<i>Asparagus racemosus</i> Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats

Abdullah Al Mamun; Mahbubul Hossain; Ariful Islam; Sonia Zaman; Md. Sahab Uddin

doi:doi:10.11648/j.plant.s.2017050501.11

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Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats

Abdullah Al Mamun, Mahbubul Hossain, Ariful Islam, Sonia Zaman, Md. Sahab Uddin

Published in Plant (Volume 5, Issue 5-1)

Received: 12 July 2016 Accepted: 18 September 2016 Published: 18 October 2016

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Abstract

Diabetes mellitus is strongly connected with changes in lipid profile and also can cause damage of several organs like liver over a long period of time. The purpose of this study was designed to evaluate the hypolipidemic and hepatoprotective effects of ethanolic root extracts of Asparagus racemosus (EEAR) Linn. alone and in combination with a lipid lowering agent (fenofibrate) in alloxan-induced diabetic rats. Diabetes was induced in male Wister albino rats by the administration of single intra-peritoneal injection of alloxan monohydrate (120 mg/kg b.w.). Two different doses of EEAR (200 and 400 mg/kg b.w.) alone, fenofibrate (30 mg/kg b.w.) and a combination of EEAR (200 mg/kg b.w.) with fenofibrate (30 mg/kg b.w.) were administered orally for the period of 14 days. After the treatment period, hypolipidemic and hepatoprotective effects were determined by examining serum biochemical markers including total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), serum glutamate oxaloacetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT) and total protein (TP) with the aid of commercially available kits. The survival rate, body weight and organ weight were also measured. The ingestion of EEAR considerably (p < 0.05, p < 0.01, p < 0.001; p < 0.05, p < 0.01) modified the activity of TC, TG, LDL, VLDL and HDL cholesterol levels when compared to the disease control and fenofibrate treated rats. The administration of combination therapy significantly (p < 0.001; p < 0.001) improved the activity of TC, TG, LDL, VLDL and HDL levels when compared to that of disease control and fenofibrate treated rats. The rats treated with EEAR markedly (p < 0.05, p < 0.01, p < 0.001; p < 0.05) reduced the level of SGOT, SGPT and TP as compared to the disease control and fenofibrate treated rats. The suggested combination therapy significantly (p < 0.001; p < 0.001) decreased the level of SGOT, SGPT and TP when compared to that of disease control and fenofibrate treated rats indicated amelioration in liver dysfunctions. The maximum survival rate was 100% found in combination therapy. During treatment period, it was observed that the considerable (p < 0.01, p < 0.001; p < 0.05, p < 0.01, p < 0.001) changes in the body weight were found in the EEAR treated rats and combination therapy on 10^th and 14^th day as compared to that of disease control and fenofibrate treated rats. In case of organs weight, the weight of the liver and weight of the pancreas were significantly (p < 0.001; p < 0.05, p < 0.01, p < 0.001) decreased in the rats treated with highest dose of EEAR (Alx+ EEAR 400) and combination therapy when compared to the disease control and fenofibrate treated rats. The current study demonstrates that combination therapy of EEAR and fenofibrate was more effective than that of monotherapy in controlling diabetes mellitus associated with cardiovascular diseases and hepatic dysfunction in alloxan-induced diabetic rats.

Published in	Plant (Volume 5, Issue 5-1) This article belongs to the Special Issue Phytotherapy
DOI	10.11648/j.plant.s.2017050501.11
Page(s)	1-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Diabetes Mellitus, Asparagus racemosus, Hypolipidemic Activity, Hepatoprotective Activity, Fenofibrate, Combination Therapy

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Abdullah Al Mamun, Mahbubul Hossain, Ariful Islam, Sonia Zaman, Md. Sahab Uddin. (2016). Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats. Plant, 5(5-1), 1-12. https://doi.org/10.11648/j.plant.s.2017050501.11

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Abdullah Al Mamun; Mahbubul Hossain; Ariful Islam; Sonia Zaman; Md. Sahab Uddin. Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats. Plant. 2016, 5(5-1), 1-12. doi: 10.11648/j.plant.s.2017050501.11

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Abdullah Al Mamun, Mahbubul Hossain, Ariful Islam, Sonia Zaman, Md. Sahab Uddin. Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats. Plant. 2016;5(5-1):1-12. doi: 10.11648/j.plant.s.2017050501.11

