Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers

Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya; Jayantkumar G. Talati

doi:doi:10.11648/j.jps.20150304.14

Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, Jayantkumar G. Talati

Published in Journal of Plant Sciences (Volume 3, Issue 4)

Received: 27 June 2015 Accepted: 4 July 2015 Published: 14 July 2015

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Abstract

Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.

Published in	Journal of Plant Sciences (Volume 3, Issue 4)
DOI	10.11648/j.jps.20150304.14
Page(s)	191-196
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Cold-Induced Sweetening, Potato, Solanum tuberosum, SSR, Genetic Diversity

References

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APA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. (2015). Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. Journal of Plant Sciences, 3(4), 191-196. https://doi.org/10.11648/j.jps.20150304.14

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ACS Style

Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J. Plant Sci. 2015, 3(4), 191-196. doi: 10.11648/j.jps.20150304.14

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AMA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J Plant Sci. 2015;3(4):191-196. doi: 10.11648/j.jps.20150304.14

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@article{10.11648/j.jps.20150304.14,
  author = {Galani Yamdeu Joseph Hubert and Pooja H. Gupta and Nilesh J. Patel and Avadh K. Shah and Rajeshkumar R. Acharya and Jayantkumar G. Talati},
  title = {Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {4},
  pages = {191-196},
  doi = {10.11648/j.jps.20150304.14},
  url = {https://doi.org/10.11648/j.jps.20150304.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150304.14},
  abstract = {Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.},
 year = {2015}
}

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TY - JOUR
T1 - Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers
AU - Galani Yamdeu Joseph Hubert
AU - Pooja H. Gupta
AU - Nilesh J. Patel
AU - Avadh K. Shah
AU - Rajeshkumar R. Acharya
AU - Jayantkumar G. Talati
Y1 - 2015/07/14
PY - 2015
N1 - https://doi.org/10.11648/j.jps.20150304.14
DO - 10.11648/j.jps.20150304.14
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 191
EP - 196
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20150304.14
AB - Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.
VL - 3
IS - 4
ER -

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Author Information

Galani Yamdeu Joseph Hubert

Department of Agriculture and Veterinary Medicine, Université des Montagnes, Bangangté, Cameroon
Pooja H. Gupta

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Nilesh J. Patel

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Avadh K. Shah

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India
Rajeshkumar R. Acharya

Main Vegetable Research Station, Anand Agricultural University, Gujarat, India
Jayantkumar G. Talati

Department of Biochemistry, B.A. College of Agriculture, Anand Agricultural University, Gujarat, India

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APA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. (2015). Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. Journal of Plant Sciences, 3(4), 191-196. https://doi.org/10.11648/j.jps.20150304.14

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ACS Style

Galani Yamdeu Joseph Hubert; Pooja H. Gupta; Nilesh J. Patel; Avadh K. Shah; Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J. Plant Sci. 2015, 3(4), 191-196. doi: 10.11648/j.jps.20150304.14

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AMA Style

Galani Yamdeu Joseph Hubert, Pooja H. Gupta, Nilesh J. Patel, Avadh K. Shah, Rajeshkumar R. Acharya, et al. Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers. J Plant Sci. 2015;3(4):191-196. doi: 10.11648/j.jps.20150304.14

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@article{10.11648/j.jps.20150304.14,
  author = {Galani Yamdeu Joseph Hubert and Pooja H. Gupta and Nilesh J. Patel and Avadh K. Shah and Rajeshkumar R. Acharya and Jayantkumar G. Talati},
  title = {Molecular Characterization of Indian Potato (Solanum tuberosum L.) Varieties for Cold-Induced Sweetening Using SSR Markers},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {4},
  pages = {191-196},
  doi = {10.11648/j.jps.20150304.14},
  url = {https://doi.org/10.11648/j.jps.20150304.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150304.14},
  abstract = {Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs.},
 year = {2015}
}

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