The aim of this study was to investigate the causes of E. coli contamination of Cheddar cheese through the application of Hazard Analysis Critical Control Points (HACCP) principles. Cheese samples were analyzed for E. coli and coliforms after production, during the validation stage, as well as at the verification stage. Average E. coli and coliform counts were analyzed statistically using the t-test. Results showed that after the implementation of the corrective measures there was a decrease in E. coli and coliform counts at the 5% level of significance. Results presented in this study also show that manufacturing Cheddar cheese whole observing high standards of hygiene improves the reduces E. coli and coliform contamination of the product, even though the problem is not completely eliminated.
Published in |
International Journal of Nutrition and Food Sciences (Volume 3, Issue 6-1)
This article belongs to the Special Issue Optimizing Quality and Food Process Assessment |
DOI | 10.11648/j.ijnfs.s.2014030601.12 |
Page(s) | 6-14 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Validation, Hazard Analysis Critical Points (HACCP), Pathogenic Microorganisms, Coliform Count
[1] | Ahmed A.H, Moustafa M, T.A, 1986, Growth and survival of Yersinia enterocolitica in yoghurt, J. Fd Prot 49 Anonymous, 2003, Analysis of microbial hazards related to time/temperature |
[2] | Babel, F. J. 1977, Antibiosis by lactic culture bacteria. J. Dairy Sci. 60:815–821. |
[3] | Buazzi, M. M., M. E. Johnson, and E. H. Marth. 1992, Survival of L. monocytogenes during the manufacture and ripening of Swiss cheese. J. Dairy Sci. 75(2):380–386. |
[4] | Buchanan, R. L., E. L. Harden, and R. D. Beaulieu. 1999, Date marking of cheese. FDA Program Information Manual-Retail Food Safety. |
[5] | Duh, Y-H, and D. W. Schaffner. 1993, Modelling the effect of temperature on the growth rate and lag time of L. innocua and L. monocytogenes. J. Food Prot. 56(3):205–210. |
[6] | Frye, C., and C. W. Donnelly. 2005. Comprehensive survey of pasteurized fluid milk produced in the United States reveals a low prevalence of L. monocytogenes. J. FoodProt. 68(5):973–979. |
[7] | Fox P.F, Paul L.H, McSweeney T, Timothy M C, Timothy G, 2000. Cheese: Chemistry, Physics and Microbiology Aspects, Vol 1, Academic Press. |
[8] | Gengeorgis, C., M. Carniciu, D.Dutulescu, and T. B. Farver. 1991. Growth and survival of L. monocytogenes in market cheeses stored at 4 to 30 ° C. J. Food Prot. 54 |
[9] | Goepfert, J. M., N. F. Olson, and E. H. Marth. 1968, Behaviour of Salmonella Typhimurium during manufacture and curing of Cheddar cheese. Appl. Microbial. 16(6):862–866. |
[10] | Hargrove, R. E., F. E. McDonough, and W. A. Mattingly. 1969. Factors affecting survival of Salmonella in Cheddar and Colby cheese. J. Milk Food Technol. 32:480–484. |
[11] | International Dairy Federation.1980. Behaviour of pathogens in cheese. IDF Bulletin 122. |
[12] | Johnson, E. A., J. H. Nelson, and M. E. Johnson. 1990. Microbiological safety of cheese made from heat-treated milk, Part I. Executive summary, introduction and history. J. Food Prot. 53(5):441–452. |
[13] | Johnson, E. A., J. H. Nelson, and M. E. Johnson. 1990. Microbiological safety of cheese made from heat-treated milk, Part II. Microbiology. J. Food Prot. 53(6):519–540.40. |
[14] | Johnson, E. A., J. H. Nelson, and M. E. Johnson. 1990. Microbiological safety of cheese made from heat-treated milk, Part III. Technology, discussion, recommendations, bibliography. J. Food Prot. 53(7):610–623. |
