Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

Gerrard Eddy Jai Poinern; Xuan Le; Mark O’Dea; Derek Fawcett

doi:doi:10.11648/j.ijmsa.20140306.19

| Peer-Reviewed

Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate

Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett

Published in International Journal of Materials Science and Applications (Volume 3, Issue 6)

Received: 28 August 2014 Accepted: 11 September 2014 Published: 30 October 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.

Published in	International Journal of Materials Science and Applications (Volume 3, Issue 6)
DOI	10.11648/j.ijmsa.20140306.19
Page(s)	331-338
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Biomaterials, Anodic Aluminium Oxide, Bioceramics, Regenerative Medicine

References

[1]	G.E.J. Poinern, R. Shackleton, S.I. Mamun and D. Fawcett, Significance of novel bioinorganic anodic aluminium oxide nanoscaffolds for promoting cellular response. Nanotechnology, Science and Applications, vol. 3, pp. 1-14, 2010.
[2]	A.P. Li, F. Muller, A. Birner, K. Nielsch, U. Gosele, Hexagonal pore arrays with a 50–420 inter-pore distance formed by self-organization in anodic alumina. J. Appl. Phys., vol. 84, pp. 6023-6026, 1998.
[3]	J.P. O’Sullivan, G.C. Wood, Nucleation and growth of porous anodic films on aluminium. P. Roy. Lond. A. Mat, vol. A317, pp. 511-543, 1970.
[4]	H. Masuda, K. Yada, A. Osaka, Self-ordering of cell configuration of anodic porous alumina with large-size pores in phosphoric acid solution. Jpn. J. Appl. Phys. Vol. 37: pp. L1340-L1342, 1998.
[5]	M. Ghorbani, F. Nasirpouri, A. Iraji-zad, A. Saedi, On the growth sequence of highly ordered nano-porous anodic aluminium oxide. Mater. Des. Vol. 27: pp. 983-988, 2006.
[6]	D.H. Choi, P.S. Lee, W. Hwang, K.H. Lee, H.C. Park, Measurement of pore sizes for anodic aluminium oxide (AAO). Curr. Appl. Phys, vol. 6S1:pp. e125-e129, 2006.
[7]	G.E.J. Poinern, N. Ali, D. Fawcett, Progress in nano-engineered anodic aluminium oxide membrane development. Materials, vol. 4, pp. 487-526, 2011.
[8]	H. Masuda, M. Satoh, Fabrication of gold nano-dot array using anodic porous alumina as an evaporation mask. Jpn. J. Appl. Phys, vol. 35: pp. L126-L129, 1996.
[9]	S. Shingubara, Fabrication of nanomaterials using porous alumina templates. J. Nanoparticle Res, vol. 5, pp. 17-30, 2003.
[10]	X.J. Wu, F. Zhu, C. Mu, Y. Liang, L. Xu, Q. Chen, R. Chen, D. Xu, Electrochemical synthesis and applications of oriented and hierarchically quasi-1D semiconducting nanos tructures. Coord. Chem. Rev. Vol. 254: pp. 1135-1150, 2010.
[11]	Y. Kim, B. Jung, H. Lee, H. Kim, K. Lee, H. Park, Capacitive humidity sensor design based on aluminium oxide. Sensor. Actuator B, vol. 141, pp. 441-446, 2009.
[12]	R. Bogue, The fabrication and assembly of nano-electronic devices. Assembly Automation, vol. 30, pp. 206-212, 2010.
[13]	Martin CR, Membrane based synthesis of nanomaterials, Chem. Mater. Vol. 8, pp. 1739-1746, 1996.
[14]	G.E.J. Poinern, D. Fawcett, Y.J. Ng, N. Ali, R.K. Brundavanam, Z.T. Jiang, Nanoengineering a biocompatible inorganic scaffold for skin wound healing. J. BioMed. Nanotech, vol. 6, pp. 497-510, 2010.
[15]	D. Bruggemann, Nanoporous aluminium oxide membranes as cell substrates. Journal of Nanomaterials. Article ID 460870, pp. 1-18, 2013.
