Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice

Hector Serrano; Guillermo Mora-Ramiro; Sheila Peña-Corona; Pablo León-Ortíz; Arturo Salame-Mendez; Enrique Mendieta-Márques; José Luis Gómez-Olivares; María Dolores García-Suárez

doi:doi:10.11648/j.cb.20140204.12

Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice

Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, Enrique Mendieta-Márques, José Luis Gómez-Olivares, María Dolores García-Suárez

Published in Cell Biology (Volume 2, Issue 4)

Received: 8 September 2014 Accepted: 20 September 2014 Published: 30 September 2014

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Abstract

Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.

Published in	Cell Biology (Volume 2, Issue 4)
DOI	10.11648/j.cb.20140204.12
Page(s)	36-40
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Phytoestrogens, Coumestrol, Testis Alterations, Serum Progesterone, Mice

References

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APA Style

Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. (2014). Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biology, 2(4), 36-40. https://doi.org/10.11648/j.cb.20140204.12

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ACS Style

Hector Serrano; Guillermo Mora-Ramiro; Sheila Peña-Corona; Pablo León-Ortíz; Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014, 2(4), 36-40. doi: 10.11648/j.cb.20140204.12

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AMA Style

Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014;2(4):36-40. doi: 10.11648/j.cb.20140204.12

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@article{10.11648/j.cb.20140204.12,
  author = {Hector Serrano and Guillermo Mora-Ramiro and Sheila Peña-Corona and Pablo León-Ortíz and Arturo Salame-Mendez and Enrique Mendieta-Márques and José Luis Gómez-Olivares and María Dolores García-Suárez},
  title = {Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice},
  journal = {Cell Biology},
  volume = {2},
  number = {4},
  pages = {36-40},
  doi = {10.11648/j.cb.20140204.12},
  url = {https://doi.org/10.11648/j.cb.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20140204.12},
  abstract = {Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and  sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.},
 year = {2014}
}

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TY  - JOUR
T1  - Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice
AU  - Hector Serrano
AU  - Guillermo Mora-Ramiro
AU  - Sheila Peña-Corona
AU  - Pablo León-Ortíz
AU  - Arturo Salame-Mendez
AU  - Enrique Mendieta-Márques
AU  - José Luis Gómez-Olivares
AU  - María Dolores García-Suárez
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.cb.20140204.12
DO  - 10.11648/j.cb.20140204.12
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 36
EP  - 40
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20140204.12
AB  - Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and  sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.
VL  - 2
IS  - 4
ER  -

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Author Information

Hector Serrano

Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico
Guillermo Mora-Ramiro

Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico
Sheila Peña-Corona
Pablo León-Ortíz

Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico
Arturo Salame-Mendez

Depts. of Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico
Enrique Mendieta-Márques

Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico
José Luis Gómez-Olivares

Dept. of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico
María Dolores García-Suárez

Depts. of Biology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico

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APA Style

Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. (2014). Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biology, 2(4), 36-40. https://doi.org/10.11648/j.cb.20140204.12

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ACS Style

Hector Serrano; Guillermo Mora-Ramiro; Sheila Peña-Corona; Pablo León-Ortíz; Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014, 2(4), 36-40. doi: 10.11648/j.cb.20140204.12

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AMA Style

Hector Serrano, Guillermo Mora-Ramiro, Sheila Peña-Corona, Pablo León-Ortíz, Arturo Salame-Mendez, et al. Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice. Cell Biol. 2014;2(4):36-40. doi: 10.11648/j.cb.20140204.12

Copy | Download

@article{10.11648/j.cb.20140204.12,
  author = {Hector Serrano and Guillermo Mora-Ramiro and Sheila Peña-Corona and Pablo León-Ortíz and Arturo Salame-Mendez and Enrique Mendieta-Márques and José Luis Gómez-Olivares and María Dolores García-Suárez},
  title = {Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice},
  journal = {Cell Biology},
  volume = {2},
  number = {4},
  pages = {36-40},
  doi = {10.11648/j.cb.20140204.12},
  url = {https://doi.org/10.11648/j.cb.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20140204.12},
  abstract = {Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and  sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Acute Oral Coumestrol Treatment Induces Sperm and Sex Steroid Alterations in Mice
AU  - Hector Serrano
AU  - Guillermo Mora-Ramiro
AU  - Sheila Peña-Corona
AU  - Pablo León-Ortíz
AU  - Arturo Salame-Mendez
AU  - Enrique Mendieta-Márques
AU  - José Luis Gómez-Olivares
AU  - María Dolores García-Suárez
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.cb.20140204.12
DO  - 10.11648/j.cb.20140204.12
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 36
EP  - 40
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20140204.12
AB  - Plant phytoestrogens interfere with normal estrogen-regulated functions like steroid synthesis and gonad physiology and morphology. Much evidence has been obtained by using high dose treatments or in vitro exposure to phytoestrogens but little is known about low, dietary level concentrations of these compounds, particularly coumestrol. In order to explore the possible effects on gonads and serum progesterone of oral 10, 20 or 40 μg/Kg body weight dose coumestrol were administered to three experimental groups and compared to a vehicle-only control group (n=5 animals per group) for two weeks and a similar period for treatment recovery. After treatment, testes and blood were obtained and processed for testis and  sperm morphology alterations, and steroid hormone evaluation, respectively. Coumestrol treatment induces a significant dose-dependent testis volume decrease and a decrease in 17β hydroxysteroid dehydrogenase activity causing a progesterone increase in response to phytoestrogen concentration. These alterations impair the normal sperm production with an increase in abnormal head and tail shapes. These data strongly suggest a deleterious effect of oral, low concentration phytoestrogen content in adult male diets.
VL  - 2
IS  - 4
ER  -

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