Endophytic bacterial diversity in sugarcane plant cultivated on Latosols and Acrisols of the Eastern of South Vietnam was studied. Sugarcane plant samples were taken in four sites (districts/towns) (Xuanloc, Dinhquan, Vinhc uu and Trangbom) of Dong Nai province. Endophytic bacteria were isolated in LGI medium together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 27 isolates were isolated on LGI medium and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 10 isolates having the best characteristics. The sequences from selected endophytic bacteria (10 isolates) showed high degrees of similarity to those of the GenBank references strains (between 98% and 99%). All of them are Proteobacteria (gram-negative bacteria) and 3 isolates belonged to Alpha-proteobacteria (30%), 2 isolates were Beta-proteobacteria (20%), 5 isolates were Gamma-proteobacteria (50%). Based on Pi value (nucleotide diversity), Gamma-proteobacteria group had the highest theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Gamma-proteobacteria group had the highest values in comparison of two groups. From these results showed that Enterobacter oryzae LT7, Achromobacter xylosoxidans T16, Achromobacter insolitus R15b and Pantoea agglomerans T12 revealed promising candidates with multiple beneficial characteristics and they have the potential for application as inoculants or bio-fertilizer adapted to poor latosols and acrisols because they are not only famous strains but also are safety strains for sustainable agriculture.
| Published in | American Journal of Life Sciences (Volume 2, Issue 6) |
| DOI | 10.11648/j.ajls.20140206.16 |
| Page(s) | 361-368 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Acrisols, 16S rRNA Gene Sequence, Latosols, the Eastern of the South Vietnam, Endophytic Bacteria, Sugarcane
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APA Style
Hoang Minh Tam, Cao Ngoc Diep. (2014). Isolation, Characterization and Identification of Endophytic Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Soils of the Dong Nai Province, Southeast of Vietnam. American Journal of Life Sciences, 2(6), 361-368. https://doi.org/10.11648/j.ajls.20140206.16
ACS Style
Hoang Minh Tam; Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Soils of the Dong Nai Province, Southeast of Vietnam. Am. J. Life Sci. 2014, 2(6), 361-368. doi: 10.11648/j.ajls.20140206.16
AMA Style
Hoang Minh Tam, Cao Ngoc Diep. Isolation, Characterization and Identification of Endophytic Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Soils of the Dong Nai Province, Southeast of Vietnam. Am J Life Sci. 2014;2(6):361-368. doi: 10.11648/j.ajls.20140206.16
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Download@article{10.11648/j.ajls.20140206.16,
author = {Hoang Minh Tam and Cao Ngoc Diep},
title = {Isolation, Characterization and Identification of Endophytic Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Soils of the Dong Nai Province, Southeast of Vietnam},
journal = {American Journal of Life Sciences},
volume = {2},
number = {6},
pages = {361-368},
doi = {10.11648/j.ajls.20140206.16},
url = {https://doi.org/10.11648/j.ajls.20140206.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140206.16},
abstract = {Endophytic bacterial diversity in sugarcane plant cultivated on Latosols and Acrisols of the Eastern of South Vietnam was studied. Sugarcane plant samples were taken in four sites (districts/towns) (Xuanloc, Dinhquan, Vinhc uu and Trangbom) of Dong Nai province. Endophytic bacteria were isolated in LGI medium together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 27 isolates were isolated on LGI medium and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 10 isolates having the best characteristics. The sequences from selected endophytic bacteria (10 isolates) showed high degrees of similarity to those of the GenBank references strains (between 98% and 99%). All of them are Proteobacteria (gram-negative bacteria) and 3 isolates belonged to Alpha-proteobacteria (30%), 2 isolates were Beta-proteobacteria (20%), 5 isolates were Gamma-proteobacteria (50%). Based on Pi value (nucleotide diversity), Gamma-proteobacteria group had the highest theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Gamma-proteobacteria group had the highest values in comparison of two groups. From these results showed that Enterobacter oryzae LT7, Achromobacter xylosoxidans T16, Achromobacter insolitus R15b and Pantoea agglomerans T12 revealed promising candidates with multiple beneficial characteristics and they have the potential for application as inoculants or bio-fertilizer adapted to poor latosols and acrisols because they are not only famous strains but also are safety strains for sustainable agriculture.},
year = {2014}
}
TY - JOUR T1 - Isolation, Characterization and Identification of Endophytic Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Soils of the Dong Nai Province, Southeast of Vietnam AU - Hoang Minh Tam AU - Cao Ngoc Diep Y1 - 2014/12/18 PY - 2014 N1 - https://doi.org/10.11648/j.ajls.20140206.16 DO - 10.11648/j.ajls.20140206.16 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 361 EP - 368 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20140206.16 AB - Endophytic bacterial diversity in sugarcane plant cultivated on Latosols and Acrisols of the Eastern of South Vietnam was studied. Sugarcane plant samples were taken in four sites (districts/towns) (Xuanloc, Dinhquan, Vinhc uu and Trangbom) of Dong Nai province. Endophytic bacteria were isolated in LGI medium together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 27 isolates were isolated on LGI medium and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis but there were 10 isolates having the best characteristics. The sequences from selected endophytic bacteria (10 isolates) showed high degrees of similarity to those of the GenBank references strains (between 98% and 99%). All of them are Proteobacteria (gram-negative bacteria) and 3 isolates belonged to Alpha-proteobacteria (30%), 2 isolates were Beta-proteobacteria (20%), 5 isolates were Gamma-proteobacteria (50%). Based on Pi value (nucleotide diversity), Gamma-proteobacteria group had the highest theta values and Theta values (per sequence) from S of SNP for DNA polymorphism were calculated for each group and Gamma-proteobacteria group had the highest values in comparison of two groups. From these results showed that Enterobacter oryzae LT7, Achromobacter xylosoxidans T16, Achromobacter insolitus R15b and Pantoea agglomerans T12 revealed promising candidates with multiple beneficial characteristics and they have the potential for application as inoculants or bio-fertilizer adapted to poor latosols and acrisols because they are not only famous strains but also are safety strains for sustainable agriculture. VL - 2 IS - 6 ER -
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