[16]	G.E.J. Poinern, N. Ali, C. Berry, P. Singh, and D. Fawcett, Biocompatibility of synthesis nano-porous anodic aluminium oxide membranes for use as a cell culture substrate for Madin-Darby Canine Kidney Cells: A preliminary study. J. Tissue Sci Eng., vol. 3, No. 119, pp. 1-7, 2012.
[17]	L.G. Parkinson, N.L. Giles, K.F. Adcroft, M.W. Fear, F.M. Wood, G.E.J. Poinern, The potential of nanoporous anodic aluminium oxide membranes to influence skin wound repair. Tissue Eng Part A. Vol. 15, No. 12, pp. 3753–3763, 2009.
[18]	A. Hoess, N. Teuscher, A. Thormann, H. Aurich, A. Heilmann, Cultivation of hepatoma cell line HepG2 on nanoporous aluminum oxide membranes. Acta Biomater. Vol. 3, No. 1, pp. 43–50, 2007.
[19]	D.C. Miller, A. Thapa, K.M. Haberstroh, T.J. Webster, Endothelial and vascular smooth muscle cell function on poly (lactic-co-glycolic acid) with nano-structure features. Biomaterials, vol. 25, No. 1, pp. 53–61, 2004.
[20]	M. Karlsson, E. Pålsgård, P.R. Wilshaw, L. di Silvio, Initial in vitro interaction of osteoblasts with nano-porous alumina. Biomaterials, vol. 24, No. 18, pp. 3039–3046, 2003.
[21]	P. Roach, D. Eglin, K. Rohde, C. Perry, Modern biomaterials: A review–Bulk properties and implications of surface modifications. J. Mater. Sci. Mater. Med, vol. 18, pp. 1263-1277, 2007.
[22]	E. Damien, P.A. Revell, Coralline hydroxyapatite bone substitute: A review of experimental studies and biomedical applications, J. Appl. Biomater. Biomech, vol. 2, pp. 65-73, 2004.
[23]	D.W. Hutmacher, J.T. Schantz, C.X.F. Lam, K.C. Tan, T.C. Lim, State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective, J. Tissue Eng. Regen. Med, vol. 1, pp. 245-260, 2007.
[24]	W.J.E.M. Habraken, J.G.C. Wolke, J.A. Jansen, Ceramic composites as matrices and scaffolds for drug delivery in tissue engineering, Adv. Drug Deli. Rev., vol. 59, pp. 234-248, 2007.
[25]	A. Blom A, Which scaffold for which application, Curr. Orthop, vol. 21, pp. 280-287, 2007.
[26]	P. Habibovic, K. de Groot, Osteoinductive biomaterials properties and relevance in bone repair, J. Tissue Eng and Regen. Med, vol. 1, pp. 25-32, 2007.
[27]	S.J. Kalita, A. Bhardwaj, H.A. Bhatt, Nanocrystalline calcium phosphate ceramics in biomedical engineering, Mater. Sci. Eng. C, vol. 27, pp. 441-449, 2007.
[28]	Virology Laboratory procedure VIW-17, Animal Health Laboratories, Animal Virology, Department of Agriculture and Food, 3 Baron Hay Court, Kensington, Western Australia 6151, Australia.
[29]	G.E.J. Poinern, X. Le, M. O’Dea, T. Becker, D. Fawcett, Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line: A Preliminary Study. BioMed Research International. Article ID 238762, pp. 1-10, 2014.
[30]	G.E.J. Poinern, X. Le, M. Hager, T. Becker, D. Fawcett, Electrochemical Synthesis, Characterisation, and Preliminary Biological Evaluation of an Anodic Aluminium Oxide Membrane with a pore size of 100 nanometres for a Potential Cell Culture Substrate. American Journal of Biomedical Engineering, vol. 3, No. 6, pp. 119-131, 2013.
[31]	C. Schafer, B. Borm, S. Born, C. Mohr, E.M. Eibl, B. Hoffman B, One step ahead: Role of filopodia in adhesion formation during cell migration of Keratinocytes. Experimental Cell Research, vol. 315, pp.1212-1224, 2009.

