A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production

Ramaraj Rameshprabu; Rungthip Kawaree; Yuwalee Unpaprom

doi:doi:10.11648/j.ijrse.s.2015040101.12

| Peer-Reviewed

A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production

Ramaraj Rameshprabu, Rungthip Kawaree, Yuwalee Unpaprom

Published in International Journal of Sustainable and Green Energy (Volume 4, Issue 1-1)

Received: 2 November 2014 Accepted: 7 November 2014 Published: 11 January 2015

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Biofuels are gaining attention worldwide as a way to reduce the dependence on fossil fuels. Biological Hydrogen (H2) production is considered the most environmentally friendly route of producing H2, fulﬁlling the goals of recycling renewable resources and producing clean energy. It has attracted global attention because of its potential to become an inexhaustible, low cost, renewable source of clean energy and appears as an alternative fuel. H2 production processes offer a technique through which renewable energy sources like biomass can be utilized for the generation of the cleanest energy carrier for the use of mankind. This paper presents laboratory results of biological production of hydrogen by green alga was isolated from fresh water fish pond in Sansai, Chiang Mai province, Thailand. Under light microscope, this green alga was identified as belonging to the genus Pediastrum and species P. duplex Meyen. The successful culture was established and grown in poultry litter effluent medium (PLEM) under a light intensity of 37.5 μmol-1m2 sec-1 and a temperature of 25°C. The nutrient requirements and process conditions that encourage the growth of dense and healthy algal cultures were explored. The highest H2 was produced when cultivated cells in PLEM for 21 hours under light and then incubated under anaerobic adaptation for 4 hours.

Published in	International Journal of Sustainable and Green Energy (Volume 4, Issue 1-1) This article belongs to the Special Issue Renewable Energy Applications in the Agricultural Field and Natural Resource Technology
DOI	10.11648/j.ijrse.s.2015040101.12
Page(s)	7-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Freshwater Algae, Pediastrum Duplex Meyen, Poultry Litter Effluent, Biohydrogen

