Cyclophosphamide (CP) is an alkylating agent widely used in cancer chemotherapy. The present study was undertaken to evaluate the natural protective efficacy of methanolic grape seed extract (GSE) against CP-induced genotoxicity and histopathologically changes of liver and colon cancer cells in mice. After the mice were sacrificed on day 30 liver and colons were removed, the histopathological studies and expression of colon cyclooxygenase-2(COX-2), was immunohistochemically assessed. High dose of GSE had a significant reduction of micronuclei and chromosomal aberration frequency than low dose, Mice treated with grape seeds (low & high) dose displayed an improvement in colonic histopathology compared to cyclophosphamide-only mice. Histopathological results showed the presence bridging necrosis with early fibrosis between portal tract and central veins with dysplastic hepatocytes in the liver tissue, also cellular infiltration, epithelial disruption, aberrant changes in the form neoplasia, dysplasia of the colonic mucosa. However, grape seed extract treatment to cyclophosphamide treated rats greatly restored normalcy in the liver and colonic histoarchitecture, with no apparent signs of neoplasia or displisia. Immunohistochemistry of COX-2 showed an intense staining in the liver colon tissues; however treatment with grape seed reduced the expression of COX-2. Treatment with grape seed showed time -dependently accelerated liver and colonic healing. In conclusion, the results of our experiments strongly suggest the cancer chemoprotective effect of the methanolic Grape seeds extract and this may be due to the stimulation of the antioxidant effect.
| Published in | Cell Biology (Volume 2, Issue 3) |
| DOI | 10.11648/j.cb.20140203.11 |
| Page(s) | 18-27 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Cyclophosphamide, Chromosome, Histopathology, Grape Seed Extract, Mice
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APA Style
Ezzat I. Aboul-ela, Enayat A. Omara. (2014). Genotoxic and Histopathological Aspects of Treatment with Grape Seed Extract on Cancer Induced with Cyclophosphamide in Mice. Cell Biology, 2(3), 18-27. https://doi.org/10.11648/j.cb.20140203.11
ACS Style
Ezzat I. Aboul-ela; Enayat A. Omara. Genotoxic and Histopathological Aspects of Treatment with Grape Seed Extract on Cancer Induced with Cyclophosphamide in Mice. Cell Biol. 2014, 2(3), 18-27. doi: 10.11648/j.cb.20140203.11
AMA Style
Ezzat I. Aboul-ela, Enayat A. Omara. Genotoxic and Histopathological Aspects of Treatment with Grape Seed Extract on Cancer Induced with Cyclophosphamide in Mice. Cell Biol. 2014;2(3):18-27. doi: 10.11648/j.cb.20140203.11
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Download@article{10.11648/j.cb.20140203.11,
author = {Ezzat I. Aboul-ela and Enayat A. Omara},
title = {Genotoxic and Histopathological Aspects of Treatment with Grape Seed Extract on Cancer Induced with Cyclophosphamide in Mice},
journal = {Cell Biology},
volume = {2},
number = {3},
pages = {18-27},
doi = {10.11648/j.cb.20140203.11},
url = {https://doi.org/10.11648/j.cb.20140203.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20140203.11},
abstract = {Cyclophosphamide (CP) is an alkylating agent widely used in cancer chemotherapy. The present study was undertaken to evaluate the natural protective efficacy of methanolic grape seed extract (GSE) against CP-induced genotoxicity and histopathologically changes of liver and colon cancer cells in mice. After the mice were sacrificed on day 30 liver and colons were removed, the histopathological studies and expression of colon cyclooxygenase-2(COX-2), was immunohistochemically assessed. High dose of GSE had a significant reduction of micronuclei and chromosomal aberration frequency than low dose, Mice treated with grape seeds (low & high) dose displayed an improvement in colonic histopathology compared to cyclophosphamide-only mice. Histopathological results showed the presence bridging necrosis with early fibrosis between portal tract and central veins with dysplastic hepatocytes in the liver tissue, also cellular infiltration, epithelial disruption, aberrant changes in the form neoplasia, dysplasia of the colonic mucosa. However, grape seed extract treatment to cyclophosphamide treated rats greatly restored normalcy in the liver and colonic histoarchitecture, with no apparent signs of neoplasia or displisia. Immunohistochemistry of COX-2 showed an intense staining in the liver colon tissues; however treatment with grape seed reduced the expression of COX-2. Treatment with grape seed showed time -dependently accelerated liver and colonic healing. In conclusion, the results of our experiments strongly suggest the cancer chemoprotective effect of the methanolic Grape seeds extract and this may be due to the stimulation of the antioxidant effect.},
year = {2014}
}
TY - JOUR T1 - Genotoxic and Histopathological Aspects of Treatment with Grape Seed Extract on Cancer Induced with Cyclophosphamide in Mice AU - Ezzat I. Aboul-ela AU - Enayat A. Omara Y1 - 2014/07/30 PY - 2014 N1 - https://doi.org/10.11648/j.cb.20140203.11 DO - 10.11648/j.cb.20140203.11 T2 - Cell Biology JF - Cell Biology JO - Cell Biology SP - 18 EP - 27 PB - Science Publishing Group SN - 2330-0183 UR - https://doi.org/10.11648/j.cb.20140203.11 AB - Cyclophosphamide (CP) is an alkylating agent widely used in cancer chemotherapy. The present study was undertaken to evaluate the natural protective efficacy of methanolic grape seed extract (GSE) against CP-induced genotoxicity and histopathologically changes of liver and colon cancer cells in mice. After the mice were sacrificed on day 30 liver and colons were removed, the histopathological studies and expression of colon cyclooxygenase-2(COX-2), was immunohistochemically assessed. High dose of GSE had a significant reduction of micronuclei and chromosomal aberration frequency than low dose, Mice treated with grape seeds (low & high) dose displayed an improvement in colonic histopathology compared to cyclophosphamide-only mice. Histopathological results showed the presence bridging necrosis with early fibrosis between portal tract and central veins with dysplastic hepatocytes in the liver tissue, also cellular infiltration, epithelial disruption, aberrant changes in the form neoplasia, dysplasia of the colonic mucosa. However, grape seed extract treatment to cyclophosphamide treated rats greatly restored normalcy in the liver and colonic histoarchitecture, with no apparent signs of neoplasia or displisia. Immunohistochemistry of COX-2 showed an intense staining in the liver colon tissues; however treatment with grape seed reduced the expression of COX-2. Treatment with grape seed showed time -dependently accelerated liver and colonic healing. In conclusion, the results of our experiments strongly suggest the cancer chemoprotective effect of the methanolic Grape seeds extract and this may be due to the stimulation of the antioxidant effect. VL - 2 IS - 3 ER -
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