Inhibitory Effect of Extracts from <i>Datura metel</i> Leaf on Mushroom Tyrosinase

David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo

doi:doi:10.11648/j.ajls.20160402.15

| Peer-Reviewed

Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo

Published in American Journal of Life Sciences (Volume 4, Issue 2)

Received: 5 February 2016 Accepted: 27 February 2016 Published: 6 May 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.

Published in	American Journal of Life Sciences (Volume 4, Issue 2)
DOI	10.11648/j.ajls.20160402.15
Page(s)	47-50
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Datura metel, Tyrosinase, Inhibition

References

[1]	Cowan MM. (1999). Plant products as Antimicrobial agents. Clinical Microbiology Reviews, 12 (4): 564-582.
[2]	Eloff JN. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Ethnopharmacology, 60: 1-8.
[3]	Aburjai T, Natsheh FM. (2003). Plants used in Cosmetics. Phototherapy Res., 17: 98-1000.
[4]	Momtaz S. (2006). Anti tyrosinase activity of eleven plants. Honours Thesis. Diseases, what’s new? European Academy of Dermatology and Venereology JEADV, 17: 663-669.
[5]	Nnoruka EN. (2005). Skin diseases in south-east Nigeria: A current perspective. International Journal of Dermatology, 44(1): 29-33.
[6]	Adodo A. (2005) New Frontiers in African Medicine. Pax Herbal clinic Nig. Ltd. Ewu Edo State. 199.
[7]	Idu M, Osemwegie OO, Odia EA, Onyibe HI. (2007). A survey of indigenous flora used by folk medicine practitioners in Yola council area of Adamawa State, Nigeria. Plant Archives, 7(2): 517-521.
[8]	Adodo A. (2003). The Healing Radiance of the Soul: A Guide to Holistic Healing. Agelex Publications, Nigeria. 171.
[9]	Lewis WH. (2003). Pharmaceutical discoveries based on ethnomedicinal plants: 1985-2000 and beyond. Economic Botany 57(17): 126-134.
[10]	Kirsten B. (1986). The Genus Datura: From Research subject to powerful Hallucinogen.
[11]	Conklin ME. (1976). Genetic and Biochemical Aspects of the development of Datura. Monographs in Development Biology. New York. Kerger.
[12]	Heiser CB. (1969). Nightshades, The paradoxical plants, San Francisco: WH freeman and co.
[13]	Avery AG, Satina S, Rietsema J. (1959). Blakeslee: The Genus Datura, New York, Roland Press Co.
[14]	Tijanni AA, Adeniyi DT, Adekomi DA. (2012). Datura metel is deleterious to the visual cortex of adult wistar rats. Advances in Applied Science Research, 3(2): 944-949.
[15]	Mukherjee PK, Badami S, Wahile AM, Rajan S, Suresh B. (2001). Evaluation of Tyrosinase inhibitory activity of some Indian spices. Journal of Natural remedies, 1/2; 125-129.
[16]	Baek S, Kim J, Kim D, Lee C, Kim J, Chung DK, Lee C. (2008). Inhibitory effect of dalbergioidin isolated from the trunk of Lespedeza cyrtobotrya on melanin biosynthesis. J. Microbiol. Biotechnol., 18: 874-879.
[17]	Donatus EO, Ephraim CI. (2009). Isolation, characterization and antibacterial activity of alkaloid from Datura metel Linn leaves. African Journal of Pharmacy and Pharmacology 3(5): 277–281.
[18]	Yussuf (1991). Phytochemical and antimicrobial studies. International Journal of Pharmacognosy 29: 252–258.
[19]	Hossain MA, Nagooru MR. (2011). Biochemical profiling and total flavonoids contents of leaves crude extract of endemic medicinal plant Corydyline terminalis L. Kunth. Pharmacognosy Journal 3(24): 25–29.
[20]	Suresh SN, Nagarajan N. (2009). Preliminary phytochemical and antimicrobial activity analysis of Begonia malabarica Lam. Journal of Basic & Applied Biology 3(1&2): 59–61.
[21]	Gonzalez-Guevara JL, Gonzalez-Lavaut JA, Pino-Rodriguez S, Garcia-Torres M, Carballo-Gonzalez MT, Echemendia-Arana OA, Molina-Torres J, Prieto-Gonzalez S. (2004). Phytochemical screening and in vitro antiherpetic activity of four Erythtroxylum species. Acta Farmaceut Bonaer 23(4): 506–509.

