Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of <i>Botryodiplodia theobromae</i> (Pat.)

John Meomikem Ehiobu

doi:doi:10.11648/j.jdmp.20170305.11

| Peer-Reviewed

Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.)

John Meomikem Ehiobu

Published in Journal of Diseases and Medicinal Plants (Volume 3, Issue 5)

Received: 10 October 2017 Accepted: 28 October 2017 Published: 20 November 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The control of fungal pathogens of economic crops in soil is crucial to sustainable food production and distribution. In this study, the effect of aqueous fractions of petroleum hydrocarbons was investigated on the growth and sporulation of Botryodiplodia theobromae (Pat.), a common fungal pathogen isolated from rotten cassava tubers. The fungal isolates were subjected to graded concentrations of the water-soluble fraction (WSF) of crude oil and monitored for their effects on the mycelial growth and spore germination using standard mycological media and techniques. Results revealed that the biological effects of the chemical fraction were concentration dependent and significant at P = 0.01 and 0.05 when compared with the controls. After 5 h, the least inhibitory effect against the spore germination was observed by the 1 mL WSF concentration with 87% spore germination. The effects were significantly different (P<0.01) at WSF concentrations of 7.0-9.0 mg/mL with percentage germination values of 44%, 46% and 29% respectively. The mycelial growths were also significantly reduced with the highest inhibition being 73% at 9.0 mg/mL. The mycelial dry weight remained significantly unchanged (P>0.05) at higher concentrations. Findings from this study suggest that aqueous soluble fractions of petroleum hydrocarbons can significantly inhibit the spores and mycelium growth of B. theobromae (Pat.). Thus, they could be exploited as template for biosynthesis of potential fungicide against the test phytopathogens.

Published in	Journal of Diseases and Medicinal Plants (Volume 3, Issue 5)
DOI	10.11648/j.jdmp.20170305.11
Page(s)	83-87
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Fungicide, Botryodiplodia theobromae (Pat.), Hydrocarbon, Water

References

[1]	Domsch, K. H., W. and Anderson, T. H. (2007). Compendium of Soil Fungi. 2nd Edition. Cornell University. England.
[2]	Ohene-Mensah G. (2012). Characterization of Botryodiplodia theobromae isolates affecting cocoa, mango, banana and yam in Ghana. A thesis resented to the Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, in Partial fulfillment of requirement of M.Sc. (Hons) Degree in Biotechnology, Ghana. pp. 1-67.
[3]	Jones, D. R. and Stove, R. H. (2000). Fungal diseases of banana fruit, pre-harvest disease. In: Diseases of Banana, Abacá and Enset, D. R. Jones (Ed.). CABI Publishing. Wallingford, UK. pp.173-190.
[4]	Phipps, P. M. and Porter, D. M. (1998). Collar rot of peanut caused by Lasiodiplodia theobromae. Plant Dis. 1998; 82:1205-1209.
[5]	Twumasi, P., Ohene-Mensah, G. and Moses, E. (2014). The rot fungus Botryodiplodia theobromae strains cross infect cocoa, mango, banana and yam with significant tissue damage and economic losses. Afr. J. Agric. Res. 9(6): 613-619.
[6]	Syed, R. N., Mansha, N., Khaskheli, M. A., Khanzada, M. A. and Lodhi, A. M. (2014). Chemical control stem end rot of mango caused by of Lasiodiplodia theobromae Pak. J. Phytopathol. 26 (02): 201-206.
[7]	Rossman, A. Y., Palm, M. E. and Spielman, L. J. (1997). A literature guide for theidentification ofplant pathogenic fungi. Minnesota, USA, APS Press. 24pp.
[8]	Markson, A. A., Amadioha, A. C., Omosun, G., Madunagu, B. E., Udo, S. E and Umana, E. J. (2012). Control of Botryodiplodia theobromae causing Tissue Rot of White Yam (Dioscorea rotundata Poir). Schol. J. Agric. Sci. 2(1):1-7.
[9]	SAS. (2002). Statistical Analytical System. User’s guide statistics version 91, SAS Institute Inc. Raleigh, N. Y. USA.
[10]	Edema, N. E., Okoloko, G. E. and Ogbogidi, M. O. (2007). Physico-chemical characteristics of the water-soluble fraction of Ogini well-head crude oil and the effects on Pistia stratiotes Linn (Water lettuces). American-Eurasian J. Agric. Environ Sci., 2007; 2(6):633-638.
[11]	Sadani, M., Movahedian, H.,Faraji, M., Jaberian, B., Hajian, M. and Mehrizi, E. A. (2011). Effects of Physical and Chemical characteristics of water on Toxicity of crude oil water soluble fraction on Daphnia Magna. World Appl. Sci. J. 14 (11):1744-1747.
[12]	Bamidele, J. F. and Eshagberi, G. O. (2015). Effects of Water Soluble Fractions of Crude Oil,Diesel Fuel and Gasoline on Salvinia nymphellula (Desv). J. Nat. Sci. Res. 5(14):31-37.
[13]	Overton, E. B., Sharp, W. D. and Roberts, P. (1994). Toxicity of Petroleum. In: BasicEnvironmental Toxicology. Cockerham LG and Shama BS (Eds.). First Edition CRC Press Inc. New York. pp 133-156.
[14]	Nweke, F. U. (2015). Effect of some plant leaf extracts on mycelia growth and spore germination of Botryodiplodia theobromae causal organism of yam tuber rot. J. Bio. Agric. and Healthcare. 5(8): 67-71.
[15]	Anuagasi, C. L., Onuorah, J. A. and Okigbo, R. N. (2017). Fungal pathogens affecting seedlingsof Gmelina arborea Roxb and Tectona grandis L. F. and effect of three plant extracts. Int. J. Agric. Technol. 13(3): 307-330.
[16]	Ekhuemelo, C. and Yaaju, M. D. (2017). Identification and management of fungi associated with crown rot of banana in Makurdi, Benue State, Nigeria. Nig. J. Agric. Food Environ. 13(2):50-55.
[17]	Shiriki, D., Ubwa, S. T. and Shambe, T. (2015) Isolation of nine microorganisms from rotten Dioscorea rotundata (White yam) and antimicrobial sensitivity test with five plant pxtracts. Food Nutri. Sci.6: 825-835.
[18]	Akinde, S. B., Adeniyi, M. A., Adebunmi, A. A., Oluwajide, O. O. and Ogunnaike, O. O. (2017). Comparative effectiveness of chemical biocides and Acalypha wilkesiana leaf extract against postharvest fungal deteriogens of sweet orange (Citrus sinensis) fruits. Egyptian J. Basic Appl. Sci. 4: 143–152.
[19]	Javaid, A., Shoaib, A. and Akhtar, N. (2008). In vitro chemical control of Botryodiplodia theobromae, the cause of Dying Back disease of mango. Pak. J. Phytopathol. 20(2): 195-199.
[20]	Shuping, D. S. S. and Eloff, J. N. (2017). The use of plants to protect plants and food againstfungal pathogens: a Review. Afr. J. Trad. Complement. Alt. Med. 14 (4):120-127.
[21]	Anjum M. A, Ahmed, N, Babita, C. H. and Gupta, P. (2016) Plant extracts in post-harvest disease management of fruits and vegetables-A Review. J. Food Process Technol. 7: 592. doi:10.4172/2157-7110.1000592.