References

[1]	R. Ramaraj, D. D-W. Tsai, P. H. Chen, “Algae Growth in Natural Water Resources”, Journal of Soil and Water Conservation, 2010, 42: 439–450.
[2]	R. Ramaraj, D. D-W. Tsai, P. H. Chen, “Chlorophyll is not accurate measurement for algal biomass”, Chiang Mai Journal of Science, 2013, 40: 547–555.
[3]	R. Ramaraj, D. D-W. Tsai, P. H. Chen, “An exploration of the relationships between microalgae biomass growth and related environmental variables”, Journal of Photochemistry and Photobiology B: Biology, 2014, 135: 44–47.
[4]	R. Ramaraj, D. D-W. Tsai, P. H. Chen, “Freshwater microalgae niche of air carbon dioxide mitigation”, Ecological Engineering, 2014; 68: 47–52.
[5]	A. Demirbas, “Production of biodiesel from algae oils”, Energy Conversation Management, 2009, 50: 4–34.
[6]	DOE, “Recovery and Sequestration of CO2 from Stationary Combustion Systems by Photosynthesis of Microalgae”, Office of Fossil Energy National Energy Technology Laboratory, U.S. Department of Energy, 2006.
[7]	H. J. Ryu, K. K. Oh, Y. S. Kim, “Optimization of the influential factors for the improvement of CO2 utilization efficiency and CO2 mass transfer rate”, Journal of Industrial and Engineering Chemistry, 2009, 15: 471–475.
[8]	C. N. Dasgupta, J. Gilbert, P. Lindblad, T. Heidorn, S. A. Borgvang, K. Skjånes, D. Das, “Recent trends on the development of photobiological processes for the improvement of hydrogen production”, International Journal of Hydrogen Energy, 2010, 35: 10218–10238.
[9]	R. Ramaraj, “Freshwater microalgae growth and Carbon dioxide Sequestration”, Taichung, Taiwan, National Chung Hsing University, PhD thesis, 2013.
[10]	W. J. Oswald, H. B. Gotaas, H. F. Ludwig, “Algae symbiosis in oxidation ponds II. Growth characteristics of Chlorella pyrenoidosa cultured in sewage”, Sewage and Industrial Wastes, 1953, 25: 26-37.
[11]	W. J. Oswald, “The coming industry of controlled photosynthesis”, American Journal of Public Health ,1962, 52: 235–242.
[12]	W. J. Oswald, “Microalgae and wastewater treatment”, Borowitzka and Borowitzka ed., Cambridge University Press, UK. 1988.
[13]	W. J. Oswald, “My sixty years in applied algology”, Journal of Applied Phycology, 2003, 15: 99–106.
[14]	R. Kothari, V. V. Pathak, V. Kumar, D. P. Singh, “Experimental study for growth potential of unicellular alga Chlorella pyrenoidosa on dairy waste water: an integrated approach for treatment and biofuel production”, Bioresource Technology, 2012, 116, 466–470.
[15]	S. Mandal, N. Mallick, “Waste Utilization and Biodiesel Production by the Green Microalga Scenedesmus obliquus” Applied and Environmental Microbiology, 2011, 77: 374–377
[16]	A. C. Wilkie, W. W. Mulbry, 2002 “Recovery of dairy manure nutrients by benthic freshwater algae”, Bioresource Technology, 84: 81–91.
[17]	M. S. Kumar, Z. H. Miao, S. K. Wyatt, “Inﬂuence of nutrient loads, feeding frequency and inoculum source on growth of Chlorella vulgaris in digested piggery effluent culture medium” Bioresource Technology, 2010, 101: 6012–6018.
[18]	N. Rashid, M. S. U. Rehman, S. Memon, Z. U. Rahman, K. Lee, J.-In Hana, “Current status, barriers and developments in biohydrogen production by microalgae”, Renewable and Sustainable Energy Reviews, 2013, 22: 571–579.
[19]	D. Das, T. N. Veziroǧlu, “Hydrogen production by biological processes: a survey of literature”, International Journal of Hydrogen Energy, 2001, 26: 13–28.
[20]	B. K. Nayak, S. Roy, D. Das, “The potential of sustainable algal biofuel production using wastewater resources”, International Journal of Hydrogen Energy, 2014, 39: 7553–7560.
[21]	J. Komárek, V. Jankovská, “Review of the green algal genus Pediastrum: Implication for pollen-analytical research”, Bibliotheca Phycologica, 2001, 108: 1-127.
[22]	H. W. Bischoff, C. H. Bold, “Phycological studies. IV. Some soil algae from enchanted rock and related algal species”, University of Texas Publication, 1963; 6318: 32–36.
[23]	S. Kant, P. Gupta, “Algal Flora of Ladakh”, Scientific Publishers, Jodhpur, India, 1998, p.341.
[24]	APHA, AWWA, WPCF, “Standards Methods for the Examination of Water and Wastewater”, 21st ed. APHA-AWWA-WPCF, Washington, DC, 2005.
[25]	S. Kosourov, A. Tsygankov, M. Seibert, M. L. Ghirardi, “Sustained hydrogen photoproduction by Chlamydomonas reinhardtii: effects of culture parameters”, Biotechnology and Bioengineering, 2002, 78: 731–740.
[26]	M. Ni, D. Y. C. Leung, M.K.H. Leung, K. Sumathy, “An overview of hydrogen production from biomass”, Fuel Processing Technology, 2006, 87: 461–472.
[27]	A. Melis, T. Happe, “Hydrogen Production. Green Algae as a Source of Energy”, Plant Physiology, 127: 740–748.
[28]	G. Torzillo, A. Scoma, C. Faralon, L. Gianelli, “Advances in the biotechnology of hydrogen production with the microalga Chlamydomonas reinhardtii”, Critical Reviews in Biotechnology, 2014, 1–12, DOI: 10.3109/07388551.2014.90073

Cite This Article

Plain Text BibTeX RIS

APA Style

Ramaraj Rameshprabu, Rungthip Kawaree, Yuwalee Unpaprom. (2015). A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production. International Journal of Sustainable and Green Energy, 4(1-1), 7-12. https://doi.org/10.11648/j.ijrse.s.2015040101.12

Copy | Download

ACS Style

Ramaraj Rameshprabu; Rungthip Kawaree; Yuwalee Unpaprom. A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production. Int. J. Sustain. Green Energy 2015, 4(1-1), 7-12. doi: 10.11648/j.ijrse.s.2015040101.12

