Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites

Enedina Jiménez-Cardoso; Apolinar Cano-Estrada; Adrian-Cortes Campos; Leticia Eligio-García

doi:doi:10.11648/j.bio.20140202.12

| Peer-Reviewed

Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites

Enedina Jiménez-Cardoso, Apolinar Cano-Estrada, Adrian-Cortes Campos, Leticia Eligio-García

Published in American Journal of Bioscience and Bioengineering (Volume 2, Issue 2)

Received: 13 March 2014 Accepted: 17 July 2014 Published: 30 July 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The purpose of this study was to establish the optimal conditions to performance a multiple Polymerase Chain Reaction (multiplex) to detect the presence of emerging parasites Cryptosporidium spp., Mycrosporidium spp., Isospora belli, and Cyclospora spp. simultaneously, by targeting the 16S ribosomal gene in patients immunocompromised. After an exhaustive genetic analysis to obtain different primers sequences we tested the conditions of reagents concentration, time and temperature of reaction. The results showed the optimal conditions to make an efficient, specific, and reliable diagnostic method, performed in a simultaneous molecular reaction, to identify the presence of emerging parasites in faeces of patients susceptible to different infectious due to an immunocompromised status caused by diseases and chemotherapy to treat them. This method will help to decrease risk of complications and negative effects on life quality of ill patients.

Published in	American Journal of Bioscience and Bioengineering (Volume 2, Issue 2)
DOI	10.11648/j.bio.20140202.12
Page(s)	33-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Cryptosporidium, Microsporidium, Isospora, Belli, Cyclospora, Multiplex, Immunocompromised, Emerging, Parasites

References

[1]	Botero, D., Restrepo, M., Parasitosis humanas a ed. CIB, Colombia.
[2]	Flanigan, T., Whalen, C., Turner, J., .Cryptosporidium infection and CD count. Ann. Intern. Med.
[3]	Gonçalves, E.M., Araújo, R.S., Orban, M., Matté, G.R., Matté, M.H., Corbett, C.E. Protocol for DNA extraction of Cryptosporidium spp. oocysts in fecal samples. Rev. Inst. Med. Trop. Sao Paulo
[4]	Fayer, R., Morgan, U., Upton, S.J., Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol.
[5]	Bialek, R, Binder, N., Dietz, K., Knobloch, J., Zelck, U.E. Comparison of autofluorescence and iodine staining for detection of I. belli in feces. Am. J. Trop. Med. Hyg.
[6]	Mirdha, B.R., Kabra, S.K., Samantray, J.C., Isosporiasis in children. Indian. Pediatric.
[7]	Orlandi, P.A., Carter, L., Brinker, A.M., da Silva, A.J., Chu, D.M., Lampel, K.A., Monday, S.R., . Targeting single-nucleotide polymorphisms in the S rRNA gene to differentiate Cyclospora species from Eimeria species by multiplex PCR. Appl. Environ. Microbiol. , -.
[8]	Weitz, J.C., Weitz, C.R., Canales, M.R., Moya, R.R., Cyclospora cayetanensis infection: updated review a propos of three cases of traveler's diarrhea. Rev. Chilena. Infectol.
[9]	Wasson, K., Peper, R.L., Mammalian microsporidiosis. Vet. Pathol.
[10]	Viriyavejakul, P., Nintasen, R., Punsawad, C., Chaisri, U., Punpoowong, B., Riganti, M., . High prevalence of Microsporidium infection in HIV-infected patients. Southeast. Asian. J.Trop. Med. Public. Health. , -.
[11]	Di Gliullo, A.B., Cribari, M.S., Bava, A.J., Cicconetti, J.S., Collazos, R., Cyclospora cayetanensis in sputum and stool samples. Rev. Inst. Med. Trop. Sao Paulo. , -.
[12]	Geurden, T., Berkvens, D., Casaert, S., Vercruysse, J., Claerebout, E. A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogs. Vet. Parasitol.
[13]	Coyle, C.M., Wittner, M., Kotler, D.P., Noyer, C., Orenstein, J.M., Tanowitz, H.B., Weiss, L.M., Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by polymerase chain reaction to the microsporidian small-subunit rRNA gene. Clin. Infect. Dis.
[14]	Faust, E.C., D'Antoni, J.S., Odom, V., Miller, M.J., Peres, C., Sawitz, W., Thomen, L.F., Tobie, L.E., Walker, J.H., A critical study of clinical laboratory techniques for the diagnosis of protozoan cysts and helminth eggs in feces. Am. J. Trop. Med.
[15]	Visvesvara, G.S., Moura. H., Kovacs-Nace, E., Wallace, S., Eberhard, M.L., Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J. Clin. Microbiol.
[16]	Dong, J., Olano, J.P., McBride, J.W., Walker, D.H., Emerging pathogens: challenges and successes of molecular diagnostics. J. Mol. Diagn.

Cite This Article

Plain Text BibTeX RIS

APA Style

Enedina Jiménez-Cardoso, Apolinar Cano-Estrada, Adrian-Cortes Campos, Leticia Eligio-García. (2014). Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites. American Journal of Bioscience and Bioengineering, 2(2), 33-36. https://doi.org/10.11648/j.bio.20140202.12

Copy | Download

ACS Style

Enedina Jiménez-Cardoso; Apolinar Cano-Estrada; Adrian-Cortes Campos; Leticia Eligio-García. Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites. Am. J. BioSci. Bioeng. 2014, 2(2), 33-36. doi: 10.11648/j.bio.20140202.12

Copy | Download

AMA Style

Enedina Jiménez-Cardoso, Apolinar Cano-Estrada, Adrian-Cortes Campos, Leticia Eligio-García. Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites. Am J BioSci Bioeng. 2014;2(2):33-36. doi: 10.11648/j.bio.20140202.12

Copy | Download

@article{10.11648/j.bio.20140202.12,
  author = {Enedina Jiménez-Cardoso and Apolinar Cano-Estrada and Adrian-Cortes Campos and Leticia Eligio-García},
  title = {Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {2},
  number = {2},
  pages = {33-36},
  doi = {10.11648/j.bio.20140202.12},
  url = {https://doi.org/10.11648/j.bio.20140202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20140202.12},
  abstract = {The purpose of this study was to establish the optimal conditions to performance a multiple Polymerase Chain Reaction (multiplex) to detect the presence of emerging parasites Cryptosporidium spp., Mycrosporidium spp., Isospora belli, and Cyclospora spp. simultaneously, by targeting the 16S ribosomal gene in patients immunocompromised. After an exhaustive genetic analysis to obtain different primers sequences we tested the conditions of reagents concentration, time and temperature of reaction. The results showed the optimal conditions to make an efficient, specific, and reliable diagnostic method, performed in a simultaneous molecular reaction, to identify the presence of emerging parasites in faeces of patients susceptible to different infectious due to an immunocompromised status caused by diseases and chemotherapy to treat them. This method will help to decrease risk of complications and negative effects on life quality of ill patients.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites
AU  - Enedina Jiménez-Cardoso
AU  - Apolinar Cano-Estrada
AU  - Adrian-Cortes Campos
AU  - Leticia Eligio-García
Y1  - 2014/07/30
PY  - 2014
N1  - https://doi.org/10.11648/j.bio.20140202.12
DO  - 10.11648/j.bio.20140202.12
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 33
EP  - 36
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20140202.12
AB  - The purpose of this study was to establish the optimal conditions to performance a multiple Polymerase Chain Reaction (multiplex) to detect the presence of emerging parasites Cryptosporidium spp., Mycrosporidium spp., Isospora belli, and Cyclospora spp. simultaneously, by targeting the 16S ribosomal gene in patients immunocompromised. After an exhaustive genetic analysis to obtain different primers sequences we tested the conditions of reagents concentration, time and temperature of reaction. The results showed the optimal conditions to make an efficient, specific, and reliable diagnostic method, performed in a simultaneous molecular reaction, to identify the presence of emerging parasites in faeces of patients susceptible to different infectious due to an immunocompromised status caused by diseases and chemotherapy to treat them. This method will help to decrease risk of complications and negative effects on life quality of ill patients.
VL  - 2
IS  - 2
ER  -

Copy | Download

Author Information

Enedina Jiménez-Cardoso

Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
Apolinar Cano-Estrada

Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
Adrian-Cortes Campos

Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
Leticia Eligio-García

Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Enedina Jiménez-Cardoso, Apolinar Cano-Estrada, Adrian-Cortes Campos, Leticia Eligio-García. (2014). Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites. American Journal of Bioscience and Bioengineering, 2(2), 33-36. https://doi.org/10.11648/j.bio.20140202.12

Copy | Download

ACS Style

Enedina Jiménez-Cardoso; Apolinar Cano-Estrada; Adrian-Cortes Campos; Leticia Eligio-García. Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites. Am. J. BioSci. Bioeng. 2014, 2(2), 33-36. doi: 10.11648/j.bio.20140202.12

Copy | Download

AMA Style

Enedina Jiménez-Cardoso, Apolinar Cano-Estrada, Adrian-Cortes Campos, Leticia Eligio-García. Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites. Am J BioSci Bioeng. 2014;2(2):33-36. doi: 10.11648/j.bio.20140202.12

Copy | Download

@article{10.11648/j.bio.20140202.12,
  author = {Enedina Jiménez-Cardoso and Apolinar Cano-Estrada and Adrian-Cortes Campos and Leticia Eligio-García},
  title = {Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {2},
  number = {2},
  pages = {33-36},
  doi = {10.11648/j.bio.20140202.12},
  url = {https://doi.org/10.11648/j.bio.20140202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20140202.12},
  abstract = {The purpose of this study was to establish the optimal conditions to performance a multiple Polymerase Chain Reaction (multiplex) to detect the presence of emerging parasites Cryptosporidium spp., Mycrosporidium spp., Isospora belli, and Cyclospora spp. simultaneously, by targeting the 16S ribosomal gene in patients immunocompromised. After an exhaustive genetic analysis to obtain different primers sequences we tested the conditions of reagents concentration, time and temperature of reaction. The results showed the optimal conditions to make an efficient, specific, and reliable diagnostic method, performed in a simultaneous molecular reaction, to identify the presence of emerging parasites in faeces of patients susceptible to different infectious due to an immunocompromised status caused by diseases and chemotherapy to treat them. This method will help to decrease risk of complications and negative effects on life quality of ill patients.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites
AU  - Enedina Jiménez-Cardoso
AU  - Apolinar Cano-Estrada
AU  - Adrian-Cortes Campos
AU  - Leticia Eligio-García
Y1  - 2014/07/30
PY  - 2014
N1  - https://doi.org/10.11648/j.bio.20140202.12
DO  - 10.11648/j.bio.20140202.12
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 33
EP  - 36
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20140202.12
AB  - The purpose of this study was to establish the optimal conditions to performance a multiple Polymerase Chain Reaction (multiplex) to detect the presence of emerging parasites Cryptosporidium spp., Mycrosporidium spp., Isospora belli, and Cyclospora spp. simultaneously, by targeting the 16S ribosomal gene in patients immunocompromised. After an exhaustive genetic analysis to obtain different primers sequences we tested the conditions of reagents concentration, time and temperature of reaction. The results showed the optimal conditions to make an efficient, specific, and reliable diagnostic method, performed in a simultaneous molecular reaction, to identify the presence of emerging parasites in faeces of patients susceptible to different infectious due to an immunocompromised status caused by diseases and chemotherapy to treat them. This method will help to decrease risk of complications and negative effects on life quality of ill patients.
VL  - 2
IS  - 2
ER  -

Copy | Download