Cite This Article

Plain Text BibTeX RIS

APA Style

John Meomikem Ehiobu. (2017). Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.). Journal of Diseases and Medicinal Plants, 3(5), 83-87. https://doi.org/10.11648/j.jdmp.20170305.11

Copy | Download

ACS Style

John Meomikem Ehiobu. Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.). J. Dis. Med. Plants 2017, 3(5), 83-87. doi: 10.11648/j.jdmp.20170305.11

Copy | Download

AMA Style

John Meomikem Ehiobu. Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.). J Dis Med Plants. 2017;3(5):83-87. doi: 10.11648/j.jdmp.20170305.11

Copy | Download

@article{10.11648/j.jdmp.20170305.11,
  author = {John Meomikem Ehiobu},
  title = {Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.)},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {3},
  number = {5},
  pages = {83-87},
  doi = {10.11648/j.jdmp.20170305.11},
  url = {https://doi.org/10.11648/j.jdmp.20170305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170305.11},
  abstract = {The control of fungal pathogens of economic crops in soil is crucial to sustainable food production and distribution. In this study, the effect of aqueous fractions of petroleum hydrocarbons was investigated on the growth and sporulation of Botryodiplodia theobromae (Pat.), a common fungal pathogen isolated from rotten cassava tubers. The fungal isolates were subjected to graded concentrations of the water-soluble fraction (WSF) of crude oil and monitored for their effects on the mycelial growth and spore germination using standard mycological media and techniques. Results revealed that the biological effects of the chemical fraction were concentration dependent and significant at P = 0.01 and 0.05 when compared with the controls. After 5 h, the least inhibitory effect against the spore germination was observed by the 1 mL WSF concentration with 87% spore germination. The effects were significantly different (P0.05) at higher concentrations. Findings from this study suggest that aqueous soluble fractions of petroleum hydrocarbons can significantly inhibit the spores and mycelium growth of B. theobromae (Pat.). Thus, they could be exploited as template for biosynthesis of potential fungicide against the test phytopathogens.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.)
AU  - John Meomikem Ehiobu
Y1  - 2017/11/20
PY  - 2017
N1  - https://doi.org/10.11648/j.jdmp.20170305.11
DO  - 10.11648/j.jdmp.20170305.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 83
EP  - 87
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20170305.11
AB  - The control of fungal pathogens of economic crops in soil is crucial to sustainable food production and distribution. In this study, the effect of aqueous fractions of petroleum hydrocarbons was investigated on the growth and sporulation of Botryodiplodia theobromae (Pat.), a common fungal pathogen isolated from rotten cassava tubers. The fungal isolates were subjected to graded concentrations of the water-soluble fraction (WSF) of crude oil and monitored for their effects on the mycelial growth and spore germination using standard mycological media and techniques. Results revealed that the biological effects of the chemical fraction were concentration dependent and significant at P = 0.01 and 0.05 when compared with the controls. After 5 h, the least inhibitory effect against the spore germination was observed by the 1 mL WSF concentration with 87% spore germination. The effects were significantly different (P0.05) at higher concentrations. Findings from this study suggest that aqueous soluble fractions of petroleum hydrocarbons can significantly inhibit the spores and mycelium growth of B. theobromae (Pat.). Thus, they could be exploited as template for biosynthesis of potential fungicide against the test phytopathogens.
VL  - 3
IS  - 5
ER  -

Copy | Download

Author Information

John Meomikem Ehiobu

Department of Biology, College of Education, Agbor, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

John Meomikem Ehiobu. (2017). Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.). Journal of Diseases and Medicinal Plants, 3(5), 83-87. https://doi.org/10.11648/j.jdmp.20170305.11

Copy | Download

ACS Style

John Meomikem Ehiobu. Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.). J. Dis. Med. Plants 2017, 3(5), 83-87. doi: 10.11648/j.jdmp.20170305.11

Copy | Download

AMA Style

John Meomikem Ehiobu. Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.). J Dis Med Plants. 2017;3(5):83-87. doi: 10.11648/j.jdmp.20170305.11

Copy | Download

@article{10.11648/j.jdmp.20170305.11,
  author = {John Meomikem Ehiobu},
  title = {Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.)},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {3},
  number = {5},
  pages = {83-87},
  doi = {10.11648/j.jdmp.20170305.11},
  url = {https://doi.org/10.11648/j.jdmp.20170305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170305.11},
  abstract = {The control of fungal pathogens of economic crops in soil is crucial to sustainable food production and distribution. In this study, the effect of aqueous fractions of petroleum hydrocarbons was investigated on the growth and sporulation of Botryodiplodia theobromae (Pat.), a common fungal pathogen isolated from rotten cassava tubers. The fungal isolates were subjected to graded concentrations of the water-soluble fraction (WSF) of crude oil and monitored for their effects on the mycelial growth and spore germination using standard mycological media and techniques. Results revealed that the biological effects of the chemical fraction were concentration dependent and significant at P = 0.01 and 0.05 when compared with the controls. After 5 h, the least inhibitory effect against the spore germination was observed by the 1 mL WSF concentration with 87% spore germination. The effects were significantly different (P0.05) at higher concentrations. Findings from this study suggest that aqueous soluble fractions of petroleum hydrocarbons can significantly inhibit the spores and mycelium growth of B. theobromae (Pat.). Thus, they could be exploited as template for biosynthesis of potential fungicide against the test phytopathogens.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Effect of Water-Soluble Fraction of Petroleum Hydrocarbon on the Growth and Sporulation of Botryodiplodia theobromae (Pat.)
AU  - John Meomikem Ehiobu
Y1  - 2017/11/20
PY  - 2017
N1  - https://doi.org/10.11648/j.jdmp.20170305.11
DO  - 10.11648/j.jdmp.20170305.11
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 83
EP  - 87
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20170305.11
AB  - The control of fungal pathogens of economic crops in soil is crucial to sustainable food production and distribution. In this study, the effect of aqueous fractions of petroleum hydrocarbons was investigated on the growth and sporulation of Botryodiplodia theobromae (Pat.), a common fungal pathogen isolated from rotten cassava tubers. The fungal isolates were subjected to graded concentrations of the water-soluble fraction (WSF) of crude oil and monitored for their effects on the mycelial growth and spore germination using standard mycological media and techniques. Results revealed that the biological effects of the chemical fraction were concentration dependent and significant at P = 0.01 and 0.05 when compared with the controls. After 5 h, the least inhibitory effect against the spore germination was observed by the 1 mL WSF concentration with 87% spore germination. The effects were significantly different (P0.05) at higher concentrations. Findings from this study suggest that aqueous soluble fractions of petroleum hydrocarbons can significantly inhibit the spores and mycelium growth of B. theobromae (Pat.). Thus, they could be exploited as template for biosynthesis of potential fungicide against the test phytopathogens.
VL  - 3
IS  - 5
ER  -

Copy | Download