Cite This Article

Plain Text BibTeX RIS

APA Style

Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett. (2014). Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. International Journal of Materials Science and Applications, 3(6), 331-338. https://doi.org/10.11648/j.ijmsa.20140306.19

Copy | Download

ACS Style

Gerrard Eddy Jai Poinern; Xuan Le; Mark O’Dea; Derek Fawcett. Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. Int. J. Mater. Sci. Appl. 2014, 3(6), 331-338. doi: 10.11648/j.ijmsa.20140306.19

Copy | Download

AMA Style

Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett. Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. Int J Mater Sci Appl. 2014;3(6):331-338. doi: 10.11648/j.ijmsa.20140306.19

Copy | Download

@article{10.11648/j.ijmsa.20140306.19,
  author = {Gerrard Eddy Jai Poinern and Xuan Le and Mark O’Dea and Derek Fawcett},
  title = {Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate},
  journal = {International Journal of Materials Science and Applications},
  volume = {3},
  number = {6},
  pages = {331-338},
  doi = {10.11648/j.ijmsa.20140306.19},
  url = {https://doi.org/10.11648/j.ijmsa.20140306.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmsa.20140306.19},
  abstract = {In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate
AU  - Gerrard Eddy Jai Poinern
AU  - Xuan Le
AU  - Mark O’Dea
AU  - Derek Fawcett
Y1  - 2014/10/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijmsa.20140306.19
DO  - 10.11648/j.ijmsa.20140306.19
T2  - International Journal of Materials Science and Applications
JF  - International Journal of Materials Science and Applications
JO  - International Journal of Materials Science and Applications
SP  - 331
EP  - 338
PB  - Science Publishing Group
SN  - 2327-2643
UR  - https://doi.org/10.11648/j.ijmsa.20140306.19
AB  - In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.
VL  - 3
IS  - 6
ER  -

Copy | Download

Author Information

Gerrard Eddy Jai Poinern

Department of Physics, Energy Studies and Nanotechnology School of Engineering and Energy, Murdoch University, Perth, Western Australia, Australia
Xuan Le

Department of Physics, Energy Studies and Nanotechnology School of Engineering and Energy, Murdoch University, Perth, Western Australia, Australia
Mark O’Dea

Department of Agriculture and Food, State Government of Western Australia, Kensington, Western Australia, Australia
Derek Fawcett

Department of Physics, Energy Studies and Nanotechnology School of Engineering and Energy, Murdoch University, Perth, Western Australia, Australia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett. (2014). Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. International Journal of Materials Science and Applications, 3(6), 331-338. https://doi.org/10.11648/j.ijmsa.20140306.19

Copy | Download

ACS Style

Gerrard Eddy Jai Poinern; Xuan Le; Mark O’Dea; Derek Fawcett. Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. Int. J. Mater. Sci. Appl. 2014, 3(6), 331-338. doi: 10.11648/j.ijmsa.20140306.19

Copy | Download

AMA Style

Gerrard Eddy Jai Poinern, Xuan Le, Mark O’Dea, Derek Fawcett. Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate. Int J Mater Sci Appl. 2014;3(6):331-338. doi: 10.11648/j.ijmsa.20140306.19

Copy | Download

@article{10.11648/j.ijmsa.20140306.19,
  author = {Gerrard Eddy Jai Poinern and Xuan Le and Mark O’Dea and Derek Fawcett},
  title = {Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate},
  journal = {International Journal of Materials Science and Applications},
  volume = {3},
  number = {6},
  pages = {331-338},
  doi = {10.11648/j.ijmsa.20140306.19},
  url = {https://doi.org/10.11648/j.ijmsa.20140306.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmsa.20140306.19},
  abstract = {In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Can Anodic Aluminium Oxide Nanomembranes Treated with Nanometre Scale Hydroxyapatite be Used as a Cell Culture Substrate
AU  - Gerrard Eddy Jai Poinern
AU  - Xuan Le
AU  - Mark O’Dea
AU  - Derek Fawcett
Y1  - 2014/10/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijmsa.20140306.19
DO  - 10.11648/j.ijmsa.20140306.19
T2  - International Journal of Materials Science and Applications
JF  - International Journal of Materials Science and Applications
JO  - International Journal of Materials Science and Applications
SP  - 331
EP  - 338
PB  - Science Publishing Group
SN  - 2327-2643
UR  - https://doi.org/10.11648/j.ijmsa.20140306.19
AB  - In this study we investigate the biomedical potential of composite membranes composed of anodic aluminium oxide (AAO) and nanometre scale hydroxyapatite (HAP). The nano-porous AAO membranes were produced using a temperature controlled two-step anodization technique. The AAO/HAP composite membranes were formed using the solution template wetting technique. The Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line was used to demonstrate the biocompatibility of the synthesised membranes and composites. Investigating cell adhesion, morphology and proliferation over a 72 h period assessed cellular interactions and responses of the cell line to the various membranes types.
VL  - 3
IS  - 6
ER  -

Copy | Download