Experimental studies explored the antidiabetic and thrombolytic effect of several Spilanthes species in various animal models, but previously no study was conducted to establish the antidiabetic and thrombolytic potentiality of Spilanthes paniculata. The present study investigate the antidiabetic and thrombolytic effects of ethanolic extract of Spilanthes paniculata leaves with the intention to find the drug for diabetes and thrombosis management from natural sources. The hypoglycemic effect of the extracts was tested in normal and alloxan-induced diabetic mice. Blood glucose level was measured according to glucose oxidase method. The thrombolytic activity was assessed by using human erythrocyte and the results were compared with standard streptokinase (SK). In the present research, the ethanolic extract of Spilanthes paniculata reduces the blood glucose level at a dose and time dependant manner. It was observed that the plant possess significant antidiabetic activity (P<0.05) at higher dose (450 mg/kg body weight) when compared with standard drug glibenclamide. The extract, at a dose of 150, 300 and 450 mg/kg body weight showed glucose reduction from 23.37±1.80, 20.1±2.60 and 17.13±1.36 initial levels to 11.07±1.98, 10.1±0.26 and 8.3±0.15 mmol/L after 8 hours respectively. In this study, the ethanolic extract of Spilanthes paniculata showed moderate clot lysis activity. The clot lysis activity of control, standard (streptokinase) and ethanolic extract of Spilanthes paniculata was 2.65%, 93.35% and 46.78% respectively. This study explored that ethanolic extract of Spilanthes paniculata leaves has potential antidiabetic and moderate thrombolytic activity.
| Published in |
Journal of Plant Sciences (Volume 2, Issue 6-1)
This article belongs to the Special Issue Pharmacological and Biological Investigation of Medicinal Plants |
| DOI | 10.11648/j.jps.s.2014020601.13 |
| Page(s) | 13-18 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Spilanthes paniculata, Antidiabetic, Alloxan, Sptreptokinase, Thrombolytic
| [1] | R.K. Jansen, “ Syst Bot Monogr,” 1985, 8, p.110-5. |
| [2] | V. Pandey, V. Agrawal, K. Raghavendra and A.P. Das, “Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria ( Anopheles stephensi Liston, Anopheles culicifacies , species C) and filaria vector ( Culex quinquefasciatus Say),” Parasitol Res, 2007,102, p. 171–4. |
| [3] | S. Prachayasittikul, S. Suphapong, A. Worachartcheewan, R. Lawung, S. Ruchirawat, and V. Prachayasittikul, “Bioactive metabolites from Spilanthes acmella Murr,” Molecules, 2009, 14(2), p. 850–867. |
| [4] | N. Nakatani and M. Nagashima, “Pungent alkamides from Spilanthes acmella var. oleracea Clarke,” Bioscience, Biotechnology, and Biochemistry, 1992, 56(5), p. 759–762. |
| [5] | A. A. Sittie, E .Lemmich , C.E. Olsen, L. Hviid and S. Brøgger Christensen, “Alkamides from Phyllanthus fraternus,” In: Planta Medica, 1998, 64(2), p. 192-3. |
| [6] | M. Majumder, A.K. Shukla and A. Arunachalam, “Nutrient release and fungal succession during decomposition of weed residues in a shifting cultivation system,” Communications in Biometry and Crop Science, 2008, 3(1), p. 45–59. |
| [7] | D.M. Verma, N.P. Balakrishnan and R.D. Dixit, “Flora of Madhya Pradesh,” Botanical survey of India, Kolkata, India, 1993, 1, p. 612-613. |
| [8] | Available at: http://www.novanthealth.org/Portals/92/nh_presbyterian_medical_center/documents/about_us/bulletin_boards/april_2014_nutrition_management_diabetes_english.pdf (Accessed on 14 Dec. 2014). |
| [9] | J.N. Fisher, M.N. Shahshahani and A.E. Kitabchi, “Diabetic ketoacidosis: low-dose insulin therapy by various routes,” N Engl J Med, 1977, 297, p. 238. |
| [10] | C. David Klonoff, B. John Buse, L. Loretta, Nielsen, Xuesong Guan, L. Christopher Bowlus, H. John Holcombe, E. Matthew Wintle, and David G. Maggs, “Exenatide effects on diabetes, obesity, cardiovascular risk factors and hepatic biomarkers in patients with type 2 diabetes treated for at least 3 years,” Informa healthcare, 2008, 24(1) , p. 275-286. |
