The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge

Cao Lu; Xue Fei; Liu Eryan; Li Dengxing; Asif Hussain; Taimore Hussain

doi:doi:10.11648/j.ijepp.20180602.14

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The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge

Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, Taimore Hussain

Published in International Journal of Environmental Protection and Policy (Volume 6, Issue 2)

Received: 20 June 2018 Published: 21 June 2018

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Abstract

The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.

Published in	International Journal of Environmental Protection and Policy (Volume 6, Issue 2)
DOI	10.11648/j.ijepp.20180602.14
Page(s)	42-49
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Alkaline Protease, Sodium Hexametaphosphate, Methyl Orange, Three-Dimensional Fluorescence Spectroscopy

References

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APA Style

Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, et al. (2018). The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. International Journal of Environmental Protection and Policy, 6(2), 42-49. https://doi.org/10.11648/j.ijepp.20180602.14

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ACS Style

Cao Lu; Xue Fei; Liu Eryan; Li Dengxing; Asif Hussain, et al. The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. Int. J. Environ. Prot. Policy 2018, 6(2), 42-49. doi: 10.11648/j.ijepp.20180602.14

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AMA Style

Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, et al. The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. Int J Environ Prot Policy. 2018;6(2):42-49. doi: 10.11648/j.ijepp.20180602.14

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@article{10.11648/j.ijepp.20180602.14,
  author = {Cao Lu and Xue Fei and Liu Eryan and Li Dengxing and Asif Hussain and Taimore Hussain},
  title = {The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge},
  journal = {International Journal of Environmental Protection and Policy},
  volume = {6},
  number = {2},
  pages = {42-49},
  doi = {10.11648/j.ijepp.20180602.14},
  url = {https://doi.org/10.11648/j.ijepp.20180602.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijepp.20180602.14},
  abstract = {The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.},
 year = {2018}
}

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TY - JOUR
T1 - The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge
AU - Cao Lu
AU - Xue Fei
AU - Liu Eryan
AU - Li Dengxing
AU - Asif Hussain
AU - Taimore Hussain
Y1 - 2018/06/21
PY - 2018
N1 - https://doi.org/10.11648/j.ijepp.20180602.14
DO - 10.11648/j.ijepp.20180602.14
T2 - International Journal of Environmental Protection and Policy
JF - International Journal of Environmental Protection and Policy
JO - International Journal of Environmental Protection and Policy
SP - 42
EP - 49
PB - Science Publishing Group
SN - 2330-7536
UR - https://doi.org/10.11648/j.ijepp.20180602.14
AB - The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.
VL - 6
IS - 2
ER -

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Author Information

Cao Lu

College of Environmental Science and Engineering, Donghua University, Shanghai, China
Xue Fei

College of Environmental Science and Engineering, Donghua University, Shanghai, China
Liu Eryan

College of Environmental Science and Engineering, Donghua University, Shanghai, China
Li Dengxing

College of Environmental Science and Engineering, Donghua University, Shanghai, China
Asif Hussain

College of Environmental Science and Engineering, Donghua University, Shanghai, China
Taimore Hussain

College of Environmental Science and Engineering, Donghua University, Shanghai, China

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APA Style

Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, et al. (2018). The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. International Journal of Environmental Protection and Policy, 6(2), 42-49. https://doi.org/10.11648/j.ijepp.20180602.14

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ACS Style

Cao Lu; Xue Fei; Liu Eryan; Li Dengxing; Asif Hussain, et al. The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. Int. J. Environ. Prot. Policy 2018, 6(2), 42-49. doi: 10.11648/j.ijepp.20180602.14

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AMA Style

Cao Lu, Xue Fei, Liu Eryan, Li Dengxing, Asif Hussain, et al. The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge. Int J Environ Prot Policy. 2018;6(2):42-49. doi: 10.11648/j.ijepp.20180602.14

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@article{10.11648/j.ijepp.20180602.14,
  author = {Cao Lu and Xue Fei and Liu Eryan and Li Dengxing and Asif Hussain and Taimore Hussain},
  title = {The Influences of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on Enzyme Hydrolysis of Excess Sludge},
  journal = {International Journal of Environmental Protection and Policy},
  volume = {6},
  number = {2},
  pages = {42-49},
  doi = {10.11648/j.ijepp.20180602.14},
  url = {https://doi.org/10.11648/j.ijepp.20180602.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijepp.20180602.14},
  abstract = {The effect of Sodium Hexametaphosphate (SHMP) and Methyl Orange (MO) on the hydrolysis of sludge were investigated under the optimum condition of alkaline protease hydrolysis. The results showed that as the concentration of SHMP was increased, the content of Soluble Chemical Oxygen Demand (SCOD), protein, and polysaccharide in the sludge hydrolysate gradually raised. When the concentration of SHMP was 0.1g/g·tss, the contents of SCOD, protein, and polysaccharide increased by 17.16%, 50%, and 9.72% respectively. With the increase of MO concentration, SCOD decreased gradually. When the concentration of SHMP was 0.02g/g·tss, the SCOD decreased by 10.3%. An increase of 13% in the protein content was noted, when the concentration was 0.02g/g·tss. On the contrary, there was a slight reduction of the polysaccharide content i.e. 2.4%. The concentration of the polysaccharide was found to be the lowest at the concentration of 0.002g/g·tss. From the aforementioned results combined with three-dimensional fluorescence map of sludge EPS, it was found that SHMP promoted the hydrolysis of sludge by alkaline protease and accelerated the dissolution of intracellular organic matter. Herein, the MO inhibited alkaline protease hydrolysis of sludge, which hindered the dissolution of intracellular organics.},
 year = {2018}
}

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