Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of <i>Eribroma oblongum </i> (Malvaceae) Stem Bark

Tsague M. V.; Fokunang Ntungwen C.; Talla E.; Djekilamber A.; Tembe-Fokunang E. A.; Ngo Lemba Tom E.; Essomba C.; Ntchapda F.; Sokeng Dongmo S.; Oben Enyong J.; Ze Minkande J.; Afane Ela A.; Temdie Guemmogne R. J.; Dimo T.; Chi Fru G.; Ngadjui Tchaleu B.

doi:doi:10.11648/j.jdmp.20160206.13

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark

Tsague M. V., Fokunang Ntungwen C., Talla E., Djekilamber A., Tembe-Fokunang E. A., Ngo Lemba Tom E., Essomba C., Ntchapda F., Sokeng Dongmo S., Oben Enyong J., Ze Minkande J., Afane Ela A., Temdie Guemmogne R. J., Dimo T., Chi Fru G., Ngadjui Tchaleu B.

Published in Journal of Diseases and Medicinal Plants (Volume 2, Issue 6)

Received: 6 October 2016 Accepted: 14 October 2016 Published: 21 November 2016

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Abstract

The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum on arterial blood pressure and heart rate in normotensive rat (NTR) and their mechanisms of EAEO. The effects of ethyl acetate extract of Eribroma oblongum (EAEO; 10, 20, 30 mg/kg; i.v) was tested on systolic blood pressure (SBP) and heart rate (HR) of normotensive rat. The mechanism of EAEO (20mg/kg) was studied in the presence of atropine, yohimbine, propranolol, L-NAME or reserpine. At the end of the experiment, SBP and HR were recorded. EAEO (10-20 mg/kg) induced a significant hypotensive effect of SBP. The hypotensive effects of EAEO (20 mg/kg) were inhibited by pre-treatment of rats with atropine, reserpine, yohimbine and L-NAME. At the end of this study the result demonstrates that the hypotensive as well as the antihypertensive effects of the ethyl acetate extract of the stem bark of Eribroma oblongum. Our data validate the use of the extract in traditional medicine against hypertension. The effect on blood pressure is, at least in part, due to a modulation of the orthosympathetic nervous system and to the improvement of the antioxidant status. Further studies are from now needed to study the toxicity of Eribroma oblongum.

Published in	Journal of Diseases and Medicinal Plants (Volume 2, Issue 6)
DOI	10.11648/j.jdmp.20160206.13
Page(s)	74-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Eribroma oblongum, Betulinic Acid, Hypotensive Effect, Wistar Rats

References

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Tsague M. V., Fokunang Ntungwen C., Talla E., Djekilamber A., Tembe-Fokunang E. A., et al. (2016). Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark. Journal of Diseases and Medicinal Plants, 2(6), 74-82. https://doi.org/10.11648/j.jdmp.20160206.13

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ACS Style

Tsague M. V.; Fokunang Ntungwen C.; Talla E.; Djekilamber A.; Tembe-Fokunang E. A., et al. Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark. J. Dis. Med. Plants 2016, 2(6), 74-82. doi: 10.11648/j.jdmp.20160206.13

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AMA Style

Tsague M. V., Fokunang Ntungwen C., Talla E., Djekilamber A., Tembe-Fokunang E. A., et al. Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark. J Dis Med Plants. 2016;2(6):74-82. doi: 10.11648/j.jdmp.20160206.13