| [11] | Available at: http://www.news-medical.net/health/What-is-Thrombosis.aspx9914. (Accessed on14 Dec.2014) |
| [12] | N. Alkjaersig, A.P. Fletcher and S. Sherry, “The mechanism of clot dissolution by plasmin,” J Clin Invest, 1959, 38(7), p. 1086–1095. |
| [13] | D. Collen, “Coronary thrombolysis: streptokinase or recombinanttissue-type plasminogen activator,” Annals of Internal Medicine, 1990, 112, p. 529–538. |
| [14] | A.S. Apu, F.A. Chowdhury, F. Khatun, A.T.M. Jamaluddin, A. Pathan and A. Pal, “Phytochemical Screening and In vitro Evaluation of Pharmacological Activities of Aphanamixis polystachya (Wall) Parker Fruit Extracts,” Tropical Journal of Pharmaceutical Research, 2013, 12(1), p. 111-116. |
| [15] | T. Capstick and M.T. Henry, “Efficacy of thrombolytic agents in the treatment of PE,” European Respiratory Journal, 2005, 26, p. 864-874. |
| [16] | B. Giuseppina, L .Cristiana, L .Guido, C. Piero, L.A. Antonio and R. Daniele, “Therapeutic effect of diagnostic ultrasound on enzymatic thrombolysis. An in vitro study on blood of normal subjects and patients with coronary artery disease,” Journal of Thrombosis and Haemostasis, 2004, 91, p. 1078-1083. |
| [17] | D. Collen, “ Coronary thrombolysis: streptokinase or recombinanttissue-type plasminogen activator,” Ann Intern Med, 1990, 112, p. 529–538. |
| [18] | V.J. Marder, “Recombinant streptokinase –opportunity for an improved agent,”Blood Coagul Fibrinolysis, 1993, 4, p. 1039–1040. |
| [19] | F.J. Alarcon-Aguilar, M. Jimenez-Estrada, R. Reyes-Chilpa, B. GonzalezParedes, C. Contreras-Weber, and R. Roman-Ramos, “Hypoglycemic activity of root water decoction, sesquierpenoids, and one Polysaccharide fraction from Psacalium decompositum in mice”, Journal of Ethno-Pharmacology, 2000, 69, p. 207-15. |
| [20] | S. Dhandapani, S.V. Ramasamy, S. Rajagopal and N. Namasivayam, “Hypolipidemiceffect of Cuminum cyminum L. on alloxan-induced diabetic rats,” Pharmacol. Res, 2002, 46(3), p. 251-255. |
| [21] | S. Prasad, R.S. Kashyap, J.Y. Deopujari, H.J. Purohit, G.M. Taori, H.F. Daginawala “Effect of Fagonia arabica (Dhamasa) on in vitro thrombolysis,” BMC Complement Altern Med, 2007, 7, p. 36. |
| [22] | M. Goldner and G. Gomori, “Alloxan induced diabetes,” J Endocrinol, 1943, 33, p. 297–9. |
| [23] | L. Pari and M Latha, “Antidiabetic effect of Scoparia dulcis:Effect of lipid peroxidation in Streptozotocin diabetes,” Gen. Physiol. Biophys, 2005, 24, p. 13-26. |
APA Style
Shamima Akter, Md. Ataur Rahman, Md. Abul Kalam Azad, Md. Mohiuddin, Abdullah Al Mamun, et al. (2015). Antidiabetic and Thrombolytic Effects of Ethanolic Extract of Spilanthes paniculata Leaves. Journal of Plant Sciences, 2(6-1), 13-18. https://doi.org/10.11648/j.jps.s.2014020601.13
ACS Style
Shamima Akter; Md. Ataur Rahman; Md. Abul Kalam Azad; Md. Mohiuddin; Abdullah Al Mamun, et al. Antidiabetic and Thrombolytic Effects of Ethanolic Extract of Spilanthes paniculata Leaves. J. Plant Sci. 2015, 2(6-1), 13-18. doi: 10.11648/j.jps.s.2014020601.13
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.jps.s.2014020601.13,
author = {Shamima Akter and Md. Ataur Rahman and Md. Abul Kalam Azad and Md. Mohiuddin and Abdullah Al Mamun and Jyotirmoy Sarker and Mohammad Safiqul Islam and Md. Shahid Sarwar},
title = {Antidiabetic and Thrombolytic Effects of Ethanolic Extract of Spilanthes paniculata Leaves},
journal = {Journal of Plant Sciences},
volume = {2},
number = {6-1},
pages = {13-18},
doi = {10.11648/j.jps.s.2014020601.13},
url = {https://doi.org/10.11648/j.jps.s.2014020601.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.s.2014020601.13},