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@article{10.11648/j.plant.s.2017050501.11,
  author = {Abdullah Al Mamun and Mahbubul Hossain and Ariful Islam and Sonia Zaman and Md. Sahab Uddin},
  title = {Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats},
  journal = {Plant},
  volume = {5},
  number = {5-1},
  pages = {1-12},
  doi = {10.11648/j.plant.s.2017050501.11},
  url = {https://doi.org/10.11648/j.plant.s.2017050501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.s.2017050501.11},
  abstract = {Diabetes mellitus is strongly connected with changes in lipid profile and also can cause damage of several organs like liver over a long period of time. The purpose of this study was designed to evaluate the hypolipidemic and hepatoprotective effects of ethanolic root extracts of Asparagus racemosus (EEAR) Linn. alone and in combination with a lipid lowering agent (fenofibrate) in alloxan-induced diabetic rats. Diabetes was induced in male Wister albino rats by the administration of single intra-peritoneal injection of alloxan monohydrate (120 mg/kg b.w.). Two different doses of EEAR (200 and 400 mg/kg b.w.) alone, fenofibrate (30 mg/kg b.w.) and a combination of EEAR (200 mg/kg b.w.) with fenofibrate (30 mg/kg b.w.) were administered orally for the period of 14 days. After the treatment period, hypolipidemic and hepatoprotective effects were determined by examining serum biochemical markers including total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), serum glutamate oxaloacetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT) and total protein (TP) with the aid of commercially available kits. The survival rate, body weight and organ weight were also measured. The ingestion of EEAR considerably (p th and 14th day as compared to that of disease control and fenofibrate treated rats. In case of organs weight, the weight of the liver and weight of the pancreas were significantly (p < 0.001; p < 0.05, p < 0.01, p < 0.001) decreased in the rats treated with highest dose of EEAR (Alx+ EEAR 400) and combination therapy when compared to the disease control and fenofibrate treated rats. The current study demonstrates that combination therapy of EEAR and fenofibrate was more effective than that of monotherapy in controlling diabetes mellitus associated with cardiovascular diseases and hepatic dysfunction in alloxan-induced diabetic rats.},
 year = {2016}
}

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TY  - JOUR
T1  - Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats
AU  - Abdullah Al Mamun
AU  - Mahbubul Hossain
AU  - Ariful Islam
AU  - Sonia Zaman
AU  - Md. Sahab Uddin
Y1  - 2016/10/18
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.s.2017050501.11
DO  - 10.11648/j.plant.s.2017050501.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 1
EP  - 12
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.s.2017050501.11
AB  - Diabetes mellitus is strongly connected with changes in lipid profile and also can cause damage of several organs like liver over a long period of time. The purpose of this study was designed to evaluate the hypolipidemic and hepatoprotective effects of ethanolic root extracts of Asparagus racemosus (EEAR) Linn. alone and in combination with a lipid lowering agent (fenofibrate) in alloxan-induced diabetic rats. Diabetes was induced in male Wister albino rats by the administration of single intra-peritoneal injection of alloxan monohydrate (120 mg/kg b.w.). Two different doses of EEAR (200 and 400 mg/kg b.w.) alone, fenofibrate (30 mg/kg b.w.) and a combination of EEAR (200 mg/kg b.w.) with fenofibrate (30 mg/kg b.w.) were administered orally for the period of 14 days. After the treatment period, hypolipidemic and hepatoprotective effects were determined by examining serum biochemical markers including total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), serum glutamate oxaloacetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT) and total protein (TP) with the aid of commercially available kits. The survival rate, body weight and organ weight were also measured. The ingestion of EEAR considerably (p th and 14th day as compared to that of disease control and fenofibrate treated rats. In case of organs weight, the weight of the liver and weight of the pancreas were significantly (p < 0.001; p < 0.05, p < 0.01, p < 0.001) decreased in the rats treated with highest dose of EEAR (Alx+ EEAR 400) and combination therapy when compared to the disease control and fenofibrate treated rats. The current study demonstrates that combination therapy of EEAR and fenofibrate was more effective than that of monotherapy in controlling diabetes mellitus associated with cardiovascular diseases and hepatic dysfunction in alloxan-induced diabetic rats.
VL  - 5
IS  - 5-1
ER  -

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Author Information

Abdullah Al Mamun

Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Mahbubul Hossain

Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Ariful Islam

Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh
Sonia Zaman

Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Md. Sahab Uddin

Department of Pharmacy, Southeast University, Dhaka, Bangladesh

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Abdullah Al Mamun, Mahbubul Hossain, Ariful Islam, Sonia Zaman, Md. Sahab Uddin. (2016). Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats. Plant, 5(5-1), 1-12. https://doi.org/10.11648/j.plant.s.2017050501.11

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ACS Style

Abdullah Al Mamun; Mahbubul Hossain; Ariful Islam; Sonia Zaman; Md. Sahab Uddin. Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats. Plant. 2016, 5(5-1), 1-12. doi: 10.11648/j.plant.s.2017050501.11

