Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.
| Published in | Animal and Veterinary Sciences (Volume 3, Issue 1) |
| DOI | 10.11648/j.avs.20150301.15 |
| Page(s) | 22-27 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Detection, Food Samples, PCR, Salmonella, Virulence Genes
| [1] | Alvarez, A. M. Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases. Ann. Rev. Phytopath. 42: 339-366. 2004. |
| [2] | Amini, K., Salehi, T. Z., Nikbakht, G., Ranjbar, R., Amini, J and Ashrafganjooei, S. B. Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from humans and animals in Iran. Afr. J. Microbiol. Res. 4: 2202 -2210. 2010. |
| [3] | Bennett, A. R., Greenwood, D., Tennant, C., Banks, J. G and Betts, R. P. Rapid and definitive detection of Salmonella in foods by PCR. Lett. Appl. Microbiol. 26:437-441. 1998. |
| [4] | Chiu, C. H., Wu, T. L., Su, L. H., Chu, C., Chia, J. H., Kuo, A, J., Chien, M. S and Lin, T. Y. The emergence in Taiwan of fluoroquinolone resistance in Salmonella enterica serotype Choleraesuis. N. Eng. J. Med. 346: 413-419. 2002. |
| [5] | Chiu, C.H. and Ou, J.T. Rapid identification of Salmonella serovars in faeces by specific detection of virulence genes, invA and spvC, by an enrichment broth-multiplex PCR combination assay. J. Clin. Microbiol. 34: 2619 -2622. 1996. |
| [6] | Cossi, M. V., Burin, R.C., Lopes, D.A., Dias, M. R., Castilho, N.P., de ArrudaPinto P. S. and Nero L. A. Antimicrobial resistance and virulence profiles of Salmonella isolated from butcher shops in Minas Gerais, Brasil. J. Food Prot. 76: 1633- 1637. 2013. |
| [7] | Darwin, K. H. and Miller V.L. Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clin. J. Microbiol. 12: 405-428. 1999. |
| [8] | Das, A., Hari, S. S., Shalini, U., Ganeshkumar, A. and Karthikeyan, M. Molecular Screening of Virulence Genes from Salmonella enterica Isolated from Commercial Food Stuffs. Biosciences Biotechnology Res. 9: 363-369. 2012 |
| [9] | Das, S., Singh, S., McClelland, M., Forst, S. and Gyaneshwar, P. Characterization of an Acid-Inducible Sulfatase in Salmonella enteric Serovar Typhimurium. Appl. Environ. Microbiol.79: 03707-03712. 2013. |
| [10] | Dione, M. M., Ikmapayi, U., Saha, D., Mohammed, I. N., Adegbola, A. R., Geerts, S., Leven, M and Antonio, M. Antimicrobial resistance and virulence genes of non-typhoidal Salmonella isolates in the Gambia and Senegal. J. Infect. Dev. Ctries. 5: 765-775. 2011. |
| [11] | Dorji, T.M., Mamata, G., Hyang-Mi, N., Chan, M.D., Suk-Chan. J. and Suk-Kyung, L. Antimicrobial susceptibility and virulence characteristics of Salmonella enteric Typhimurium isolates from healthy and diseased pigs in Korea. J. Food Prot. 9: 1481- 1486. 2014. |
| [12] | Ferretti, R., Mannazzu, L., Cocolin, L., Comi, G. and Clementi, F. Twelve-hour PCR-based method for detection of Salmonella spp in food. Appl. Environ. Microbiol. 74: 977-978. 2001. |
| [13] | Gorman, R., Bloomfield, S. and Adley, C. C. A study of cross-contamination of food-borne pathogens in the domestic kitchen in the Republic of Ireland. Int. J. Food Microbiol.76: 143-50. 2000. |
| [14] | Guo, L., Killfer, J., Kenny, P. B. and Amick-Morri, J. D. Use of arbitrarily primed Polymerase chain reaction to study Salmonella ecology in a turkey production. Environ. Poult Sc.78: 24-31. 1999. |
| [15] | Karmi, M. Detection of virulence genes (invA) in Salmonella isolated from Meat and Poultry Products. Int. J. Genetics 3: 7-12. 2013. |
| [16] | Li, Q., Skyberg, .J A., Fakhr, M. K., Sherwood, J.S., Molan, L.K. and Logue, C.M. Antimicrobial susceptibility and characterization of Salmonella isolates from processed Bison Carcasses. Appl Environ Microbiol 72: 3046- 3049. 2006. |
| [17] | Malorny, B., Hoorfar, J., Bunge, C. and Helmuth, R. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. App. Environ. Microbiol. 69: 290-296. 2003. |
| [18] | Mezal, E. H., Stefanova, R. and Khan, A.A. Isolation and molecular characterization of Salmonella enterica serovar Javiana from food, environmental and clinical samples. Int. J. Food Microbiol. 164: 113 -118. 2013. |
| [19] | Oluduro, A. O., Thonda, O.A. and Kolawole, D.O. Phenotypic and Molecular Characterization of Salmonella serotypes in cow milk and milk products in Nigeria. Afr. J. Biotechnology 13: 3774 -3789. 2014. |
| [20] | Pan, T. M. and Liu, Y. J. Identification of Salmonella enteritidis isolates by polymerase chain reaction and multiplex polymerase chain reaction. J. Microbiol. Immun. Infect. 35: 147-151. 2002. |
| [21] | Ramya, P., Madhavarao, T. and Rao, L. V. Study on the incidence of Salmonella enteritidis in poultry and meat samples by cultural and PCR methods. Vet. World5: 541-545. 2012. |
