Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot

Barakat F. M.; Abada K. A.; Abou-Zeid N. M.; El-Gammal Y. H. E.

doi:doi:10.11648/j.ajls.s.2014020602.12

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Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot

Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E.

Published in American Journal of Life Sciences (Volume 2, Issue 6-2)

Received: 20 August 2014 Accepted: 25 August 2014 Published: 5 September 2014

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Abstract

Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).

Published in	American Journal of Life Sciences (Volume 2, Issue 6-2) This article belongs to the Special Issue Role of Combination Between Bioagents and Solarization on Management of Crown-and Stem-Rot of Egyptian Clover
DOI	10.11648/j.ajls.s.2014020602.12
Page(s)	11-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Biological Control, Botrytis Fabae, Faba Bean Trichoderma Spp, Volatile and Non-Volatile Compounds

References

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Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E. (2014). Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. American Journal of Life Sciences, 2(6-2), 11-18. https://doi.org/10.11648/j.ajls.s.2014020602.12

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Barakat F. M.; Abada K. A.; Abou-Zeid N. M.; El-Gammal Y. H. E. Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. Am. J. Life Sci. 2014, 2(6-2), 11-18. doi: 10.11648/j.ajls.s.2014020602.12

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AMA Style

Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E. Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. Am J Life Sci. 2014;2(6-2):11-18. doi: 10.11648/j.ajls.s.2014020602.12

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@article{10.11648/j.ajls.s.2014020602.12,
  author = {Barakat F. M. and Abada K. A. and Abou-Zeid N. M. and El-Gammal Y. H. E.},
  title = {Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-2},
  pages = {11-18},
  doi = {10.11648/j.ajls.s.2014020602.12},
  url = {https://doi.org/10.11648/j.ajls.s.2014020602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020602.12},
  abstract = {Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).},
 year = {2014}
}

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TY  - JOUR
T1  - Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot
AU  - Barakat F. M.
AU  - Abada K. A.
AU  - Abou-Zeid N. M.
AU  - El-Gammal Y. H. E.
Y1  - 2014/09/05
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.s.2014020602.12
DO  - 10.11648/j.ajls.s.2014020602.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 11
EP  - 18
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2014020602.12
AB  - Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).
VL  - 2
IS  - 6-2
ER  -

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Author Information

Barakat F. M.

Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt
Abada K. A.

Plant Pathology Department, Faculty of Agriculture, Cairo University, Egypt
Abou-Zeid N. M.

Plant Pathology Research Institute, ARC. Giza, Egypt
El-Gammal Y. H. E.

Plant Pathology Research Institute, ARC. Giza, Egypt

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Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E. (2014). Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. American Journal of Life Sciences, 2(6-2), 11-18. https://doi.org/10.11648/j.ajls.s.2014020602.12

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Barakat F. M.; Abada K. A.; Abou-Zeid N. M.; El-Gammal Y. H. E. Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. Am. J. Life Sci. 2014, 2(6-2), 11-18. doi: 10.11648/j.ajls.s.2014020602.12

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AMA Style

Barakat F. M., Abada K. A., Abou-Zeid N. M., El-Gammal Y. H. E. Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot. Am J Life Sci. 2014;2(6-2):11-18. doi: 10.11648/j.ajls.s.2014020602.12

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@article{10.11648/j.ajls.s.2014020602.12,
  author = {Barakat F. M. and Abada K. A. and Abou-Zeid N. M. and El-Gammal Y. H. E.},
  title = {Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-2},
  pages = {11-18},
  doi = {10.11648/j.ajls.s.2014020602.12},
  url = {https://doi.org/10.11648/j.ajls.s.2014020602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020602.12},
  abstract = {Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Effect of Volatile and Non-Volatile Compounds of Trichoderma spp. on Botrytis Fabae the Causative Agent of Faba Bean Chocolate Spot
AU  - Barakat F. M.
AU  - Abada K. A.
AU  - Abou-Zeid N. M.
AU  - El-Gammal Y. H. E.
Y1  - 2014/09/05
PY  - 2014
N1  - https://doi.org/10.11648/j.ajls.s.2014020602.12
DO  - 10.11648/j.ajls.s.2014020602.12
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 11
EP  - 18
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2014020602.12
AB  - Antagonistic fungi naturally occurring on faba bean leaf surface were isolated and evaluated for their activity as bioagents for Botrytis fabae the causative agent of chocolate spot disease. Thirty isolates were purified and identified as 26 isolates of Trichoderma species (Trichoderma album, T. aureoviride , T. hamatum, T. harzianum and T. viride) and 4 isolates belonging to the genera of Cladosporium, Gliocladium, Epicoccum and Paecilomyces. The inhibitory effect of these isolates was assessed in vitro against the growth of B. fabae, which decreased it's mycelial growth on PDA plates. The inhibitory effect of Trichoderma spp. ranged between 51.11 - 77.78%. In addition, T. album (Isolate 2) gave the highest inhibition followed by T. harzianum (Isolate 6). Furthermore, under greenhouse conditions spraying of faba bean plants with any of Trichoderma spp. and Bio- Zeid as a biofungicide, 24 h before inoculation with B. fabae significantly reduced the severity of the disease after 14 days in the range of 3.0 - 4% compared with the control (8.7%). T. album (Isolate 2) was the highest antagonistic isolate (3.0%) followed by T. harzianum (Isolate 6) then T. hamatum (Isolate 6) and T. viride (Isolate 2), being 3.24, 3.30 and 3.40%, respectively. Volatile and non-volatile compounds produced by T. album (Isolate 2) exhibited the highest inhibition to the mycelial growth of B. fabae followed by T. harzianum (Isolate 6).
VL  - 2
IS  - 6-2
ER  -

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