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Abdullah Al Mamun, Mahbubul Hossain, Ariful Islam, Sonia Zaman, Md. Sahab Uddin. Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats. Plant. 2016;5(5-1):1-12. doi: 10.11648/j.plant.s.2017050501.11

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@article{10.11648/j.plant.s.2017050501.11,
  author = {Abdullah Al Mamun and Mahbubul Hossain and Ariful Islam and Sonia Zaman and Md. Sahab Uddin},
  title = {Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats},
  journal = {Plant},
  volume = {5},
  number = {5-1},
  pages = {1-12},
  doi = {10.11648/j.plant.s.2017050501.11},
  url = {https://doi.org/10.11648/j.plant.s.2017050501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.s.2017050501.11},
  abstract = {Diabetes mellitus is strongly connected with changes in lipid profile and also can cause damage of several organs like liver over a long period of time. The purpose of this study was designed to evaluate the hypolipidemic and hepatoprotective effects of ethanolic root extracts of Asparagus racemosus (EEAR) Linn. alone and in combination with a lipid lowering agent (fenofibrate) in alloxan-induced diabetic rats. Diabetes was induced in male Wister albino rats by the administration of single intra-peritoneal injection of alloxan monohydrate (120 mg/kg b.w.). Two different doses of EEAR (200 and 400 mg/kg b.w.) alone, fenofibrate (30 mg/kg b.w.) and a combination of EEAR (200 mg/kg b.w.) with fenofibrate (30 mg/kg b.w.) were administered orally for the period of 14 days. After the treatment period, hypolipidemic and hepatoprotective effects were determined by examining serum biochemical markers including total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), serum glutamate oxaloacetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT) and total protein (TP) with the aid of commercially available kits. The survival rate, body weight and organ weight were also measured. The ingestion of EEAR considerably (p th and 14th day as compared to that of disease control and fenofibrate treated rats. In case of organs weight, the weight of the liver and weight of the pancreas were significantly (p < 0.001; p < 0.05, p < 0.01, p < 0.001) decreased in the rats treated with highest dose of EEAR (Alx+ EEAR 400) and combination therapy when compared to the disease control and fenofibrate treated rats. The current study demonstrates that combination therapy of EEAR and fenofibrate was more effective than that of monotherapy in controlling diabetes mellitus associated with cardiovascular diseases and hepatic dysfunction in alloxan-induced diabetic rats.},
 year = {2016}
}

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TY  - JOUR
T1  - Asparagus racemosus Linn. Potentiates the Hypolipidemic and Hepatoprotective Activity of Fenofibrate in Alloxan-Induced Diabetic Rats
AU  - Abdullah Al Mamun
AU  - Mahbubul Hossain
AU  - Ariful Islam
AU  - Sonia Zaman
AU  - Md. Sahab Uddin
Y1  - 2016/10/18
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.s.2017050501.11
DO  - 10.11648/j.plant.s.2017050501.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 1
EP  - 12
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.s.2017050501.11
AB  - Diabetes mellitus is strongly connected with changes in lipid profile and also can cause damage of several organs like liver over a long period of time. The purpose of this study was designed to evaluate the hypolipidemic and hepatoprotective effects of ethanolic root extracts of Asparagus racemosus (EEAR) Linn. alone and in combination with a lipid lowering agent (fenofibrate) in alloxan-induced diabetic rats. Diabetes was induced in male Wister albino rats by the administration of single intra-peritoneal injection of alloxan monohydrate (120 mg/kg b.w.). Two different doses of EEAR (200 and 400 mg/kg b.w.) alone, fenofibrate (30 mg/kg b.w.) and a combination of EEAR (200 mg/kg b.w.) with fenofibrate (30 mg/kg b.w.) were administered orally for the period of 14 days. After the treatment period, hypolipidemic and hepatoprotective effects were determined by examining serum biochemical markers including total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), serum glutamate oxaloacetate transaminases (SGOT), serum glutamate pyruvate transaminases (SGPT) and total protein (TP) with the aid of commercially available kits. The survival rate, body weight and organ weight were also measured. The ingestion of EEAR considerably (p th and 14th day as compared to that of disease control and fenofibrate treated rats. In case of organs weight, the weight of the liver and weight of the pancreas were significantly (p < 0.001; p < 0.05, p < 0.01, p < 0.001) decreased in the rats treated with highest dose of EEAR (Alx+ EEAR 400) and combination therapy when compared to the disease control and fenofibrate treated rats. The current study demonstrates that combination therapy of EEAR and fenofibrate was more effective than that of monotherapy in controlling diabetes mellitus associated with cardiovascular diseases and hepatic dysfunction in alloxan-induced diabetic rats.
VL  - 5
IS  - 5-1
ER  -

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