[15] | Kornacki, J. L., and E. H. Marth.1982. Fate of non-pathogenic and enteropathogenic Escherichia coli during the manufacture of Colby-like cheese. J. Food Prot. 45(4):310–316. |
[16] | Kosikowski, F. V., and V. V. Mistry.1997. Cheese and Fermented Milk Products. 3rd ed., vol. 1, p. 328–352.FV Kosikowski, Westport, CT. |
[17] | Leistner, X. 1978. Hurdle effect and energy saving. W. K. Downey (ed.) Food Quality and Nutrition. Applied Science Publishers. London. |
[18] | Leedom. J, M, 2006, Milk of nonhuman origin and infectious diseases in humans, Clin. Infect. Dis 43 (5). |
[19] | Mathew, F. P., and E. T. Ryser. 2002. Competition of thermally injured L. monocytogenes with a Mesophilic lactic acid starter culture in milk for various heat treatments. J. Food Prot. 65(4):643–650. |
[20] | McDowall, F.H, 1987, the butter maker’s manual, Wellington. |
[21] | Moustafa, M. K., A. A. -H Ahmed, and E. H. Marth. 1983. Behaviour of virulent Yersinia enterocolitica during manufacture and storage of Colby-like cheese. J. Food Prot. 46(4):318 |
[22] | Norholt, M. D., 1984. Growth and inactivation of pathogenic microorganisms during manufacture and storage of fermented dairy products: A review. Neth. Milk Dairy J.38 |
[23] | Oliver, S.P, Boor, K.J, Murphy, S.C, Murinda.S,E, 2009,Food Safety hazards associated with consumption of raw milk, Food borne Pathog Dis 6 (7) |
[24] | Park, H. S., E. H. Marth, J. M. Goepfert, and N. F. Olson. 1970. The fate of Salmonella Typhimurium in the manufacture and ripening of low-acid Cheddar cheese. J. Milk FoodTechnol. 33:280–284. |
[25] | Rice, E.B, 1981, Queensland agric, J, 50, 708. |
[26] | Reiter, B. 1985. Interaction between immunoglobulins and innate factors such as lysozyme, lactoferrin, lactoperoxidase. J. Schaub (ed.) compos. Physiol. Prop. Human Milk, Proc. Intern. Workshop, Elsevier, Amsterdam pp. 271–284. |
[27] | Ryser, E. T., and E. H. Marth. 1991, .Incidence and behaviour of L. monocytogenes in cheese and other fermented dairy products. L. monocytogenes, Listeriosis and Food Safety. L. monocytogenes in Fermented Dairy Products, pp. 331–404. |
[28] | R. Early, 1998. Liquid Milk and Cream. In The Technology of Dairy Products, Ed. R. Early, Blackie Academic and Professional, 1998. |
[29] | Schaak, M. M., and E. H. Marth. 1988, Interaction between lactic acid bacteria and some foodborne pathogens: A review. Cultured Prod. J.Nov: 14–20 |
[30] | Spahr, U., and B. Url. 1994, Behaviour of pathogenic bacteria in cheese –A synopsis of experimental data.IDF bulletin 298:2–16. |
[31] | Sutherland, J. P., A. J. Bayliss, and T. A. Roberts. 1994. Predictive modelling of growth of Staphylococcus aureus: the effects of temperature, pH and sodium chloride. Intern.J. Food Microbiol. 21:217–236 |
APA Style
Amanda Kwenda, Moses Nyahada, Amos Musengi, Misheck Mudyiwa, Perkins Muredzi. (2014). An Investigation on the Causes of Escherichia coli and Coliform Contamination of Cheddar Cheese and How to Reduce the Problem (A Case Study at a Cheese Manufacturing Firm in Harare, Zimbabwe). International Journal of Nutrition and Food Sciences, 3(6-1), 6-14. https://doi.org/10.11648/j.ijnfs.s.2014030601.12
ACS Style
Amanda Kwenda; Moses Nyahada; Amos Musengi; Misheck Mudyiwa; Perkins Muredzi. An Investigation on the Causes of Escherichia coli and Coliform Contamination of Cheddar Cheese and How to Reduce the Problem (A Case Study at a Cheese Manufacturing Firm in Harare, Zimbabwe). Int. J. Nutr. Food Sci. 2014, 3(6-1), 6-14. doi: 10.11648/j.ijnfs.s.2014030601.12
AMA Style
Amanda Kwenda, Moses Nyahada, Amos Musengi, Misheck Mudyiwa, Perkins Muredzi. An Investigation on the Causes of Escherichia coli and Coliform Contamination of Cheddar Cheese and How to Reduce the Problem (A Case Study at a Cheese Manufacturing Firm in Harare, Zimbabwe). Int J Nutr Food Sci. 2014;3(6-1):6-14. doi: 10.11648/j.ijnfs.s.2014030601.12
@article{10.11648/j.ijnfs.s.2014030601.12, author = {Amanda Kwenda and Moses Nyahada and Amos Musengi and Misheck Mudyiwa and Perkins Muredzi}, title = {An Investigation on the Causes of Escherichia coli and Coliform Contamination of Cheddar Cheese and How to Reduce the Problem (A Case Study at a Cheese Manufacturing Firm in Harare, Zimbabwe)}, journal = {International Journal of Nutrition and Food Sciences}, volume = {3}, number = {6-1}, pages = {6-14}, doi = {10.11648/j.ijnfs.s.2014030601.12}, url = {https://doi.org/10.11648/j.ijnfs.s.2014030601.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.s.2014030601.12}, abstract = {The aim of this study was to investigate the causes of E. coli contamination of Cheddar cheese through the application of Hazard Analysis Critical Control Points (HACCP) principles. Cheese samples were analyzed for E. coli and coliforms after production, during the validation stage, as well as at the verification stage. Average E. coli and coliform counts were analyzed statistically using the t-test. Results showed that after the implementation of the corrective measures there was a decrease in E. coli and coliform counts at the 5% level of significance. Results presented in this study also show that manufacturing Cheddar cheese whole observing high standards of hygiene improves the reduces E. coli and coliform contamination of the product, even though the problem is not completely eliminated.}, year = {2014} }
TY - JOUR T1 - An Investigation on the Causes of Escherichia coli and Coliform Contamination of Cheddar Cheese and How to Reduce the Problem (A Case Study at a Cheese Manufacturing Firm in Harare, Zimbabwe) AU - Amanda Kwenda AU - Moses Nyahada AU - Amos Musengi AU - Misheck Mudyiwa AU - Perkins Muredzi Y1 - 2014/09/17 PY - 2014 N1 - https://doi.org/10.11648/j.ijnfs.s.2014030601.12 DO - 10.11648/j.ijnfs.s.2014030601.12 T2 - International Journal of Nutrition and Food Sciences JF - International Journal of Nutrition and Food Sciences JO - International Journal of Nutrition and Food Sciences SP - 6 EP - 14 PB - Science Publishing Group SN - 2327-2716 UR - https://doi.org/10.11648/j.ijnfs.s.2014030601.12 AB - The aim of this study was to investigate the causes of E. coli contamination of Cheddar cheese through the application of Hazard Analysis Critical Control Points (HACCP) principles. Cheese samples were analyzed for E. coli and coliforms after production, during the validation stage, as well as at the verification stage. Average E. coli and coliform counts were analyzed statistically using the t-test. Results showed that after the implementation of the corrective measures there was a decrease in E. coli and coliform counts at the 5% level of significance. Results presented in this study also show that manufacturing Cheddar cheese whole observing high standards of hygiene improves the reduces E. coli and coliform contamination of the product, even though the problem is not completely eliminated. VL - 3 IS - 6-1 ER -