abstract = {Experimental studies explored the antidiabetic and thrombolytic effect of several Spilanthes species in various animal models, but previously no study was conducted to establish the antidiabetic and thrombolytic potentiality of Spilanthes paniculata. The present study investigate the antidiabetic and thrombolytic effects of ethanolic extract of Spilanthes paniculata leaves with the intention to find the drug for diabetes and thrombosis management from natural sources. The hypoglycemic effect of the extracts was tested in normal and alloxan-induced diabetic mice. Blood glucose level was measured according to glucose oxidase method. The thrombolytic activity was assessed by using human erythrocyte and the results were compared with standard streptokinase (SK). In the present research, the ethanolic extract of Spilanthes paniculata reduces the blood glucose level at a dose and time dependant manner. It was observed that the plant possess significant antidiabetic activity (P<0.05) at higher dose (450 mg/kg body weight) when compared with standard drug glibenclamide. The extract, at a dose of 150, 300 and 450 mg/kg body weight showed glucose reduction from 23.37±1.80, 20.1±2.60 and 17.13±1.36 initial levels to 11.07±1.98, 10.1±0.26 and 8.3±0.15 mmol/L after 8 hours respectively. In this study, the ethanolic extract of Spilanthes paniculata showed moderate clot lysis activity. The clot lysis activity of control, standard (streptokinase) and ethanolic extract of Spilanthes paniculata was 2.65%, 93.35% and 46.78% respectively. This study explored that ethanolic extract of Spilanthes paniculata leaves has potential antidiabetic and moderate thrombolytic activity.},
year = {2015}
}
TY - JOUR T1 - Antidiabetic and Thrombolytic Effects of Ethanolic Extract of Spilanthes paniculata Leaves AU - Shamima Akter AU - Md. Ataur Rahman AU - Md. Abul Kalam Azad AU - Md. Mohiuddin AU - Abdullah Al Mamun AU - Jyotirmoy Sarker AU - Mohammad Safiqul Islam AU - Md. Shahid Sarwar Y1 - 2015/03/14 PY - 2015 N1 - https://doi.org/10.11648/j.jps.s.2014020601.13 DO - 10.11648/j.jps.s.2014020601.13 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 13 EP - 18 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.s.2014020601.13 AB - Experimental studies explored the antidiabetic and thrombolytic effect of several Spilanthes species in various animal models, but previously no study was conducted to establish the antidiabetic and thrombolytic potentiality of Spilanthes paniculata. The present study investigate the antidiabetic and thrombolytic effects of ethanolic extract of Spilanthes paniculata leaves with the intention to find the drug for diabetes and thrombosis management from natural sources. The hypoglycemic effect of the extracts was tested in normal and alloxan-induced diabetic mice. Blood glucose level was measured according to glucose oxidase method. The thrombolytic activity was assessed by using human erythrocyte and the results were compared with standard streptokinase (SK). In the present research, the ethanolic extract of Spilanthes paniculata reduces the blood glucose level at a dose and time dependant manner. It was observed that the plant possess significant antidiabetic activity (P<0.05) at higher dose (450 mg/kg body weight) when compared with standard drug glibenclamide. The extract, at a dose of 150, 300 and 450 mg/kg body weight showed glucose reduction from 23.37±1.80, 20.1±2.60 and 17.13±1.36 initial levels to 11.07±1.98, 10.1±0.26 and 8.3±0.15 mmol/L after 8 hours respectively. In this study, the ethanolic extract of Spilanthes paniculata showed moderate clot lysis activity. The clot lysis activity of control, standard (streptokinase) and ethanolic extract of Spilanthes paniculata was 2.65%, 93.35% and 46.78% respectively. This study explored that ethanolic extract of Spilanthes paniculata leaves has potential antidiabetic and moderate thrombolytic activity. VL - 2 IS - 6-1 ER -
Email: