sule; Nasidi Abba Ahmed. Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. Am. J. Intern. Med. 2017, 5(5), 74-78. doi: 10.11648/j.ajim.20170505.12

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AMA Style

Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed. Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. Am J Intern Med. 2017;5(5):74-78. doi: 10.11648/j.ajim.20170505.12

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@article{10.11648/j.ajim.20170505.12,
  author = {Abdulhadi Sale Kumurya and Hamza Sule and Nasidi Abba Ahmed},
  title = {Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {5},
  pages = {74-78},
  doi = {10.11648/j.ajim.20170505.12},
  url = {https://doi.org/10.11648/j.ajim.20170505.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.12},
  abstract = {Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.},
 year = {2017}
}

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TY  - JOUR
T1  - Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria
AU  - Abdulhadi Sale Kumurya
AU  - Hamza Sule
AU  - Nasidi Abba Ahmed
Y1  - 2017/08/11
PY  - 2017
N1  - https://doi.org/10.11648/j.ajim.20170505.12
DO  - 10.11648/j.ajim.20170505.12
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 74
EP  - 78
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20170505.12
AB  - Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.
VL  - 5
IS  - 5
ER  -

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Author Information

Abdulhadi Sale Kumurya

Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
Hamza Sule

Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria
Nasidi Abba Ahmed

Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University, Kano, Nigeria

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APA Style

Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed. (2017). Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. American Journal of Internal Medicine, 5(5), 74-78. https://doi.org/10.11648/j.ajim.20170505.12

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ACS Style

Abdulhadi Sale Kumurya; Hamza Sule; Nasidi Abba Ahmed. Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. Am. J. Intern. Med. 2017, 5(5), 74-78. doi: 10.11648/j.ajim.20170505.12

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AMA Style

Abdulhadi Sale Kumurya, Hamza Sule, Nasidi Abba Ahmed. Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria. Am J Intern Med. 2017;5(5):74-78. doi: 10.11648/j.ajim.20170505.12

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@article{10.11648/j.ajim.20170505.12,
  author = {Abdulhadi Sale Kumurya and Hamza Sule and Nasidi Abba Ahmed},
  title = {Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {5},
  pages = {74-78},
  doi = {10.11648/j.ajim.20170505.12},
  url = {https://doi.org/10.11648/j.ajim.20170505.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170505.12},
  abstract = {Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.},
 year = {2017}
}

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TY  - JOUR
T1  - Phenotypic Characters of Staphylococcus Aureus Isolates from Clinical Samples in Aminu Kano Teaching Hospital, Kano, Nigeria
AU  - Abdulhadi Sale Kumurya
AU  - Hamza Sule
AU  - Nasidi Abba Ahmed
Y1  - 2017/08/11
PY  - 2017
N1  - https://doi.org/10.11648/j.ajim.20170505.12
DO  - 10.11648/j.ajim.20170505.12
T2  - American Journal of Internal Medicine
JF  - American Journal of Internal Medicine
JO  - American Journal of Internal Medicine
SP  - 74
EP  - 78
PB  - Science Publishing Group
SN  - 2330-4324
UR  - https://doi.org/10.11648/j.ajim.20170505.12
AB  - Bacterial colonies can differ greatly in their morphologies. These differences can help us in identifying different species of bacteria. The clinical isolates of Staphylococcus Aureus are subjected to standard biochemical tests, to observe the biochemical characteristics. One hundred and ten (110) pathogenic Staphylococcus Aureus strains were used in this study. Characteristics of these strains were determined by biochemical tests such as catalase, coagulase, DNase test, test for beta haemolysin, fermentation of mannitol and lactose. Staphylococcus Aureus [American Type Culture Collection (ATCC) 25923] was used as a reference control organism. From this study, males were more infected than females, having 60 (61.8%) and 42 (38.2%) respectively. The age group with the highest number of isolates was (0-10) years while blood culture had the highest frequency of Staphylococcus Aureus isolates with a frequency of 42 (38.1%). It was also observed that 100% of Staphylococcus Aureus showed positive results in catalase and tube coagulase, about 101 (91.8%) were positive for slide coagulase production test, 102 (92.7%) strains were positive to DNase test, 63 (57.3%) were positive to beta haemolysin while 79 (71.8%) and 106 (96.4%) strains fermented Mannitol and lactose respectively. This study reveals that no single test that can be used for the identification of Staphylococcus Aureus, hence the need for sequel testing using proper biochemical tests should be used for the identification of Staphylococcus Aureus isolates.
VL  - 5
IS  - 5
ER  -

Copy | Download