Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia

Gédéon Bongo; Huruma Tuntufye; Koto-te-Nyiwa Ngbolua; Joseph Malakalinga; Tshiama Claudine; Aaron Pambu; Fabrice Mwanza; Clément Mbadiko; Gisèle Makengo; Jeff Iteku; Dorothée Tshilanda; Pius Mpiana; Théophile Mbemba; Rudovick Kazwala

doi:doi:10.11648/j.jdmp.20170305.12

Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia

Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, Aaron Pambu, Fabrice Mwanza, Clément Mbadiko, Gisèle Makengo, Jeff Iteku, Dorothée Tshilanda, Pius Mpiana, Théophile Mbemba, Rudovick Kazwala

Published in Journal of Diseases and Medicinal Plants (Volume 3, Issue 5)

Received: 6 October 2017 Accepted: 28 October 2017 Published: 30 November 2017

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Abstract

Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp. and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.

Published in	Journal of Diseases and Medicinal Plants (Volume 3, Issue 5)
DOI	10.11648/j.jdmp.20170305.12
Page(s)	88-96
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Tuberculosis, Lowenstein Jensen, Anti-mycobacterial Activity, Phytochemicals, Medicinal Plants

References

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APA Style

Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, et al. (2017). Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. Journal of Diseases and Medicinal Plants, 3(5), 88-96. https://doi.org/10.11648/j.jdmp.20170305.12

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ACS Style

Gédéon Bongo; Huruma Tuntufye; Koto-te-Nyiwa Ngbolua; Joseph Malakalinga; Tshiama Claudine, et al. Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. J. Dis. Med. Plants 2017, 3(5), 88-96. doi: 10.11648/j.jdmp.20170305.12

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AMA Style

Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, et al. Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. J Dis Med Plants. 2017;3(5):88-96. doi: 10.11648/j.jdmp.20170305.12

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@article{10.11648/j.jdmp.20170305.12,
  author = {Gédéon Bongo and Huruma Tuntufye and Koto-te-Nyiwa Ngbolua and Joseph Malakalinga and Tshiama Claudine and Aaron Pambu and Fabrice Mwanza and Clément Mbadiko and Gisèle Makengo and Jeff Iteku and Dorothée Tshilanda and Pius Mpiana and Théophile Mbemba and Rudovick Kazwala},
  title = {Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {3},
  number = {5},
  pages = {88-96},
  doi = {10.11648/j.jdmp.20170305.12},
  url = {https://doi.org/10.11648/j.jdmp.20170305.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170305.12},
  abstract = {Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp.  and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.},
 year = {2017}
}

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TY  - JOUR
T1  - Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia
AU  - Gédéon Bongo
AU  - Huruma Tuntufye
AU  - Koto-te-Nyiwa Ngbolua
AU  - Joseph Malakalinga
AU  - Tshiama Claudine
AU  - Aaron Pambu
AU  - Fabrice Mwanza
AU  - Clément Mbadiko
AU  - Gisèle Makengo
AU  - Jeff Iteku
AU  - Dorothée Tshilanda
AU  - Pius Mpiana
AU  - Théophile Mbemba
AU  - Rudovick Kazwala
Y1  - 2017/11/30
PY  - 2017
N1  - https://doi.org/10.11648/j.jdmp.20170305.12
DO  - 10.11648/j.jdmp.20170305.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 88
EP  - 96
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20170305.12
AB  - Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp.  and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.
VL  - 3
IS  - 5
ER  -

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Author Information

Gédéon Bongo

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Huruma Tuntufye

Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
Koto-te-Nyiwa Ngbolua

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Joseph Malakalinga

Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
Tshiama Claudine

Enseignement et Administration en Soins Infirmiers, Section Sciences Infirmières, Institut Supérieur des Techniques Médicales, Kinshasa, Democratic Republic of the Congo
Aaron Pambu

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Fabrice Mwanza

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Clément Mbadiko

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Gisèle Makengo

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Jeff Iteku

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Dorothée Tshilanda

Department of Chemistry, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Pius Mpiana

Department of Chemistry, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Théophile Mbemba

Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo
Rudovick Kazwala

Department of Veterinary Medicine and Public Health, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania

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APA Style

Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, et al. (2017). Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. Journal of Diseases and Medicinal Plants, 3(5), 88-96. https://doi.org/10.11648/j.jdmp.20170305.12

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ACS Style

Gédéon Bongo; Huruma Tuntufye; Koto-te-Nyiwa Ngbolua; Joseph Malakalinga; Tshiama Claudine, et al. Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. J. Dis. Med. Plants 2017, 3(5), 88-96. doi: 10.11648/j.jdmp.20170305.12

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AMA Style

Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, et al. Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. J Dis Med Plants. 2017;3(5):88-96. doi: 10.11648/j.jdmp.20170305.12

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@article{10.11648/j.jdmp.20170305.12,
  author = {Gédéon Bongo and Huruma Tuntufye and Koto-te-Nyiwa Ngbolua and Joseph Malakalinga and Tshiama Claudine and Aaron Pambu and Fabrice Mwanza and Clément Mbadiko and Gisèle Makengo and Jeff Iteku and Dorothée Tshilanda and Pius Mpiana and Théophile Mbemba and Rudovick Kazwala},
  title = {Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {3},
  number = {5},
  pages = {88-96},
  doi = {10.11648/j.jdmp.20170305.12},
  url = {https://doi.org/10.11648/j.jdmp.20170305.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170305.12},
  abstract = {Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp.  and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia
AU  - Gédéon Bongo
AU  - Huruma Tuntufye
AU  - Koto-te-Nyiwa Ngbolua
AU  - Joseph Malakalinga
AU  - Tshiama Claudine
AU  - Aaron Pambu
AU  - Fabrice Mwanza
AU  - Clément Mbadiko
AU  - Gisèle Makengo
AU  - Jeff Iteku
AU  - Dorothée Tshilanda
AU  - Pius Mpiana
AU  - Théophile Mbemba
AU  - Rudovick Kazwala
Y1  - 2017/11/30
PY  - 2017
N1  - https://doi.org/10.11648/j.jdmp.20170305.12
DO  - 10.11648/j.jdmp.20170305.12
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 88
EP  - 96
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20170305.12
AB  - Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp.  and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.
VL  - 3
IS  - 5
ER  -

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