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@article{10.11648/j.jdmp.20160206.13,
  author = {Tsague M. V. and Fokunang Ntungwen C. and Talla E. and Djekilamber A. and Tembe-Fokunang E. A. and Ngo Lemba Tom E. and Essomba C. and Ntchapda F. and Sokeng Dongmo S. and Oben Enyong J. and Ze Minkande J. and Afane Ela A. and Temdie Guemmogne R. J. and Dimo T. and Chi Fru G. and Ngadjui Tchaleu B.},
  title = {Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum  (Malvaceae) Stem Bark},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {2},
  number = {6},
  pages = {74-82},
  doi = {10.11648/j.jdmp.20160206.13},
  url = {https://doi.org/10.11648/j.jdmp.20160206.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20160206.13},
  abstract = {The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum on arterial blood pressure and heart rate in normotensive rat (NTR) and their mechanisms of EAEO. The effects of ethyl acetate extract of Eribroma oblongum (EAEO; 10, 20, 30 mg/kg; i.v) was tested on systolic blood pressure (SBP) and heart rate (HR) of normotensive rat. The mechanism of EAEO (20mg/kg) was studied in the presence of atropine, yohimbine, propranolol, L-NAME or reserpine. At the end of the experiment, SBP and HR were recorded. EAEO (10-20 mg/kg) induced a significant hypotensive effect of SBP. The hypotensive effects of EAEO (20 mg/kg) were inhibited by pre-treatment of rats with atropine, reserpine, yohimbine and L-NAME. At the end of this study the result demonstrates that the hypotensive as well as the antihypertensive effects of the ethyl acetate extract of the stem bark of Eribroma oblongum. Our data validate the use of the extract in traditional medicine against hypertension. The effect on blood pressure is, at least in part, due to a modulation of the orthosympathetic nervous system and to the improvement of the antioxidant status. Further studies are from now needed to study the toxicity of Eribroma oblongum.},
 year = {2016}
}

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TY - JOUR
T1 - Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark
AU - Tsague M. V.
AU - Fokunang Ntungwen C.
AU - Talla E.
AU - Djekilamber A.
AU - Tembe-Fokunang E. A.
AU - Ngo Lemba Tom E.
AU - Essomba C.
AU - Ntchapda F.
AU - Sokeng Dongmo S.
AU - Oben Enyong J.
AU - Ze Minkande J.
AU - Afane Ela A.
AU - Temdie Guemmogne R. J.
AU - Dimo T.
AU - Chi Fru G.
AU - Ngadjui Tchaleu B.
Y1 - 2016/11/21
PY - 2016
N1 - https://doi.org/10.11648/j.jdmp.20160206.13
DO - 10.11648/j.jdmp.20160206.13
T2 - Journal of Diseases and Medicinal Plants
JF - Journal of Diseases and Medicinal Plants
JO - Journal of Diseases and Medicinal Plants
SP - 74
EP - 82
PB - Science Publishing Group
SN - 2469-8210
UR - https://doi.org/10.11648/j.jdmp.20160206.13
AB - The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum on arterial blood pressure and heart rate in normotensive rat (NTR) and their mechanisms of EAEO. The effects of ethyl acetate extract of Eribroma oblongum (EAEO; 10, 20, 30 mg/kg; i.v) was tested on systolic blood pressure (SBP) and heart rate (HR) of normotensive rat. The mechanism of EAEO (20mg/kg) was studied in the presence of atropine, yohimbine, propranolol, L-NAME or reserpine. At the end of the experiment, SBP and HR were recorded. EAEO (10-20 mg/kg) induced a significant hypotensive effect of SBP. The hypotensive effects of EAEO (20 mg/kg) were inhibited by pre-treatment of rats with atropine, reserpine, yohimbine and L-NAME. At the end of this study the result demonstrates that the hypotensive as well as the antihypertensive effects of the ethyl acetate extract of the stem bark of Eribroma oblongum. Our data validate the use of the extract in traditional medicine against hypertension. The effect on blood pressure is, at least in part, due to a modulation of the orthosympathetic nervous system and to the improvement of the antioxidant status. Further studies are from now needed to study the toxicity of Eribroma oblongum.
VL - 2
IS - 6
ER -

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Author Information

Tsague M. V.

Department of Surgery and Specialties Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Fokunang Ntungwen C.

Department of Pharmaco-Toxicology and pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Talla E.

Department of Chemistry, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Djekilamber A.

Department of Chemistry, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon
Tembe-Fokunang E. A.

Department of Pharmaco-Toxicology and pharmacokinetics, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Ngo Lemba Tom E.

Department of Biology, Higher Teachers’ Training College, University of Yaoundé I, Yaounde, Cameroon
Essomba C.

Department of Biology, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon
Ntchapda F.

Department of Biological Sciences, Faculty of Sciences, Ngaoundere, Ngaoundere, Cameroon
Sokeng Dongmo S.

Department of Biological Sciences, Faculty of Sciences, Ngaoundere, Ngaoundere, Cameroon
Oben Enyong J.

Department of Biochemistry, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon
Ze Minkande J.

Department of Surgery and Specialties Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Afane Ela A.

Department of Surgery and Specialties Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Temdie Guemmogne R. J.

Department of Biological Sciences, Faculty of Sciences, Ngaoundere, Ngaoundere, Cameroon
Dimo T.

Department of Biology, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon
Chi Fru G.

Department of Organic Chemistry, Faculty of Sciences, University of Yaounde I, Yaounde, Cameroon
Ngadjui Tchaleu B.

Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon

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Tsague M. V., Fokunang Ntungwen C., Talla E., Djekilamber A., Tembe-Fokunang E. A., et al. (2016). Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark. Journal of Diseases and Medicinal Plants, 2(6), 74-82. https://doi.org/10.11648/j.jdmp.20160206.13

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Tsague M. V.; Fokunang Ntungwen C.; Talla E.; Djekilamber A.; Tembe-Fokunang E. A., et al. Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark. J. Dis. Med. Plants 2016, 2(6), 74-82. doi: 10.11648/j.jdmp.20160206.13

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AMA Style

Tsague M. V., Fokunang Ntungwen C., Talla E., Djekilamber A., Tembe-Fokunang E. A., et al. Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark. J Dis Med Plants. 2016;2(6):74-82. doi: 10.11648/j.jdmp.20160206.13

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@article{10.11648/j.jdmp.20160206.13,
  author = {Tsague M. V. and Fokunang Ntungwen C. and Talla E. and Djekilamber A. and Tembe-Fokunang E. A. and Ngo Lemba Tom E. and Essomba C. and Ntchapda F. and Sokeng Dongmo S. and Oben Enyong J. and Ze Minkande J. and Afane Ela A. and Temdie Guemmogne R. J. and Dimo T. and Chi Fru G. and Ngadjui Tchaleu B.},
  title = {Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum  (Malvaceae) Stem Bark},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {2},
  number = {6},
  pages = {74-82},
  doi = {10.11648/j.jdmp.20160206.13},
  url = {https://doi.org/10.11648/j.jdmp.20160206.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20160206.13},
  abstract = {The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum on arterial blood pressure and heart rate in normotensive rat (NTR) and their mechanisms of EAEO. The effects of ethyl acetate extract of Eribroma oblongum (EAEO; 10, 20, 30 mg/kg; i.v) was tested on systolic blood pressure (SBP) and heart rate (HR) of normotensive rat. The mechanism of EAEO (20mg/kg) was studied in the presence of atropine, yohimbine, propranolol, L-NAME or reserpine. At the end of the experiment, SBP and HR were recorded. EAEO (10-20 mg/kg) induced a significant hypotensive effect of SBP. The hypotensive effects of EAEO (20 mg/kg) were inhibited by pre-treatment of rats with atropine, reserpine, yohimbine and L-NAME. At the end of this study the result demonstrates that the hypotensive as well as the antihypertensive effects of the ethyl acetate extract of the stem bark of Eribroma oblongum. Our data validate the use of the extract in traditional medicine against hypertension. The effect on blood pressure is, at least in part, due to a modulation of the orthosympathetic nervous system and to the improvement of the antioxidant status. Further studies are from now needed to study the toxicity of Eribroma oblongum.},
 year = {2016}
}

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