HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.
| Published in | American Journal of Life Sciences (Volume 6, Issue 1) |
| DOI | 10.11648/j.ajls.20180601.12 |
| Page(s) | 7-12 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2018. Published by Science Publishing Group |
Nocodazole, F-actin, Microtubules, Growth Rate, HeLa Cells
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APA Style
Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou. (2018). A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. American Journal of Life Sciences, 6(1), 7-12. https://doi.org/10.11648/j.ajls.20180601.12
ACS Style
Jiayi Lu; Jorge De La Torre; Carson McCann; Maureen Madar; Qiongqiong Zhou. A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. Am. J. Life Sci. 2018, 6(1), 7-12. doi: 10.11648/j.ajls.20180601.12
AMA Style
Jiayi Lu, Jorge De La Torre, Carson McCann, Maureen Madar, Qiongqiong Zhou. A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure. Am J Life Sci. 2018;6(1):7-12. doi: 10.11648/j.ajls.20180601.12
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Download@article{10.11648/j.ajls.20180601.12,
author = {Jiayi Lu and Jorge De La Torre and Carson McCann and Maureen Madar and Qiongqiong Zhou},
title = {A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure},
journal = {American Journal of Life Sciences},
volume = {6},
number = {1},
pages = {7-12},
doi = {10.11648/j.ajls.20180601.12},
url = {https://doi.org/10.11648/j.ajls.20180601.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20180601.12},
abstract = {HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment.},
year = {2018}
}
TY - JOUR T1 - A Quantitative Study of Nocodazole’S Effect on HeLa Cells’ Growth Rate and F-actin Structure AU - Jiayi Lu AU - Jorge De La Torre AU - Carson McCann AU - Maureen Madar AU - Qiongqiong Zhou Y1 - 2018/03/09 PY - 2018 N1 - https://doi.org/10.11648/j.ajls.20180601.12 DO - 10.11648/j.ajls.20180601.12 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 7 EP - 12 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20180601.12 AB - HeLa cells were derived from human cervical cancer, which has been widely used as research model to study mammalian cell functions. In this work, nocodazole, a microtubule destabilizer, was used to treat HeLa cells with different concentrations. The results showed that nocodazole was able to inhibit HeLa cell’s growth rate significantly at the concentration of 100 nM. It suggested that nocodazole may inhibit cell growth through an alternative impacting effect other than destabilizing microtubules, since the effect of nocodazole destabilizing microtubule is usually not seen at micromolar range. However, at nanomolar concentration, nocodazole was not able to induce any changes in F-actin structure. Whereas, at concentration of 1 mM, nocodazole induced significant alterations of F-actin structure in HeLa cells. It indicated the strong relationship between microtubule and actin dynamics. Our work suggested that a molecule may exhibit different impacting mechanisms at different concentrations. Since nocodazole has been used as a chemotherapy reagent in cancer treatment, it will be beneficial to re-evaluate the effective concentration in terms of cancer treatment. VL - 6 IS - 1 ER -
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