Copy | Download

AMA Style

Ramaraj Rameshprabu, Rungthip Kawaree, Yuwalee Unpaprom. A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production. Int J Sustain Green Energy. 2015;4(1-1):7-12. doi: 10.11648/j.ijrse.s.2015040101.12

Copy | Download

@article{10.11648/j.ijrse.s.2015040101.12,
  author = {Ramaraj Rameshprabu and Rungthip Kawaree and Yuwalee Unpaprom},
  title = {A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production},
  journal = {International Journal of Sustainable and Green Energy},
  volume = {4},
  number = {1-1},
  pages = {7-12},
  doi = {10.11648/j.ijrse.s.2015040101.12},
  url = {https://doi.org/10.11648/j.ijrse.s.2015040101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.s.2015040101.12},
  abstract = {Biofuels are gaining attention worldwide as a way to reduce the dependence on fossil fuels. Biological Hydrogen (H2) production is considered the most environmentally friendly route of producing H2, fulﬁlling the goals of recycling renewable resources and producing clean energy. It has attracted global attention because of its potential to become an inexhaustible, low cost, renewable source of clean energy and appears as an alternative fuel. H2 production processes offer a technique through which renewable energy sources like biomass can be utilized for the generation of the cleanest energy carrier for the use of mankind. This paper presents laboratory results of biological production of hydrogen by green alga was isolated from fresh water fish pond in Sansai, Chiang Mai province, Thailand. Under light microscope, this green alga was identified as belonging to the genus Pediastrum and species P. duplex Meyen. The successful culture was established and grown in poultry litter effluent medium (PLEM) under a light intensity of 37.5 μmol-1m2 sec-1 and a temperature of 25°C. The nutrient requirements and process conditions that encourage the growth of dense and healthy algal cultures were explored. The highest H2 was produced when cultivated cells in PLEM for 21 hours under light and then incubated under anaerobic adaptation for 4 hours.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production
AU  - Ramaraj Rameshprabu
AU  - Rungthip Kawaree
AU  - Yuwalee Unpaprom
Y1  - 2015/01/11
PY  - 2015
N1  - https://doi.org/10.11648/j.ijrse.s.2015040101.12
DO  - 10.11648/j.ijrse.s.2015040101.12
T2  - International Journal of Sustainable and Green Energy
JF  - International Journal of Sustainable and Green Energy
JO  - International Journal of Sustainable and Green Energy
SP  - 7
EP  - 12
PB  - Science Publishing Group
SN  - 2575-1549
UR  - https://doi.org/10.11648/j.ijrse.s.2015040101.12
AB  - Biofuels are gaining attention worldwide as a way to reduce the dependence on fossil fuels. Biological Hydrogen (H2) production is considered the most environmentally friendly route of producing H2, fulﬁlling the goals of recycling renewable resources and producing clean energy. It has attracted global attention because of its potential to become an inexhaustible, low cost, renewable source of clean energy and appears as an alternative fuel. H2 production processes offer a technique through which renewable energy sources like biomass can be utilized for the generation of the cleanest energy carrier for the use of mankind. This paper presents laboratory results of biological production of hydrogen by green alga was isolated from fresh water fish pond in Sansai, Chiang Mai province, Thailand. Under light microscope, this green alga was identified as belonging to the genus Pediastrum and species P. duplex Meyen. The successful culture was established and grown in poultry litter effluent medium (PLEM) under a light intensity of 37.5 μmol-1m2 sec-1 and a temperature of 25°C. The nutrient requirements and process conditions that encourage the growth of dense and healthy algal cultures were explored. The highest H2 was produced when cultivated cells in PLEM for 21 hours under light and then incubated under anaerobic adaptation for 4 hours.
VL  - 4
IS  - 1-1
ER  -

Copy | Download

Author Information

Ramaraj Rameshprabu

School of Renewable Energy, Maejo University, Sansai, Chiang Mai-50290, Thailand
Rungthip Kawaree

Program in Biotechnology, Faculty of Science, Maejo University, Sansai, Chiang Mai-50290, Thailand
Yuwalee Unpaprom

Program in Biotechnology, Faculty of Science, Maejo University, Sansai, Chiang Mai-50290, Thailand

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Ramaraj Rameshprabu, Rungthip Kawaree, Yuwalee Unpaprom. (2015). A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production. International Journal of Sustainable and Green Energy, 4(1-1), 7-12. https://doi.org/10.11648/j.ijrse.s.2015040101.12