| [22] | Salehi, T. Z., Mahzounieh, M. and Saeedzadeh, A. Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method. Int. J. Poultry Sc. 4: 8, 557-559. 2005. |
| [23] | Schneder, A., Gronewald, C., Fandke, M., Kurth, B., Barkowski, S. and Berghof-ager, K. Real-time detection of the genus Salmonella with the Light Cycler system. Biochem. J. 4: 19-21. 2002. |
| [24] | Skyberg, J.A., Logue, C.M. and Nolan, L.K. Virulence genotyping of Salmonella spp with multiplex PCR. Avian Dis. 50: 77- 81. 2006. |
| [25] | Smith, S. 1., Opere, B., Fowora, M., Aderohunmu, A., Ibrahim, R., Omonigbehin, E., Bamidele, M. and Adeneye, A. Molecular characterization of Salmonella spp directly from snack and food commonly sold in Lagos, Nigeria. Southeast Asian J. Trop. Med. 43: 718-23. 2012. |
| [26] | Swamy, S.C., Barnhart. H., Lee, M.D. and Dreesen, D.W. Virulence determinants invA and spvC in salmonellae isolated from poultry products, wastewater and human sources. Appl. Environ. Microbiol. 62: 3768 -3771. 1996. |
| [27] | Todd, E. C. D., Greig, J. D., Bartleson, C. A and Michaels, B. S. Outbreaks where food workers have been implicated in the spread of foodborne disease. Part 4. Infective doses and pathogen carriage. J. Food Prot. 71: 2339-2373. 2008. |
| [28] | Van den Bogaard, A. E and Stobberingh, E. E. Epidemiology of resistance to antibiotics. Links between animals and humans. Int. J. Antimicrob. Agents14: 327-35. 2000. |
APA Style
Stella Ifeanyi Smith, Muinah Adenike Fowora, Adedamilola Atiba, Joseph Anejo-Okopi, Tina Fingesi, et al. (2015). Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Animal and Veterinary Sciences, 3(1), 22-27. https://doi.org/10.11648/j.avs.20150301.15
ACS Style
Stella Ifeanyi Smith; Muinah Adenike Fowora; Adedamilola Atiba; Joseph Anejo-Okopi; Tina Fingesi, et al. Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Anim. Vet. Sci. 2015, 3(1), 22-27. doi: 10.11648/j.avs.20150301.15
AMA Style
Stella Ifeanyi Smith, Muinah Adenike Fowora, Adedamilola Atiba, Joseph Anejo-Okopi, Tina Fingesi, et al. Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria. Anim Vet Sci. 2015;3(1):22-27. doi: 10.11648/j.avs.20150301.15
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.avs.20150301.15,
author = {Stella Ifeanyi Smith and Muinah Adenike Fowora and Adedamilola Atiba and Joseph Anejo-Okopi and Tina Fingesi and Mary Ehi Adamu and Emmanuel Adedayo Omonigbehin and Margaret Iteun Ugo-Ijeh and Moses Bamidele and Peter Odeigah},
title = {Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria},
journal = {Animal and Veterinary Sciences},
volume = {3},
number = {1},
pages = {22-27},
doi = {10.11648/j.avs.20150301.15},
url = {https://doi.org/10.11648/j.avs.20150301.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150301.15},
abstract = {Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected.},
year = {2015}
}
TY - JOUR T1 - Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria AU - Stella Ifeanyi Smith AU - Muinah Adenike Fowora AU - Adedamilola Atiba AU - Joseph Anejo-Okopi AU - Tina Fingesi AU - Mary Ehi Adamu AU - Emmanuel Adedayo Omonigbehin AU - Margaret Iteun Ugo-Ijeh AU - Moses Bamidele AU - Peter Odeigah Y1 - 2015/01/28 PY - 2015 N1 - https://doi.org/10.11648/j.avs.20150301.15 DO - 10.11648/j.avs.20150301.15 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 22 EP - 27 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20150301.15 AB - Food-borne salmonellosis is the most prevalent disease and major source of Salmonella spp in humans and its detection particularly in developing countries is quite cumbersome and time consuming. Molecular methods for its detection as well as the genotypic diversity of some of the genes responsible for Salmonella virulence are necessary. The aim of the study was to screen for Salmonella spp using the 16S rRNA, to determine whether the invA gene is specific for Salmonella detection as well as virulence genotyping of some genes present in Salmonella spp (invA, sitC and spvA, spvB and spvC) from food samples in Lagos, Nigeria. All 76 isolates tested positive for 16S rRNA gene while 53 (69.7%) were positive for salm3 and salm4 (389 bp) gene. PCR analysis of the invA gene (284bp) showed that 73 (96.1%) were positive, 38 (50%) of the isolates were positive for sitC gene while none were positive for spvA and spvB and with the multiplex –PCR of invA/spvC gene 25 (33%) were positive for invA (244 bp) gene and none positive for spvC gene. The use of invA gene for Salmonella detection in our food samples is recommended however for most of our isolates the virulence genes were not detected. VL - 3 IS - 1 ER -
Email: