Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine

Aniekan Imo Peter; Gabriel Joseph Ekandem; Anozeng Oyono Igiri; Moses Bassey Ekong; Dianabasi King Udoh

doi:doi:10.11648/j.bio.20150303.11

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Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine

Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 3)

Received: 30 July 2015 Accepted: 10 August 2015 Published: 19 August 2015

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Abstract

Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution

Published in	American Journal of Bioscience and Bioengineering (Volume 3, Issue 3)
DOI	10.11648/j.bio.20150303.11
Page(s)	17-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Zidovudine, Cerebellum, Human Immunodeficiency Virus

References

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APA Style

Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. (2015). Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. American Journal of Bioscience and Bioengineering, 3(3), 17-21. https://doi.org/10.11648/j.bio.20150303.11

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ACS Style

Aniekan Imo Peter; Gabriel Joseph Ekandem; Anozeng Oyono Igiri; Moses Bassey Ekong; Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am. J. BioSci. Bioeng. 2015, 3(3), 17-21. doi: 10.11648/j.bio.20150303.11

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AMA Style

Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am J BioSci Bioeng. 2015;3(3):17-21. doi: 10.11648/j.bio.20150303.11

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@article{10.11648/j.bio.20150303.11,
  author = {Aniekan Imo Peter and Gabriel Joseph Ekandem and Anozeng Oyono Igiri and Moses Bassey Ekong and Dianabasi King Udoh},
  title = {Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {3},
  pages = {17-21},
  doi = {10.11648/j.bio.20150303.11},
  url = {https://doi.org/10.11648/j.bio.20150303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150303.11},
  abstract = {Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution},
 year = {2015}
}

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TY  - JOUR
T1  - Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine
AU  - Aniekan Imo Peter
AU  - Gabriel Joseph Ekandem
AU  - Anozeng Oyono Igiri
AU  - Moses Bassey Ekong
AU  - Dianabasi King Udoh
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150303.11
DO  - 10.11648/j.bio.20150303.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 17
EP  - 21
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150303.11
AB  - Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution
VL  - 3
IS  - 3
ER  -

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Author Information

Aniekan Imo Peter

Department of Anatomy, University of Uyo, Uyo, Nigeria
Gabriel Joseph Ekandem

Department of Anatomy, University of Uyo, Uyo, Nigeria
Anozeng Oyono Igiri

Department of Anatomy, University of Calabar, Uyo, Nigeriap
Moses Bassey Ekong

Department of Anatomy, University of Uyo, Uyo, Nigeria
Dianabasi King Udoh

Chemical Pathology Unit, Med Lab Services, University of Uyo Teaching Hospital, Uyo, Nigeria

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Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. (2015). Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. American Journal of Bioscience and Bioengineering, 3(3), 17-21. https://doi.org/10.11648/j.bio.20150303.11

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ACS Style

Aniekan Imo Peter; Gabriel Joseph Ekandem; Anozeng Oyono Igiri; Moses Bassey Ekong; Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am. J. BioSci. Bioeng. 2015, 3(3), 17-21. doi: 10.11648/j.bio.20150303.11

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AMA Style

Aniekan Imo Peter, Gabriel Joseph Ekandem, Anozeng Oyono Igiri, Moses Bassey Ekong, Dianabasi King Udoh. Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine. Am J BioSci Bioeng. 2015;3(3):17-21. doi: 10.11648/j.bio.20150303.11

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@article{10.11648/j.bio.20150303.11,
  author = {Aniekan Imo Peter and Gabriel Joseph Ekandem and Anozeng Oyono Igiri and Moses Bassey Ekong and Dianabasi King Udoh},
  title = {Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {3},
  pages = {17-21},
  doi = {10.11648/j.bio.20150303.11},
  url = {https://doi.org/10.11648/j.bio.20150303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150303.11},
  abstract = {Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution},
 year = {2015}
}

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TY  - JOUR
T1  - Histochemical Changes in the Cerebellum of Wistar Rats Administered with Oral Doses of Zidovudine
AU  - Aniekan Imo Peter
AU  - Gabriel Joseph Ekandem
AU  - Anozeng Oyono Igiri
AU  - Moses Bassey Ekong
AU  - Dianabasi King Udoh
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150303.11
DO  - 10.11648/j.bio.20150303.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 17
EP  - 21
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150303.11
AB  - Zidovudine is a drugs used in the management of Human Immunodeficiency Virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) infection in sub-Saharan Africa in combination with other drugs. The objective of this research was to investigate the potential harmful effects of this drug on the histology of the cerebellum of Wistar rats. Twenty male Wistar rats were used for this study. The rats were divided into 2 groups of 10 rats each. Group A served as the control and was administered with 1 ml of distill water, while group B was administered with 8.57mg/kg of zidovudine daily for 30 days, after which the rats where sacrificed and each cerebellum was harvested, processed and stained using haematoxylin and Eosin (H/E), silver impregnation method. Paraffin impregnated Glial Fibrilar Acidic Protein (GFAP), Neuron Specific Enolase (NSE) and Neurofilament (NF) immunochemistry methods. Stained slides were viewed using light microscope. Results showed that, the cerebellum of Groups B animals were affected with moderate to severe shrinking and distortion of the Purkinje cells and granular cells, when compared with the control. Group B animals, also showed more expression of GFAP, NSE and NF staining in their cerebellum than the control. This suggests that zidovudine is harmful to the cerebellum and should be taken with caution
VL  - 3
IS  - 3
ER  -

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