Copy | Download

ACS Style

Ramaraj Rameshprabu; Rungthip Kawaree; Yuwalee Unpaprom. A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production. Int. J. Sustain. Green Energy 2015, 4(1-1), 7-12. doi: 10.11648/j.ijrse.s.2015040101.12

Copy | Download

AMA Style

Ramaraj Rameshprabu, Rungthip Kawaree, Yuwalee Unpaprom. A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production. Int J Sustain Green Energy. 2015;4(1-1):7-12. doi: 10.11648/j.ijrse.s.2015040101.12

Copy | Download

@article{10.11648/j.ijrse.s.2015040101.12,
  author = {Ramaraj Rameshprabu and Rungthip Kawaree and Yuwalee Unpaprom},
  title = {A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production},
  journal = {International Journal of Sustainable and Green Energy},
  volume = {4},
  number = {1-1},
  pages = {7-12},
  doi = {10.11648/j.ijrse.s.2015040101.12},
  url = {https://doi.org/10.11648/j.ijrse.s.2015040101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijrse.s.2015040101.12},
  abstract = {Biofuels are gaining attention worldwide as a way to reduce the dependence on fossil fuels. Biological Hydrogen (H2) production is considered the most environmentally friendly route of producing H2, fulﬁlling the goals of recycling renewable resources and producing clean energy. It has attracted global attention because of its potential to become an inexhaustible, low cost, renewable source of clean energy and appears as an alternative fuel. H2 production processes offer a technique through which renewable energy sources like biomass can be utilized for the generation of the cleanest energy carrier for the use of mankind. This paper presents laboratory results of biological production of hydrogen by green alga was isolated from fresh water fish pond in Sansai, Chiang Mai province, Thailand. Under light microscope, this green alga was identified as belonging to the genus Pediastrum and species P. duplex Meyen. The successful culture was established and grown in poultry litter effluent medium (PLEM) under a light intensity of 37.5 μmol-1m2 sec-1 and a temperature of 25°C. The nutrient requirements and process conditions that encourage the growth of dense and healthy algal cultures were explored. The highest H2 was produced when cultivated cells in PLEM for 21 hours under light and then incubated under anaerobic adaptation for 4 hours.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - A Newly Isolated Green Alga, Pediastrum Duplex Meyen, from Thailand with Efficient Hydrogen Production
AU  - Ramaraj Rameshprabu
AU  - Rungthip Kawaree
AU  - Yuwalee Unpaprom
Y1  - 2015/01/11
PY  - 2015
N1  - https://doi.org/10.11648/j.ijrse.s.2015040101.12
DO  - 10.11648/j.ijrse.s.2015040101.12
T2  - International Journal of Sustainable and Green Energy
JF  - International Journal of Sustainable and Green Energy
JO  - International Journal of Sustainable and Green Energy
SP  - 7
EP  - 12
PB  - Science Publishing Group
SN  - 2575-1549
UR  - https://doi.org/10.11648/j.ijrse.s.2015040101.12
AB  - Biofuels are gaining attention worldwide as a way to reduce the dependence on fossil fuels. Biological Hydrogen (H2) production is considered the most environmentally friendly route of producing H2, fulﬁlling the goals of recycling renewable resources and producing clean energy. It has attracted global attention because of its potential to become an inexhaustible, low cost, renewable source of clean energy and appears as an alternative fuel. H2 production processes offer a technique through which renewable energy sources like biomass can be utilized for the generation of the cleanest energy carrier for the use of mankind. This paper presents laboratory results of biological production of hydrogen by green alga was isolated from fresh water fish pond in Sansai, Chiang Mai province, Thailand. Under light microscope, this green alga was identified as belonging to the genus Pediastrum and species P. duplex Meyen. The successful culture was established and grown in poultry litter effluent medium (PLEM) under a light intensity of 37.5 μmol-1m2 sec-1 and a temperature of 25°C. The nutrient requirements and process conditions that encourage the growth of dense and healthy algal cultures were explored. The highest H2 was produced when cultivated cells in PLEM for 21 hours under light and then incubated under anaerobic adaptation for 4 hours.
VL  - 4
IS  - 1-1
ER  -

Copy | Download