Cite This Article

Plain Text BibTeX RIS

APA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. (2016). Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. American Journal of Life Sciences, 4(2), 47-50. https://doi.org/10.11648/j.ajls.20160402.15

Copy | Download

ACS Style

David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am. J. Life Sci. 2016, 4(2), 47-50. doi: 10.11648/j.ajls.20160402.15

Copy | Download

AMA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am J Life Sci. 2016;4(2):47-50. doi: 10.11648/j.ajls.20160402.15

Copy | Download

@article{10.11648/j.ajls.20160402.15,
  author = {David Morakinyo Sanni and Oluwasegun Victor Omotoyinbo},
  title = {Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {2},
  pages = {47-50},
  doi = {10.11648/j.ajls.20160402.15},
  url = {https://doi.org/10.11648/j.ajls.20160402.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160402.15},
  abstract = {This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase
AU  - David Morakinyo Sanni
AU  - Oluwasegun Victor Omotoyinbo
Y1  - 2016/05/06
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160402.15
DO  - 10.11648/j.ajls.20160402.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 47
EP  - 50
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160402.15
AB  - This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.
VL  - 4
IS  - 2
ER  -

Copy | Download

Author Information

David Morakinyo Sanni

Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Oluwasegun Victor Omotoyinbo

Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. (2016). Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. American Journal of Life Sciences, 4(2), 47-50. https://doi.org/10.11648/j.ajls.20160402.15

Copy | Download

ACS Style

David Morakinyo Sanni; Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am. J. Life Sci. 2016, 4(2), 47-50. doi: 10.11648/j.ajls.20160402.15

Copy | Download

AMA Style

David Morakinyo Sanni, Oluwasegun Victor Omotoyinbo. Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am J Life Sci. 2016;4(2):47-50. doi: 10.11648/j.ajls.20160402.15

Copy | Download

@article{10.11648/j.ajls.20160402.15,
  author = {David Morakinyo Sanni and Oluwasegun Victor Omotoyinbo},
  title = {Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {2},
  pages = {47-50},
  doi = {10.11648/j.ajls.20160402.15},
  url = {https://doi.org/10.11648/j.ajls.20160402.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160402.15},
  abstract = {This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase
AU  - David Morakinyo Sanni
AU  - Oluwasegun Victor Omotoyinbo
Y1  - 2016/05/06
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160402.15
DO  - 10.11648/j.ajls.20160402.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 47
EP  - 50
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160402.15
AB  - This study evaluated the tyrosinase-inhibition activities of three extracts; methanol, acetone and dichloromethane of Datura metel leaves. The bioactive components were extracted and then evaluated for their inhibitory effect in vitro on mushroom tyrosinase, using a colorimetric procedure. The Methanolic extract of the plant leaf had the highest inhibition of tyrosinase conversion of substrate L-DOPA with 72.14% inhibition at peak concentration of 400 µg/ml considered unlike their respective acetone (51.08%) and dichloromethane (65.57%) extracts. All the Datura metel extracts had below 50% inhibition between concentrations of 3.1 µg/ml to 200 µg/ml except the methanolic extract which had 53.46% inhibition at 200 µg/ml. Although, there was a steady increase in tyrosinase inhibition for all plant extracts, however none of the plants extracts inhibition exceeded kojic acids inhibition at all concentrations considered.
VL  - 4
IS  - 2
ER  -

Copy | Download